Art

J-GLOBAL ID：201702261820950794   Reference number：17A0353096

Construction of chimeric single-chain antibody efficient soluble expression in Escherichia coli and optimization of fermentation conditions

ヒト-鼠嵌抗体の構築とその大腸菌における効率的可溶性発現と発酵条件最適化【JST・京大機械翻訳】

Author (4)： Liu Meng (National Engineering Laboratory for Cereal Fermentation Technology, Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education) ,  Sun Yang (National Engineering Laboratory for Cereal Fermentation Technology, Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education) ,  Yang Yankun (National Engineering Laboratory for Cereal Fermentation Technology, Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education) ,  Bai Zhonghu (National Engineering Laboratory for Cereal Fermentation Technology, Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education)
Material： Shengwu Xue Zazhi
Volume： 33  Issue： 6  Page： 1-6  Publication year： 2016 
JST Material Number： C2987A  ISSN： 2095-1736  Document type： Article
Article type： 原著論文  Country of issue： China (CHN)  Language： CHINESE (ZH)

Abstract/Point： Total RNA was extracted from t...

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Thesaurus term： hybridoma ,  *antibody ,  *optimization ,  chimera ,  light chain ,  vector(genetics) ,  RT-PCR ,  *solubility(property) ,  chimeric antibody ,  DNA ,  *human(primates) ,  heavy chain ,  *solubility
Semi thesaurus term： 一本鎖抗体 ,  遺伝子組換 ,  可変領域 , 【Automatic Indexing@JST】

Author keywords (5)： chimeric antibody ,  scFv ,  soluble expression ,  ELISA ,  optimization of fermentation conditions

JST classification (2)： Production and application of antigens,antibodies,complements  (ED04030W) ,  Biochemistry of antigens,antibodies,complements  (ED04020L)

Terms in the title (9)： ヒト ,  鼠 ,  抗体 ,  構築 ,  大腸菌 ,  可溶性 ,  発現 ,  発酵 ,  最適化