Ookuri Takatoshi

オオクリタカトシ | Ookuri Takatoshi

Affiliation and department：
Job title： Professor
Homepage URL (1)： http://www.ph.sojo-u.ac.jp/~ohkuri/index.html

Research field (4)： Pharmaceuticals - analytical and physicochemistry , Immunology , Pharmaceuticals - analytical and physicochemistry , Pharmaceuticals - health and biochemistry

Research keywords (1)：抗体 Fab

Research theme for competitive and other funds (8)：

2020 - 2023 超耐熱化蛋白質融合抗体フラグメントの創製
2016 - 2020 Thermostabilization of Fab and its application to medicine
2013 - 2017 Study on structure, function and gene regulation of Protobothrops flavoviridis snake venom phospholipase A2 isozymes
2011 - 2012 Analysis ofthe relationship between tchoenformational stabilityof phospholipaseA2 and the amount of IgG production.
2010 - 2012 Molecular mechanisms of transcriptional regulationProitnobothrops flavoviridis venom gland.

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Papers (49)：

Moeka Yoshikawa, Miki Senda, Hitomi Nakamura, Naoko Oda-Ueda, Tadashi Ueda, Toshiya Senda, Takatoshi Ohkuri. Stabilization of adalimumab Fab through the introduction of disulfide bonds between the variable and constant domains. Biochemical and Biophysical Research Communications. 2024. 700. 149592-149592
Kosuke Oyama, Makoto Nakakido, Takatoshi Ohkuri, Hitomi Nakamura, Kouhei Tsumoto, Tadashi Ueda. Enhancing thermal stability in the CH2 domain to suppress aggregation through the introduction of simultaneous disulfide bonds in Pichia pastoris. Protein science : a publication of the Protein Society. 2023. 32. 12. e4831
Moeka Yoshikawa, Hitomi Nakamura, Naoko Oda-Ueda, Tadashi Ueda, Takatoshi Ohkuri. Effect of an intermolecular disulfide bond introduced into the first loop of CH1 domain of Adalimumab Fab on thermal stability and antigen-binding activity. The Journal of Biochemistry. 2022. 172. 1. 49-56
Hitomi Nakamura, Moeka Yoshikawa, Naoko Oda-Ueda, Tadashi Ueda, Takatoshi Ohkuri. A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab. Scientific Reports. 2021. 11. 1
Oyama K, Ohkuri T, Ochi J, Caaveiro JMM, Ueda T. Abolition of aggregation of CH 2 domain of human IgG1 when combining glycosylation and protein stabilization. Biochem Biophys Res Commun. 2021. 18. 558. 114-119

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MISC (16)：

Yoshikawa M, Nakamura H, Oda-Ueda N, Ohkuri T. Analysis of thermostability for seven Phe to Ala and six Pro to Gly mutants in the Fab constant region of adalimumab. The Journal of Biochemistry. 2023. 174. 4. 345-353
中村仁美, 大栗誉敏, 服部正策, 上田直子. Neutralizing effects of monoclonal antibodies against the myotoxic factor (BPII) from Protobothrops flavoviridis venom and the development of related Fab fragments. 日本薬学会年会要旨集(CD-ROM). 2020. 140th
中村仁美, 上田直子, 植田正, 大栗誉敏. 分子間SS結合を有したFabのC末端特異的PEG修飾体の調製. 日本細胞生物学会大会(Web). 2019. 71st
中村仁美, 木吉真人, 安楽誠, 上田直子, 植田正, 大栗誉敏. ヒト型Fab定常領域への糖鎖配列の導入と糖鎖が及ぼす影響. 日本生化学会大会(Web). 2019. 92nd
植田正, 大栗誉敏, 阿部義人, 村上茂. 試験管内における蛋白質の凝集抑制に及ぼすタウリンの効果. タウリンリサーチ. 2018. 4. 1. 6-8

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Books (2)：

Protein Folding: New Research.
Nova Science Publishers,Inc. 2006
薬系免疫学(第12章)
南江堂 2005

Lectures and oral presentations (47)：

抗体医薬品アダリムマブFabにおけるVH-CH1及びVL-CLドメイン間へのSS結合の導入による安定性の上昇
(第40回日本薬学会九州山口支部大会 2023)
ヒト型抗体Fab安定化のための新規分子間SS結合導入部位の探索
(第40回日本薬学会九州山口支部大会 2023)
抗体医薬アダリムマブFabの可変-定常ドメイン間へのSS結合導入による熱安定性の上昇
(第23回日本蛋白質科学会年会 2023)
セルトリズマブFab可変領域へのアミノ酸変異導入による安定化
(日本薬学会第143年会 2023)
抗体医薬アダリムマブFabの定常領域における7種のPhe→Ala変異体及びPhe-Pro配列上の6種のPro→Gly変異体の熱安定性解析
(第22回日本蛋白質科学会年会 2022)

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Education (3)：

1996 - 1999 Kyushu University Graduate School, Division of Pharmaceutical Sciences
1994 - 1996 Kyushu University Graduate School, Division of Pharmaceutical Sciences
1990 - 1994 Fukuoka University Faculty of Pharmaceutical Science

Professional career (1)：

博士(薬学) (九州大学大学院)

Work history (5)：

2020/04 - 現在 FACULTY OF PHARMACEUTICAL SCIENCES FACULTY OF PHARMACEUTICAL SCIENCES Department of Pharmaceutical Sciences
2009/10 - 2020/03 FACULTY OF PHARMACEUTICAL SCIENCES FACULTY OF PHARMACEUTICAL SCIENCES Department of Pharmaceutical Sciences
2008/04 - 2009/09 Assistant Professor
2002/04 - 2008/03 Research Assistant
1999/04 - 2002/03 Researcher

Awards (2)：

2014/10 - 日本生化学会日本生化学会JB論文賞 Characterization of deamidation at Asn138 in L-chain of recombinant humanized Fab expressed from Pichia pastoris.
2011/05 - 平成23年度日本生化学会九州支部学術奨励賞リゾチームの蛋白質工学的基盤研究とその応用

Association Membership(s) (3)：

日本薬学会 , 日本蛋白質科学会 , 日本生化学会

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