Research theme for competitive and other funds (44)：
2019 - 2024 ミクロオートファジーの作動・制御機構
2019 - 2022 Understanding survival strategy of C1-microorgansms in the phyllosphere from in vitro and in natura experiments
2016 - 2018 Molecular mechanism of micro-organellophagy
2016 - 2018 Production of functional RNA from methanol using high transcription potential of methylotrophic yeasts
2016 - 2018 Molecular mechanism of survival strategy of phyllosphere methylotrophic yeasts exposed to harsh environmental changes and stresses
2014 - 2017 Mechanism analysis of redox imbalance caused by aberrant proteostasis and its recovery using fluorescence protein sensors visualizing redox states.
2014 - 2016 Atg分子群による脂質・オルガネラ動態制御の新しい分子機構
2014 - 2016 Construction of a novel cytosolic protein expression system based on redox regulation in methylotrophic yeast
2013 - 2016 Molecular basis of metabolic physiology of C1-microorganisms and its application in methanol economy
2011 - 2012 Elucidation of signaling pathway with visualization of redox stateduring nitrogen starvation and diseases accompanying intra-cellular reduction
2010 - 2012 Molecular circulation and carbon fixation by C1-microorganism consortia
2010 - 2012 Development of environmental technology based on molecular basis of microbial one-carbon metabolism
2006 - 2011 Proteolysis in the Regulation of Biological Processes
2006 - 2010 Mechanism of intracellular degradation through selective autophagy
2007 - 2008 Studies on cellular function of C1-microorganisms aiming at reduction of green house gas
2006 - 2007 オルガネラとAtg分子の動態解析によるペキソファジー高次機能発現機構の解明
2005 - 2006 ペルオキシソームタンパク質:その新生と分解の決定機構
2004 - 2005 新生膜形成の時空制御とリン脂質結合ドメインの生理生化学機能
2001 - 2005 Molecular and cellular biology and development of useful cellular functions of microorganisms which can utilize future-type natural resources
2003 - 2004 Molecular basis and its application of formaldehyde-fixing reactions in bacteria and Archaea.
2002 - 2004 細菌によるワックスエステル合成のための代謝工学
2000 - 2004 オートファジーを担うApgシステム
2000 - 2002 Construction of new host-vector systems of the production of foreign proteins using a variety of protein targeting signals.
2000 - 2002 Development of bioremediation processes under anoxic conditions using denitrifying bacteria
1999 - 2001 Development of the methanol-inducible super promother and production on of useful heterlolgous proteins
1999 - 2001 Genetic analysis of bacterial formaldehyde-fixing enzyme system and tis application of useful compound production
1999 - 2000 オルガネラ分解の分子機構から異種タンパク生産へのアプローチ
1998 - 1999 脱窒細菌の芳香族化合物分解反応を利用する有用物質生産
1997 - 1999 NEW DIAGNOSTIC ANALYSIS FOR DIABETES MELLITUS USING ENZYMES FROM FILAMENTOUS FUNGI
1997 - 1999 MOLECULAR MECHANISM OF ASSEMBLY OF SINGLE MEMBRANE-BOUND ORGANELLS AND "SORTING ENGINEERING"
1996 - 1998 MOLECULAR ASPECTS OF CONSTRUCTION OF BIOCATALYSTS BASED ON THE CELLULAR FUNCTION OF METHYLOTROPHIC YEASTS
1995 - 1996 Function and sorting of yeast peroxisomal membrane proteins
1995 - 1995 酵母ペルオキシソームのマトリクス酵素及び膜タンパクの遺伝子発現調節
1994 - 1994 メタノール酵母分子育種株によるバイオコンバージョン
1994 - 1994 NAD結合型酸化還元酵素の機能解析とその応用
1993 - 1993 酵母のメタノール制御系を利用した新規異種遺伝子発現系の開発
1992 - 1993 生理活性物質生産のためのバイオコンバージョンプロセスの開発
1991 - 1991 ビタミンK_2及びK_3の微生物合成プロセスの開発
1990 - 1990 メタノール酸化系酵素のギ酸メチル合成への利用とその遺伝子発現制御機構の解明
1990 - 1990 メナキノンの微生物合成と細胞外漏出の機構の解明
1988 - 1988 メタノール酵母酸化系酵素の制御機構の解明とその有用物質生産への利用
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Papers (271)：
Taiki Makizuka, Keisei Sowa, Shiori Katayama, Yuki Kitazumi, Hiroya Yurimoto, Yasuyoshi Sakai, Osamu Shirai. An Enhanced Direct Electron Transfer-type NAD+/NADH Regenerating System Using the Diaphorase Subunit of Formate Dehydrogenase 1. Electrochimica Acta. 2023. 142954-142954
Hiroya Yurimoto, Yasuyoshi Sakai. Interaction between C1-microorganisms and plants: contribution to the global carbon cycle and microbial survival strategies in the phyllosphere. Bioscience, Biotechnology, and Biochemistry. 2022. 87. 1. 1-6
Yasuyoshi Sakai, Hiroya Yurimoto, Seigo Shima. Methane monooxygenases; physiology, biochemistry and structure. Catalysis Science and Technology. 2023. 13. 22. 6342-6354
有馬ゆみ, 中村元春, 中辻拓実, 奥公秀, 白石晃將, 阪井康能. Analysis of KpATG14 required for pexophagy in methylotrophic yeast Komagataella phaffii. 日本農芸化学会大会講演要旨集(Web). 2023. 2023
石垣颯斗, 白石晃將, 光部雅俊, 奥公秀, 阪井康能. Role of ESCRTs in micropexophagy of the methylotrophic yeast Komagataella phaffii. 日本農芸化学会大会講演要旨集(Web). 2023. 2023
光部雅俊, 中辻拓実, 齋藤敬, 奥公秀, 白石晃將, 阪井康能. Regulatory mechanism of autophagy by non-covalent complex Atg12-Atg5 in the yeast Komagataella phaffii. 日本農芸化学会大会講演要旨集(Web). 2023. 2023
井上紘一, 由里本博也, 阪井康能. Analysis of the phosphorylation state of the transcription factor KpMxr1 crucial for the concentration-regulated methanol induction in Komagataella phaffii. 日本農芸化学会大会講演要旨集(Web). 2023. 2023
