Hiraishi Tomohiro

ヒライシトモヒロ | Hiraishi Tomohiro

Affiliation and department：
Job title： Senior Research Scientist

Research field (1)： Environmental materials/recycling technology

Research keywords (2)：生体高分子 , Biomacromolecule

Research theme for competitive and other funds (8)：

2019 - 2022 Screening of novel beta-peptide hydrolases from metagenomic libraries and their applications for synthesis of functional beta-peptide materials
2015 - 2018 Screening of novel laccases from metagenomic libraries and their applications for synthesis of functional materials
2012 - 2015 Cell surface display of beta-peptide hydrolase and its application for beta-poly(Asp) synthesis
2007 - 2008 Real time analysis of interactions between biopolyester and its degrading enzyme
2006 - 2007 進化工学・遺伝子工学を基軸としたバイオプラスチックの1ステップリサイクルシステム

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Papers (31)：

Hiraishi Tomohiro, Tachibana Koichiro, Asakura Noriyuki, Abe Hideki, Maeda Mizuo. Enhanced expression of a recombinant multicopper oxidase, CueO, from Escherichia coli and its laccase activity towards aromatic substrates. POLYMER DEGRADATION AND STABILITY. 2019. 164. 1-8
Tomohiro Hiraishi. Poly(aspartic acid) (PAA) hydrolases and PAA biodegradation: current knowledge and impact on applications. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2016. 100. 4. 1623-1630
Hiraishi Tomohiro, Abe Hideki, Maeda Mizuo. Substrate stereoselectivity of poly(Asp) hydrolase-1 capable of cleaving beta-amide bonds as revealed by investigation of enzymatic hydrolysis of stereoisomeric beta-tri(Asp)s. AMB EXPRESS. 2015. 5. 1. 118-118
Tan Liu-Tzea, Hiraishi Tomohiro, Sudesh Kumar, Maeda Mizuo. Effects of mutation at position 285 of Ralstonia pickettii T1 poly[(R)-3-hydroxybutyrate] depolymerase on its activities. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2014. 98. 16. 7061-7068
Liu-Tzea Tan, Tomohiro Hiraishi, Kumar Sudesh, Mizuo Maeda. Directed evolution of poly[(R)-3-hydroxybutyrate] depolymerase using cell surface display system: functional importance of asparagine at position 285. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2013. 97. 11. 4859-4871

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MISC (44)：

平石知裕, 前田瑞夫. 非天然型βペプチド:機能・分解・合成新規分解酵素の逆反応を利用した合成プロセスを開発. 化学と生物. 2009. 47. 1. 13-15
Hiraishi, Tomohiro, Maeda, Mizuo, Hirahara, Yoko, Doi, Yoshiharu, Taguchi, Seiichi. Mutational effects of substrate-binding domain of poly[(R)-3- hydoxybutyrate] (PHB) depolymerase on PHB degradation. Polymer Preprints, Japan. 2006. 55. 1
平石知裕, 平原葉子, 田口精一, 土肥義治, 前田瑞夫. ポリヒドロキシブタン酸(PHB)分解におけるPHB分解酵素基質吸着部位への変異導入効果. 高分子学会予稿集(CD-ROM). 2006. 55. 1 Disk1
Tomohiro Hiraishi, Mariko Kajiyama, Kenji Tabata, Ichiro Yamato, Yoshiharu Doi. Genetic analysis and characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1. Biomacromolecules. 2003. 4. 1. 80-86
Seiichi Taguchi, Hirofumi Nakamura, Tomohiro Hiraishi, Ichiro Yamato, Yoshiharu Doi. In vitro evolution of a polyhydroxybutyrate synthase by intragenic suppression-type mutagenesis. Journal of biochemistry. 2002. 131. 6. 801-6

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Education (4)：

Tokyo Institute of Technology School of Bioscience and Biotechnology
Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology
Department of Biotechnology, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
Tokyo Institute of Technology, Graduate Courses of Bioscience and Biotechnology

Professional career (1)：

Work history (5)：

Awards (6)：

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Association Membership(s) (4)：

日本化学会 , 高分子学会 , Society of Polymer Science, Japan , Chemical Society of Japan

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