Rchr
J-GLOBAL ID:200901000887675536
Update date: Aug. 04, 2022
Makino Shin-ichi
マキノ シンイチ | Makino Shin-ichi
Affiliation and department:
Job title:
Professor
Homepage URL (1):
https://www.kct.ac.jp/
Research field (4):
Applied biochemistry
, Molecular biology
, Functional biochemistry
, Molecular biochemistry
Research keywords (6):
Wheat cell-free system
, RNA ligase
, Genomic analysis
, Protein synthesis
, RNA
, RNA
Research theme for competitive and other funds (3):
- 2018 - 2022 Establishment of a synthesis method for single-stranded circular viroid RNAs and characterization of their molecular and chemical properties
- コムギ胚芽無細胞タンパク質合成系におけるRNA
- RNA in a wheat cell-free translation system
Papers (34):
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Benjamin B. Minkoff, Shin-ichi Makino, Miyoshi Haruta, Emily T. Beebe, Russell L. Wrobel, Brian G. Fox, Michael R. Sussman. A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA. JOURNAL OF BIOLOGICAL CHEMISTRY. 2017. 292. 14. 5932-5942
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Bo Li, Shin-ichi Makino, Emily T. Beebe, Daisuke Urano, David J. Aceti, Tina M. Misenheimer, Jonathan Peters, Brian G. Fox, Alan M. Jones. Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein. PROTEIN EXPRESSION AND PURIFICATION. 2016. 126. 33-41
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Henry Sackin, Mikheil Nanazashvili, Shin-ichi Makino. Direct injection of cell-free Kir1.1 protein into Xenopus oocytes replicates single-channel currents derived from Kir1.1 mRNA. CHANNELS. 2015. 9. 4. 196-199
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Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, et al. Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies. Journal of structural and functional genomics. 2015. 16. 2. 67-80
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Emily T. Beebe, Shin Ichi Makino, John L. Markley, Brian G. Fox. Automated cell-free protein production methods for structural studies. Methods in molecular biology (Clifton, N.J.). 2014. 1140. 117-135
more...
MISC (14):
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Shin-ichi MAKINO. Trials for utilizing the merits of classroom situation on teaching Basic Biology. 2019. 52. 59-64
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Katarzyna Gromek, David Aceti, Emily Beebe, Craig Bingman, Uyen Chu, Brian Fox, Ronnie Frederick, Lianwang Guo, Shinichi Makino, Hannah Meddaugh, et al. Expression and purification optimization of active sigma 1 receptor membrane protein. PROTEIN SCIENCE. 2012. 21. 213-213
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George N. Phillips, John Primm, David Aceti, Craig A. Bingman, Ronnie Frederick, Shin-ichi Makino, Francis Peterson, Frank Vojtik, Russel Wrobel, Zsolt Zolnai, et al. Technology Development Highlights Generated from the Center for Eukaryotic Structural Genomics. BIOPHYSICAL JOURNAL. 2010. 98. 3. 250A-251A
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MAKINO SHIN'ICHI, SAWASAKI TATSUYA, ENDO YAETA, TAKAI KAZUYUKI. コムギRNAリガーゼの各活性ドメインへの分割と特性の解析. 生化学. 2007. 2P-1137
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KUGA TAKAHIRO, MAKINO SHIN'ICHI, ENDO YAETA, TAKAI KAZUYUKI. コムギはい芽リボソームディスプレイ法によるGST変異体ランダムライブラリーのスクリーニングの試み. 日本分子生物学会年会講演要旨集. 2005. 28th. 765
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Books (1):
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応用微生物学 第3版
文永堂出版 2016 ISBN:9784830041310
Lectures and oral presentations (34):
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In vitro翻訳によるナノディスク産生のための膜スキャフォールドタンパク質の最適化
(日本分子生物学会年会プログラム・要旨集(Web) 2014)
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Advantages of Wheat-Germ Cell-Free Translation for Membrane Protein Research
(PSI:Biology Technologies Workshop 2012)
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Strategies for Membrane Protein Preparation at the Transmembrane Protein Center Utilizing Cell-free and Cell-based Systems
(Keystone Symposium on High-Throughput Structural Biology 2012)
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Recent Advances in Cell-Free and Cell-Based Protein Production at the Transmembrane Protein Center
(The 6th International Conference on Structural Genomics 2011)
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Recent Advances in the Utilization of the Wheat Germ Cell-Free Expression System
(The 24th Annual Symposium of the Protein Society 2010)
more...
Education (3):
- 1996 - 1999 The University of Tokyo
- 1994 - 1996 The University of Tokyo
- - 1994 The University of Tokyo
Professional career (1):
- Ph. D. (The University of Tokyo)
Work history (8):
- 2021/04 - 現在 Kitakyushu National College of Technology Department of Creative Engineering Professor
- 2015/04 - 2021/03 National Institute of Technology, Kitakyushu College Department of Creative Engineering Associate Professor
- 2012/11 - 2015/03 University of Wisconsin-Madison Biochemistry Assistant Scientist
- 2008/02 - 2012/10 University of Wisconsin-Madison Center for Eukaryotic Structural Genomics Research Associate
- 2005/04 - 2008/01 Ehime University Cell-Free Science and Technology Research Center
- 2002/06 - 2005/03 Ehime University
- 2001/04 - 2002/05 三菱化学生命科学研究所 特別研究員
- 1999/04 - 2001/03 通商産業省 工業技術院生命工学工業技術研究所 特別技術補助職員
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Association Membership(s) (3):
日本生化学会
, 日本農芸化学会
, 日本分子生物学会
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