Hizukuri Yohei

ヒヅクリヨウヘイ | Hizukuri Yohei

Affiliation and department：

Research field (3)： Bacteriology , Biophysics , Functional biochemistry

Research theme for competitive and other funds (8)：

2022 - 2025 新規構造・基質に基づく細菌S2P膜内切断プロテアーゼの切断制御機構の酵素学的理解
2019 - 2022 細菌III型分泌装置構成因子が受ける新奇な翻訳後多段階プロセシングの分子機構
2018 - 2021 Physiological functions and selective substrate-cleavage mechanism of E. coli intramembrane protease RseP
2017 - 2020 Analysis of regulation mechanism of flagellar type III secretion apparatus mediated by bacterial rhomboid intramembrane protease GlpG
2014 - 2018 Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions

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Papers (21)：

Tatsuhiko Yokoyama, Yutaro Yamagata, Saisei Honna, Shinya Mizuno, Shizuka Katagiri, Rika Oi, Terukazu Nogi, Yohei Hizukuri, Yoshinori Akiyama. S2P intramembrane protease RseP degrades small membrane proteins and suppresses the cytotoxicity of intrinsic toxin HokB. mBio. 2023. 14. 4. e0108623
Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Mizuki Takemoto, Kunio Hirata, Mika Hirose, Rika Oi, Tatsuya Kobayashi, Kenichi Miyoshi, Rie Aruga, et al. Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP. Science advances. 2022. 8. 34. eabp9011
Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Mizuki Takemoto, Kunio Hirata, Mika Hirose, Rika Oi, Tatsuya Kobayashi, Kenichi Miyoshi, Rie Aruga, et al. Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP. bioRxiv. 2022
Risako Tamura-Sakaguchi, Rie Aruga, Mika Hirose, Toru Ekimoto, Takuya Miyake, Yohei Hizukuri, Rika Oi, Mika K Kaneko, Yukinari Kato, Yoshinori Akiyama, et al. Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion. Acta crystallographica. Section D, Structural biology. 2021. 77. Pt 5. 645-662
Tatsuhiko Yokoyama, Tomoya Niinae, Kazuya Tsumagari, Koshi Imami, Yasushi Ishihama, Yohei Hizukuri, Yoshinori Akiyama. The Escherichia coli S2P intramembrane protease RseP regulates ferric citrate uptake by cleaving the sigma factor regulator FecR. The Journal of biological chemistry. 2021. 296. 100673-100673

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MISC (51)：

三宅拓也, 今泉友希, 高貫一徳, 小林達也, 横山達彦, 大井里香, 檜作洋平, 禾晃和, 秋山芳展. 細菌膜内切断プロテアーゼRsePの基質取り込み・切断時におけるダイナミックな構造変化モデル. 日本生化学会大会プログラム・講演要旨集. 2022. 95回. 1T07a-07
檜作洋平, 横山達彦, 三宅拓也, 小林達也, 今泉友希, 高貫一徳, 大井里香, 禾晃和, 秋山芳展. バクテリアの表層変化と生存戦略細菌パーシスター化における膜内切断プロテアーゼRsePの役割生理学的及び構造学的アプローチ. 日本細菌学雑誌. 2022. 77. 1. 19-19
横山達彦, 新苗智也, 津曲和哉, 今見考志, 石濱泰, 檜作洋平, 秋山芳展. A novel physiological function of RseP, an E. coli intramembrane protease: Transcriptional activation of genes involved in iron uptake through the cleavage of FecR. 日本農芸化学会大会講演要旨集(Web). 2021. 2021
檜作洋平, 横山達彦, 秋山芳展. TAシステムを介した細菌の休眠と覚醒:生化学と一細胞解析の融和を目指したアプローチ大腸菌膜内切断プロテアーゼRsePを介したTA systemの制御細胞休眠・覚醒における意義. 日本生化学会大会プログラム・講演要旨集. 2020. 93回. [3S10m-01]
檜作洋平, 横山達彦, 秋山芳展. バクテリアの表層構造の構築と機能大腸菌内膜で働く膜内切断プロテアーゼRsePの新規切断基質探索パーシスター化制御への関与. 日本細菌学雑誌. 2020. 75. 1. 15-15

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Books (4)：

膜内切断プロテアーゼの基質認識・切断機構
一般社団法人日本生物物理学会 2019
Chapter One - Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease. in Methods in Enzymology
Elsevier Inc. 2017
「膜内部での蛋白質分解とストレス応答制御」別冊・医学のあゆみ「レドックスUPDATE -ストレス制御の臨床医学・健康医学」(監修:平家俊男,淀井淳司)
医歯薬出版株式会社 2015
RseP peptidase. in Handbook of Proteolytic Enzymes, 3rd ed. (ed. Rawlings, N. D. and Salvesen, G.)
Elsevier Ltd. 2013

Lectures and oral presentations (46)：

Crystal structure and novel gating mechanistic model to regulate substrate entry and its cleavage of the intramembrane protease RseP
(2023)
Structure-based gating model for substrate accommodation and intramembrane proteolysis of <I>E. coli</I> site 2-protease (S2P), RseP
(IPS2023, 12th General Meeting of the International Proteolysis Society (Singapore) 2023)
膜内切断プロテアーゼRsePのゲーティング機構モデルの検証と基質特異的認識に関する解析
(2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」 2023)
Verification of the substrate recognition and cleavage specificity of RseP, an Escherichia coli S2P family intramembrane protease, by systematic mutational analysis of substrates
(2022)
Verification of the substrate recognition and cleavage specificity of RseP, an Escherichia coli S2P family intramembrane protease, by systematic mutational analysis of substrates
(2022)

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Professional career (1)：

博士(理学) (名古屋大学大学院理学研究科)

Association Membership(s) (3)：

日本分子生物学会 , 日本生化学会 , 日本生物物理学会

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