Rchr
J-GLOBAL ID：200901058521887148
Update date: Feb. 01, 2024
Tsukahara Toshifumi
ツカハラ トシフミ | Tsukahara Toshifumi
Affiliation and department：
Job title：
Professor
Homepage URL (1)：
http://www.jaist.ac.jp/nmcenter/labs/tsukahara-www/index-e.html
Research field (4)：
Molecular biology
, Molecular biochemistry
, Systems genomics
, Genomics
Research keywords (6)：
microarray
, RNA splicing
, restoration of genetic code
, differentiation
, gene expression
, DNA Chip/microarray
Research theme for competitive and other funds (34)：
- 2021 - 2024 遺伝コード修復治療の研究-人為的RNA編集によるRNAの変異修復
- 2018 - 2020 A disease therapy by read-through of nonsense mutations via artificial nucleobase modification
- 2017 - 2020 Development and application of novel in-vivo-RNA restoration method by using enzyme-RNA complexes.
- 2013 - 2017 Establishment of chemical RNA editing method for genetic code resoration therapy
- 2014 - 2016 Development of genetic code restoration by using artificial RNA editing
- 2012 - 2015 Investigation into novel splicing targets of Nucleophosmin
- 2011 - 2014 Development of Photochemical RNA Editing induced by Photothermal Effect
- 2011 - 2012 Development of nucleic acid medicines targeted to generally metastasized cancer cells using collagen derivatives
- 2007 - 2008 遺伝子変異のインビボ修復による疾患治療法の開発
- 2006 - 2007 Improvement of DNA chip system with probe-on-carriers for practical uses
- 2006 - 遺伝子修復による疾患治療の研究
- 1999 - 2004 DNA Chip/microarrayの開発と応用
- 2001 - 2002 神経特異的スプライシング因子、NSSRの機能解析と遺伝子破壊マウスの表現型解析
- 1999 - 2002 Detection of the activation of caspases during neuronal cell death by using antiserum specifically recognizing active form of caspases
- 2000 - 2001 Development of the searching method for SNPs in responsible genes for muscular diseases using the DNA microarray
- 1999 - 2000 神経特異的に発現する新規SRタンパク質、NSSR1および2の機能と生理的役割
- 2000 - Development of Oligonucleotides microarray for SNPs analysis.
- 1999 - 1999 神経特異的に発現する新規SRタンパク質、NSSR1および2の機能と生理的役割
- 1998 - 1999 Function of the interacting protein of SMN, the product of the spinal muscular atrophy gene, and neural specific splicing.
- 1997 - 1998 Molecular cytogenetic study of the myotonic dystrophy by in situ hybrdization (ISH)
- 1996 - 1997 筋ジストロフィーに対するアンチセンスDNAによる化学的治療法の開発
- 1995 - 1996 標的遺伝子組換えによるメロシンM鎖の発現と機能の異常に関する研究
- 1995 - 1996 Assesment of Clinical/Moloeular Genetic Correlation of Muscular Dystrophies.
- 1996 - RNA splicingの制御機構
- 1994 - 1995 Physiological role and effect of (CTG) n of myotonin protein kinase, the product of myotonic dystrophy gene.
- 1995 - Regulation mechanisms of alternative splicing in neuron.
- 1993 - 1994 Basic Research of Gene Therapy using Adenoviral Vector for Muscular Dystrophy
- 1992 - 1994 ジストロフィン関連遺伝子のクローニング
- 1992 - 1993 Molecular Genetic Diagnosis and Prevention of Muscular Dystrophies
- 1989 - 1991 Molecular Pathological Analysis of the Duchenne Muscular Dystrophy Gene Product, Dystrophin.
- 1988 - 1989 Exploration of the mechanism of muscle necrosis in Polymyositis.
- 1987 - 1988 Studies on the mechanism of Cytotoxic T-lymphocyte (CTL)-mediated muscle breakdown
- その他バイオデバイスの開発
- Therapeutic approach for neurological diseases.
Show all
Papers (106)：
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Saifullah, Toshifumi Tsukahara. Integrated analysis of ALK higher expression in human cancer and downregulation in LUAD using RNA molecular scissors. Clinical and Translational Oncology. 2022
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Saifullah, Toshifumi Tsukahara. Integrated analysis of the clinical consequence and associated gene expression of ALK in ALK-positive human cancers. Heliyon. 2022. 8. 7. e09878
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Saifullah, Motohashi N, Tsukahara T, Aoki Y. Development of Therapeutic RNA Manipulation for Muscular Dystrophy. Frontiers in genome editing. 2022. 4. 863651-863651
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Saifullah, Matomo Sakari, Takeshi Suzuki, Seiji Yano, Toshifumi Tsukahara. Effective RNA Knockdown Using CRISPR-Cas13a and Molecular Targeting of the EML4-ALK Transcript in H3122 Lung Cancer Cells. International journal of molecular sciences. 2020. 21. 23
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Tetsuto Tohama, Matomo Sakari, Toshifumi Tsukahara. Development of a Single Construct System for Site-Directed RNA Editing Using MS2-ADAR. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020. 21. 14
ｍore...
MISC (29)：
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TSUKAHARA Toshifumi. Probe-on-Carrier DNA Chip by using Porous Glass. Journal of The Surface Finishing Society of Japan. 2017. 68. 8. 450-454
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長澤 浩, 塚原 俊文. 無機・金属 多孔質ガラスのバイオメディカルへの応用. 未来材料. 2011. 11. 11. 28-33
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M Taniguchi, H Kurahashi, T Okinaga, S Noguchi, T Tsukahara, Nonaka, I, Nishino, I, T Toda. Aberrant neuromuscular junctions and delayed terminal muscle fiber maturation in alpha-dystroglycanopathies. NEUROMUSCULAR DISORDERS. 2004. 14. 8-9. 580-580
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S Noguchi, R Kurokawa, M Fujita, T Tsukahara, A Tsujimoto, Nishino, I. Comprehensive gene expression analysis of the inhibitory effect of myostatin on skeletal myocyte differentiation. NEUROMUSCULAR DISORDERS. 2003. 13. 7-8. 622-622
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T Tsukahara, S Noguchi, M Fujita, R Kurokawa, A Tsujimoto, Nishino, I. Development of a high-sensitive and low-background human muscle cDNA microarray to study muscular dystrophy. AMERICAN JOURNAL OF HUMAN GENETICS. 2002. 71. 4. 527-527
ｍore...
Patents (1)：
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多孔質粒子を用いた反応検出チップ及び該チップの作成方法
Books (4)：
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"New Developments in Alternative Splicing Research"Comprehensive Analyses of Alternative Exons in Neuronally Differentiated P19 cells
Nova Science Publishers, Inc 2013
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多孔質ガラスのバイオメディカルへの応用
未来材料(株)エヌ・ティー・エス 2011
-
新しいDNAチップの科学と応用
講談社 2007 ISBN:9784061538641
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Methods in Molecular Medicine-Neurogenetics: Methods and Protocols,
Humana Press 2003
Lectures and oral presentations (2)：
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Possibility of Genetic Code Restoration by Chemical RNA EditingBIT's
(BIT's Biopharmaceutical Summit 2013 2013)
-
Possibility of Genetic Restoration for a Disease Treatment
(ICMSA02011 2011)
Works (3)：
-
その他
2003 -
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戦略的創造研究推進事業(戦略創造・JST)
2003 -
-
産学官連携イノベーション創出事業
2000 -
Professional career (1)：
- Ph.D. from The University of Tokushima(1987) (The University of Tokushima)
Work history (3)：
- 1990 - 1992 Staff Researcher, National Institute of Neuroscience(1988), Visiting Scientist, Cold Spring Harbor Laboratory(1990-1992), Section Chief, National Institute of Neuroscience(2002), Professor, JAIST(2003)
- 疾病研究第一部
- 国立精神・神経センター神経研究所
Association Membership(s) (4)：
The RNA Society
, 日本RNA学会
, 日本分子生物学会
, 日本生化学会
