Kamachi Yusuke

カマチユウスケ | Kamachi Yusuke

Affiliation and department：

Research field (2)： Developmental biology , Molecular biology

Research keywords (5)： Genome editing , Developmental biology , Zebrafish , Transcription factor , Gene regulation

Research theme for competitive and other funds (31)：

2021 - 2024 発光タグHiBiTの高機能化・多機能化を通した新規発生遺伝子解析手法の確立
2018 - 2021 In vivo function analysis of Sox-partner factor complexes in cell fate switch
2015 - 2018 Development and application of methods for identifying protein-DNA interactions in a tissue-specific manner
2013 - 2017 Regulation of microRNA genes by Sox transcription factors
2011 - 2015 Genomic target site recognition by SOX-partner factor complexes that underlies switching mechanisms in cell differentiation

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Papers (48)：

Yusuke Kamachi, Atsuo Kawahara. CRISPR-Cas9-Mediated Genome Modifications in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2023. 2637. 313-324
Keita Okada, Kanae Aoki, Teruyuki Tabei, Kota Sugio, Katsunori Imai, Yuki Bonkohara, Yusuke Kamachi. Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9. Nucleic Acids Research. 2022. 50. 5. 2854-2871
Deshani C. Ranawakage, Keita Okada, Kota Sugio, Yuya Kawaguchi, Yuki Kuninobu-Bonkohara, Takuya Takada, Yusuke Kamachi. Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor. Frontiers in Cell and Developmental Biology. 2021. 8
Deshani C. Ranawakage, Takuya Takada, Yusuke Kamachi. HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions. Scientific Reports. 2019. 9
Yu Okamoto, Naoko Nishimura, Kazunari Matsuda, Deshani C. Ranawakage, Yusuke Kamachi, Hisato Kondoh, Masanori Uchikawa. Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development. Development Growth and Differentiation. 2018. 60. 3. 133-145

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MISC (20)：

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Education (3)：

Professional career (1)：

Work history (5)：

2015/06 - 現在 Kochi University of Technology School of Environmental Science and Engineering Professor
2007/04 - 2015/05 Osaka University Graduate School of Frontier Biosciences Associate Professor
2002/04 - 2007/03 Osaka University Graduate School of Frontier Biosciences Associate Professor
2001/05 - 2002/06 University of Minnesota Department of Genetics, Cell Biology and Development Visiting Researcher
1994/04 - 2002/03 Osaka University Institute for Molecular and Cellular Assistant professor

Association Membership(s) (2)：

日本発生生物学会 , 日本分子生物学会

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