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J-GLOBAL ID：201702230611368576   Reference number：17A0108523

Generation and characteristic analysis of a stable ST cell line with p53 gene expression knock-down using shRNA-expressing plasmids

P53遺伝子のRNA干渉ベクターの構築とST細胞系における安定発現【JST・京大機械翻訳】

Author (6)： Shen Haiyan (Institude of Animal Health,Guangdong Academy of Agricultural Sciences; Guangdong Open Laboratory of Veterinary Public Health; Guangdong Provincial Key Laboratory of Livestock Disease Prevention) ,  Zhang Chunhong (Institude of Animal Health,Guangdong Academy of Agricultural Sciences; Guangdong Open Laboratory of Veterinary Public Health; Guangdong Provincial Key Laboratory of Livestock Disease Prevention) ,  Liu Zhicheng (Institude of Animal Health,Guangdong Academy of Agricultural Sciences; Guangdong Open Laboratory of Veterinary Public Health; Guangdong Provincial Key Laboratory of Livestock Disease Prevention) ,  Sun Junying (Institude of Animal Health,Guangdong Academy of Agricultural Sciences; Guangdong Open Laboratory of Veterinary Public Health; Guangdong Provincial Key Laboratory of Livestock Disease Prevention) ,  Lu Yu (National Research Center of Veterinary Biologicals Engineering and Technology) ,  Zhang Jianfeng (Institude of Animal Health,Guangdong Academy of Agricultural Sciences; Guangdong Open Laboratory of Veterinary Public Health; Guangdong Provincial Key Laboratory of Livestock Disease Prevention)
Material： Zhongguo Shouyikexue
Volume： 46  Issue： 8  Page： 979-985  Publication year： 2016 
JST Material Number： C3087A  ISSN： 1673-4696  Document type： Article
Article type： 原著論文  Country of issue： China (CHN)  Language： CHINESE (ZH)

Abstract/Point： The study was to establish a s...

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Thesaurus term： transfection ,  *RNA interference ,  plasmid ,  RT-PCR ,  *vector(genetics) ,  cell count ,  gene silencing ,  flow cytometry ,  promoter(genetics) ,  *gene ,  cell proliferation
Semi thesaurus term： 高効率 ,  S期 ,  *細胞株 , 【Automatic Indexing@JST】

Author keywords (4)： p53 gene ,  shRNA vector ,  RNA interference ,  cell line

JST classification (2)： Gene manipulation  (EC02050B) ,  Medical materials  (GA05040H)

Terms in the title (7)： p53遺伝子 ,  RNA干渉 ,  ベクター ,  構築 ,  ST ,  細胞系 ,  安定発現