Rchr
J-GLOBAL ID：200901036042797475
Update date: Apr. 03, 2024
Sawasaki Tatsuya
サワサキ タツヤ | Sawasaki Tatsuya
Affiliation and department：
Job title：
Professor
Homepage URL (1)：
http://www.pros.ehime-u.ac.jp/cell-free/
Research field (7)：
Systems genomics
, Functional biochemistry
, Tumor biology
, Other
, Structural biochemistry
, Plants: molecular biology and physiology
, Applied biofunctional and bioprocess engineering
Research keywords (37)：
PROTAC
, Protein degrader
, thalidomide
, Molecular glue
, proximity biotinylation enzyme
, 無細胞タンパク質合成
, 蛋白質生産
, コムギ胚芽
, プロテインアレイ
, 無細胞蛋白質合成系
, 蛋白質代謝
, タンパク質
, ユビキチン
, プロテオリシス
, E3リガーゼ
, 細胞内シグナル
, 細胞死
, プロテインカイネース
, カスパーゼ
, プロテアーゼ
, 切断
, Alpha Screen
, カスパーゼ3
, ユビキチン化
, RING
, がん抑制タンパク質
, 炎症
, 無脂肪タンパク質合成
, 蛋白質ライブラリー
, ポリユビキチン鎖
, シグナル伝達
, 翻訳
, cell-free
, 蛋白質ネットワーク
, 無細胞蛋白質合成
, Protein network
, Cell-free protein production
Research theme for competitive and other funds (41)：
- 2022 - 2028 先端モデル動物支援プラットフォーム
- 2022 - 2026 複合型ユビキチン鎖を標的とした筋萎縮性側索硬化症の病態解明と創薬シーズの開拓
- 2020 - 2024 Evolutionary dynamics of cis-trans factors on the regulation of plant gene cluster for diterpenoid momilactone.
- 2020 - 2024 Research on odorant reception system in plants
- 2021 - 2023 Development of antibody-fusion proximity biotinylation enzyme for analysis of virus entry
- 2020 - 2023 Comprehensive identification of responsible regulatory molecules that define the seizure periodicity and inflammatory diversity of autoinflammatory diseases
- 2019 - 2022 Development of a technique for identifying drug-dependent interacting proteins using cell-free based human protein arrays.
- 2017 - 2022 コムギ無細胞系による構造解析に適した複合体タンパク質生産・調製技術と低分子抗体作製技術の創出
- 2016 - 2021 Development of cell-free proteome technology for analysis of the signal transduction based on protein molecule interaction
- 2017 - 2020 Comparative survey of endogenous and environmental factors that activate autoinflammatory disease responsible gene products in Turkey and Japan
- 2016 - 2019 Preparation of alpaca-derived nanobody which activatites ghrelin receptor and its application to the crystal structure analysis
- 2016 - 2019 Analysis of a novel PACMA compound as a candidate drug for ovarian cancer
- 2015 - 2019 A study of structural impairments of STAS domain caused by common p.H723R mutation of SLC26A4 in patients with Pendred syndrome
- 2016 - 2019 Development of houndshark nanodoy by combination of wheat cell-free and NGS technologies
- 2019 - 2019 NLRP3インフラマソーム機能を調節する新規分子の機能解析
- 2016 - 2018 Identification of proteolytic signaling pathways regulating reprogramming during iPS cell generation
- 2016 - 2018 Functional analysis of the role of plant tyrosine protien kinase for gibberellin signalling.
- 2015 - 2018 Development of new dual inhibitors for c-Met targeting both allosteric and catalytic sites
- 2015 - 2018 Screening for interactors of linear ubiquitin chain assembly complex (LUBAC), and studies on their physiological functions
- 2015 - 2018 Regulation of cellular signal transduction by novel immunological factor
- 2015 - 2017 Development of the prevention method for steroid-induced osteonecrosis using screening system of cell-free technology for protein analyses
- 2014 - 2017 A prospective study in order to identify inflammatory attack triggers in autoinflammatory diseases
- 2014 - 2017 A comparative study of intrinsic factor activating inflammasome between Turkey and Japan
- 2012 - 2017 コムギ無細胞合成系による蛋白質生産支援・高親和抗体構築技術開発
- 2014 - 2016 Basic technology development for Shark nanobody using antigen protein produced by wheat cell-free system
- 2013 - 2016 Discovery of a novel caspase-1 substrate using a cell-free based protein array
- 2013 - 2015 無細胞蛋白質アレイによるポリユビキチン鎖依存シグナル伝達経路の網羅的同定と解析
- 2012 - 2014 Screening of novel anti-citrullinated protein antibodies for rheumatoid arrhtiris by protein array
- 2011 - 2013 無細胞蛋白質アレイを基盤とした細胞がん化E3リガーゼの網羅的同定と解析
- 2011 - 2013 Comprhensive examination for serum autoantigens in endogenous uveitis patients
- 2011 - 2012 Analysis of risk factors associated with non-response to infliximab treatment for Behcet's disease
- 2010 - 2012 Comprehensive identification of caspase-dependent signal transduction pathway using cell-free protein array
- 2010 - 2011 Molecular mechanism of auto-translocation of membrane anchored EGFR ligand into the nuclear membrane and its diagnostic application of cancer
- 2009 - 2011 Identification of RBC receptors against malaria parasite molecules in the merozoite apical organelle
- 2009 - 2010 Regulation of Arabidopsis defense responses against herbivory and abiotic stresses by calcium-dependent protein phosphorylation and ubiquitnation
- 2007 - 2008 カスパーゼをモデルとした蛋白質代謝ネットワーク解明に向けた基礎的技術開発
- 2002 - 2007 無細胞系を用いた網羅的タンパク質機能解析
- 2002 - 2007 Genome-wide protein functional analysis based on cell-free system
- 2001 - 2002 Production of selenomethionine labeled proteins by cell-free translation system and application to X-ray crystal structure analysis.
- 植物細胞死機構に関する研究
- Studies in mechanism of Plant cell Death
Show all
Papers (208)：
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Yuki Utsugi, Ken Nishimura, Satoshi Yamanaka, Kohei Nishino, Hidetaka Kosako, Tatsuya Sawasaki, Hideyuki Shigemori, Thomas J Wandless, Yusaku Miyamae. Ubiquitin-Derived Fragment as a Peptide Linker for the Efficient Cleavage of a Target Protein from a Degron. ACS chemical biology. 2024
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Kohdai Yamada, Ryouhei Shioya, Kohei Nishino, Hirotake Furihata, Atsushi Hijikata, Mika K. Kaneko, Yukinari Kato, Tsuyoshi Shirai, Hidetaka Kosako, Tatsuya Sawasaki. Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding. Nature Communications. 2023. 14. 1
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Xiaofang Li, Kenji Nakashima, Masahiko Ito, Mami Matsuda, Takeshi Chida, Kazumasa Sekihara, Hirotaka Takahashi, Takanobu Kato, Tatsuya Sawasaki, Tetsuro Suzuki. SRPKIN-1 as an inhibitor against hepatitis B virus blocking the viral particle formation and the early step of the viral infection. Antiviral research. 2023. 220. 105756-105756
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坂口 詩穏, 高橋 宏隆, 及川 大輔, 徳永 文稔, 西野 耕平, 小迫 英尊, 澤崎 達也. ウイルスRNA受容体MDA5を介したIFN産生シグナルを制御する脱ユビキチン化酵素の機能解析. 日本生化学会大会プログラム・講演要旨集. 2023. 96回. [1T17a-609)]
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Hiroyuki Takeda, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, et al. VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica). Frontiers in Bioengineering and Biotechnology. 2023. 11. 1265582
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MISC (392)：
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澤崎 達也. いま知りたい!!近接依存性標識法が拓く相互作用解析の新時代 概論 近接依存性標識法の特徴と解析例. 実験医学. 2023. 41. 11. 1769-1775
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堀凌輔, 篠原颯太, 野澤彰, 西野耕平, 小迫英尊, 澤崎達也. Development of technology for identification of proteins interacting with a target protein in plants using a proximity biotinylation enzyme, AirID. 日本植物生理学会年会(Web). 2023. 64th
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海老根一生, 海老根一生, 藤本優, 室啓太, 武内秀憲, 野澤彰, 澤崎達也, 東山哲也, 上田貴志, 上田貴志. Analysis of PICALM2 and pollen-specific VAMP72 members in Arabidopsis. 日本植物生理学会年会(Web). 2023. 64th
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西田快世, 仲村友介, 宮地朋子, 山上あゆみ, 野澤彰, 澤崎達也, 鈴木健裕, 堂前直, 宮川拓也, 松井南, et al. Analysis for phosphorylation dynamics of BIL7 that promotes plant growth in brassinosteroid signaling. 日本植物生理学会年会(Web). 2023. 64th
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山本悠介, 佐藤有美香, 石本晶也, 野澤彰, 小迫英尊, 澤崎達也, 関藤孝之, 関藤孝之, 河田(河野)美幸, 河田(河野)美幸, et al. 液胞アミノ酸トランスポーターAvt4N末端領域の機能とその分子機構解明. 日本農芸化学会中四国支部講演会講演要旨集(Web). 2023. 64th
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Patents (56)：
Books (40)：
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Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media 2010
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Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media 2010
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Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media 2010
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Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media 2010
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Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media 2010
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Lectures and oral presentations (11)：
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抗膜タンパク質ウサギ高親和性モノクローナル抗体のエピトープ配列を由来とする新規アフィニティタグの開発
(日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 2015)
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コムギ無細胞系を用いた膜タンパク質間トランス相互作用解析技術
(日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 2015)
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抗膜タンパク質ウサギモノクローナル抗体のエピトープ配列を由来とする新規高親和性ペプチドタグの開発
(日本生化学会大会プログラム・講演要旨集 2014)
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Establishment of robust assay platform based on cell-free protein array to analyze ubiquitin-mediated signal transductions
(The Ubiquitin System: From Basic Science to Drug Discovery (A2) 2014)
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Identification of a Novel E3 Ligase Responsible for Ubiquitination and Subsequent Degradation of Tumor Suppressor Protein PTEN by Wheat Cell-free Based Protein Array
(2013)
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Works (4)：
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小麦無細胞発現系の開発・改良および多検体の発現・可溶性検定
2006 - 2006
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無細胞タンパク質合成法を用いる構造ゲノム科学基盤技術の確立
2005 - 2005
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無細胞タンパク質合成系の保健衛生および畜産分野への応用
2005 - 2005
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小麦胚芽抽出液の改良及び無細胞タンパク質合成の応用技術の開発
2005 - 2005
Education (4)：
- 1992 - 1998 Hiroshima University Graduate School of Science Department of Biological Science
- - 1998 Hiroshima University Graduate School, Division of Natural Science
- 1988 - 1992 Hiroshima University Faculty of Science
- Hiroshima University Faculty of Science
Professional career (1)：
- Ph. D. (Hiroshima University)
Work history (5)：
- 2013/04 - 現在 Ehime University Proteo-Science Center Professor
- 2012/04 - 2013/03 Ehime University Cell-free Science and Technology Center Professor
- 2003/04 - 2012/03 Ehime University Cell-free Science and Technology Center Associate Professor
- 1999/04 - 2003/03 Ehime University Applied Chemistry, Dept. Engineering Assistant Professor
- 1998/10 - 1999/03 Ehime University Applied Chemistry, Dept. Engineering Postdoctoral Fellow
Association Membership(s) (11)：
日本人類遺伝学会
, 植物化学調節学会
, 日本癌学会
, 日本免疫学会
, American Society of Cell Biology
, 日本蛋白質科学会
, 日本ウイルス学会
, 日本農芸化学会
, 日本分子生物学会
, 日本生化学学会
, 日本植物生理学会
