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J-GLOBAL ID：201702234019366963   整理番号：17A0536919

イネ-1-2Aの原核発現と活性分析【JST・京大機械翻訳】

Prokaryotic Expression and Activity Analysis of Rice Metallothionein Protein OsMT-1-2a

著者 (14件)： Zeng Jiali (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Ouyang Linjuan (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Liu Jialin (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  He Haohua (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Zhu Changlan (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Peng Xiaosong (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  He Xiaopeng (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Fu Junru (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Chen Xiaorong (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Bian Jianmin (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Xu Jie (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Sun Xiaotang (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Zhou Dahu (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production) ,  Hu Lifang (Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Research Center of Super Rice Engineering and Technology, Jiangxi Province, Collaboration Center for Double Cropping Rice Modernization Production)
資料名： Fenzi Zhiwu Yuzhong  (Fenzi Zhiwu Yuzhong)
巻： 14  号： 12  ページ： 3277-3281  発行年： 2016年 
JST資料番号： C2681A  ISSN： 1672-416X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： 中国 (CHN)  言語： 中国語 (ZH)

抄録/ポイント： 本研究の目的は,イネのメタロチオネイン-1-2A遺伝子の原核発現を研究し,活性酸素を除去する能力を研究することである。イネ-1-2A遺伝子を,イネ-1-2Aベクターにクローン化し,大腸菌BL21(DE3)において,可溶性蛋白質の形で発現した。分子量は32KDであり,予想された大きさと一致した。その後、Nie柱法法により目的タンパク質を精製し、WST法により、精製タンパク質は体外にある程度の抗酸化物を有する能力があることが明らかになった。Data from the ScienceChina, LCAS. Translated by JST【JST・京大機械翻訳】

シソーラス用語： クローン ,  *タンパク質 ,  遺伝子 ,  酸化防止 ,  活性酸素 ,  ベクター ,  分子量 ,  メタロチオネイン
準シソーラス用語： 可溶性蛋白質 ,  大腸菌BL21(DE3) , 【Automatic Indexing@JST】

著者キーワード (4件)： Rice ,  Metallothionein ,  Prokaryotic expression ,  Superoxide anion

分類 (3件)： 遺伝子操作  (EC02050B) ,  蛋白質・ペプチド一般  (EB03010N) ,  分子遺伝学一般  (EC02010J)

タイトルに関連する用語 (4件)： イネ ,  発現 ,  活性 ,  分析