文献

J-GLOBAL ID：202002221860065042   整理番号：20A1895934

ヒト多能性幹細胞からの脳幹オルガノイド【JST・京大機械翻訳】

Brainstem Organoids From Human Pluripotent Stem Cells

著者 (42件)： Eura Nobuyuki (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Matsui Takeshi K. (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Matsui Takeshi K. (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Luginbuehl Joachim (Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama,Japan) ,  Matsubayashi Masaya (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Nanaura Hitoki (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Nanaura Hitoki (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Shiota Tomo (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Kinugawa Kaoru (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Iguchi Naohiko (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Kiriyama Takao (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Zheng Canbin (Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, United States) ,  Kouno Tsukasa (Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama,Japan) ,  Lan Yan Jun (Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama,Japan) ,  Kongpracha Pornparn (Laboratory of Biomolecular Dynamics, Department of Collaborative Research, Nara Medical University, Kashihara, Japan) ,  Wiriyasermkul Pattama (Laboratory of Biomolecular Dynamics, Department of Collaborative Research, Nara Medical University, Kashihara, Japan) ,  Sakaguchi Yoshihiko M. (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Nagata Riko (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Komeda Tomoya (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Morikawa Naritaka (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Kitayoshi Fumika (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Jong Miyong (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Kobashigawa Shinko (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Nakanishi Mari (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan) ,  Hasegawa Masatoshi (Department of Radiation Oncology, Nara Medical University, Kashihara, Japan) ,  Saito Yasuhiko (Department of Neurophysiology, Nara Medical University, Kashihara, Japan) ,  Shiromizu Takashi (Department of Integrative Pharmacology, Graduate School of Medicine, Mie University, Tsu, Japan) ,  Nishimura Yuhei (Department of Integrative Pharmacology, Graduate School of Medicine, Mie University, Tsu, Japan) ,  Kasai Takahiko (Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-Chuo Chest Medical Center, Sakai, Japan) ,  Takeda Maiko (Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-Chuo Chest Medical Center, Sakai, Japan) ,  Kobayashi Hiroshi (Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Japan) ,  Inagaki Yusuke (Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Japan) ,  Tanaka Yasuhito (Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Japan) ,  Makinodan Manabu (Department of Psychiatry, Nara Medical University, Kashihara, Japan) ,  Kishimoto Toshifumi (Department of Psychiatry, Nara Medical University, Kashihara, Japan) ,  Kuniyasu Hiroki (Department of Molecular Pathology, Nara Medical University, Kashihara, Japan) ,  Nagamori Shushi (Laboratory of Biomolecular Dynamics, Department of Collaborative Research, Nara Medical University, Kashihara, Japan) ,  Muotri Alysson R. (Department of Pediatrics, University of California, San Diego, San Diego, CA, United States) ,  Muotri Alysson R. (Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, United States) ,  Shin Jay W. (Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama,Japan) ,  Sugie Kazuma (Department of Neurology, Nara Medical University, Kashihara, Japan) ,  Mori Eiichiro (Department of Future Basic Medicine, Nara Medical University, Kashihara, Japan)
資料名： Frontiers in Neuroscience (Web)  (Frontiers in Neuroscience (Web))
巻： 14  ページ： 538  発行年： 2020年 
JST資料番号： U7087A  ISSN： 1662-453X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： スイス (CHE)  言語： 英語 (EN)

抄録/ポイント： 脳幹は脳の後部領域であり,3つの部分,中脳,橋,および延髄から成る。心拍,血圧,および呼吸を制御するのは重要であり,その全ては生命維持機能であり,従って,脳幹の損傷または障害は致死的である。ヒト多能性幹細胞(hPSC)に由来する脳オルガノイドは,ヒト脳発達の経過を再現し,中枢神経系疾患に関する医学研究に有用であると期待されている。しかしながら,既存のオルガノイドモデルは,hPSCがヒト脳発達を再現し,従って脳幹のような脳のさまざまな成分に影響する疾患を完全に解明できない。ここでは,中脳/後脳前駆細胞,ノルアドレナリン作動性およびコリン作動性ニューロン,ドーパミン作動性ニューロンおよび神経冠系統細胞を含む,ヒト脳幹器官(hBSO)を生成する方法を開発した。単細胞RNA配列(scRNA-seq)分析は,プロテオミクスおよび電気生理学からの証拠と共に,これらのオルガノイドにおける細胞集団がヒト脳幹のそれと類似しており,脳幹および効率的な薬物スクリーニングに影響する中枢神経系障害の調査におけるhBSOの使用の可能性を高めることを明らかにした。Copyright 2020 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 血圧 ,  RNA【核酸】 ,  延髄 ,  心拍 ,  中脳 ,  *脳幹 ,  ヒト ,  神経冠 ,  中枢神経系疾患 ,  プロテオミクス ,  *多能性幹細胞
準シソーラス用語： 単一細胞 ,  前駆細胞 ,  ドーパミン作動性ニューロン ,  薬物スクリーニング , 【Automatic Indexing@JST】

著者キーワード (7件)： 脳オルガノイド ,  脳幹 ,  神経堤 ,  中脳 ,  ドーパミン作動性ニューロン ,  ヒト多能性幹細胞 ,  メラノサイト

分類 (2件)： 神経の基礎医学  (GN01020W) ,  細胞生理一般  (EF02010S)

引用文献 (60件)：

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タイトルに関連する用語 (4件)： ヒト ,  多能性幹細胞 ,  脳幹 ,  オルガノイド