特許
J-GLOBAL ID：200903047732951867
大麦β-アミラーゼ、その遺伝子、それを含有する組換ベクター並びに該ベクターを含有する微生物
発明者：
出願人/特許権者：
代理人 (1件)：
吉田 研二 (外2名)
公報種別：公開公報
出願番号（国際出願番号）：特願平6-058119
公開番号（公開出願番号）：特開平6-303983
出願日： 1994年02月18日
公開日（公表日）： 1994年11月01日
要約：
【要約】【構成】 大麦β-アミラーゼの1775塩基からなるcDNAの全塩基配列を決定し、β-アミラーゼの一次構造を明らかにするとともに、該cDNAを発現ベクターに組み込んで、さらに該ベクターを微生物に組み込んで組換えβ-アミラーゼを得た。【効果】 安定した且つ安価で効率の良い組換えβ-アミラーゼの生産が可能となった。
請求項（抜粋）：
下記の式I で表されるアミノ酸配列をコードする大麦β-アミラーゼ遺伝子。式I : Met Glu Val Asn Val Lys Gly Asn Tyr Val Gln Val Tyr Val Met Leu 1 5 10 15 Pro Leu Asp Ala Val Ser Val Asn Asn Arg Phe Glu Lys Gly Asp Glu 20 25 30 Leu Arg Ala Gln Leu Arg Lys Leu Val Glu Ala Gly Val Asp Gly Val 35 40 45 Met Val Asp Val Trp Trp Gly Leu Val Glu Gly Lys Gly Pro Lys Ala 50 55 60 Tyr Asp Trp Ser Ala Tyr Lys Gln Leu Phe Glu Leu Val Gln Lys Ala 65 70 75 80 Gly Leu Lys Leu Gln Ala Ile Met Ser Phe His Gln Cys Gly Gly Asn 85 90 95 Val Gly Asp Ala Val Asn Ile Pro Ile Pro Gln Trp Val Arg Asp Val 100 105 110 Gly Thr Arg Asp Pro Asp Ile Phe Tyr Thr Asp Gly His Gly Thr Arg 115 120 125 Asn Ile Glu Tyr Leu Thr Leu Gly Val Asp Asn Gln Pro Leu Phe His 130 135 140 Gly Arg Ser Ala Val Gln Met Tyr Ala Asp Tyr Met Thr Ser Phe Arg 145 150 155 160 Glu Asn Met Lys Asp Phe Leu Asp Ala Gly Val Ile Val Asp Ile Glu 165 170 175 Val Gly Leu Gly Pro Ala Gly Glu Met Arg Tyr Pro Ser Tyr Pro Gln 180 185 190 Ser His Gly Trp Ser Phe Pro Gly Ile Gly Glu Phe Ile Cys Tyr Asp 195 200 205 Lys Tyr Leu Gln Ala Asp Phe Lys Ala Ala Ala Ala Ala Val Gly His 210 215 220 Pro Glu Trp Glu Phe Pro Asn Asp Ala Gly Gln Tyr Asn Asp Thr Pro 225 230 235 240 Glu Arg Thr Gln Phe Phe Arg Asp Asn Gly Thr Tyr Leu Ser Glu Lys 245 250 255 Gly Arg Phe Phe Leu Ala Trp Tyr Ser Asn Asn Leu Ile Lys His Gly 260 265 270 Asp Arg Ile Leu Asp Glu Ala Asn Lys Val Phe Leu Gly Tyr Lys Val 275 280 285 Gln Leu Ala Ile Lys Ile Ser Gly Ile His Trp Trp Tyr Lys Val Pro 290 295 300 Ser His Ala Ala Glu Leu Thr Ala Gly Tyr Tyr Asn Leu His Asp Arg 305 310 315 320 Asp Gly Tyr Arg Thr Ile Ala Arg Met Leu Lys Arg His Arg Ala Ser 325 330 335 Ile Asn Phe Thr Cys Ala Glu Met Arg Asp Ser Glu Gln Ser Ser Gln 340 345 350 Ala Met Ser Ala Pro Glu Glu Leu Val Gln Gln Val Leu Ser Ala Gly 355 360 365 Trp Arg Glu Gly Leu Asn Val Ala Cys Glu Asn Ala Leu Pro Arg Tyr 370 375 380 Asp Pro Thr Ala Tyr Asn Thr Ile Leu Arg Asn Ala Arg Pro His Gly 390 395 400 405 Ile Asn Gln Ser Gly Pro Pro Glu His Lys Leu Phe Gly Phe Thr Tyr 410 415 420 Leu Arg Leu Ser Asn Gln Leu Val Glu Gly Gln Asn Tyr Val Asn Phe 425 430 435 Lys Thr Phe Val Asp Arg Met His Ala Asn Leu Pro Arg Asp Pro Tyr 440 445 450 Val Asp Pro Met Ala Pro Leu Pro Arg Ser Gly Pro Glu Ile Ser Ile 455 460 465 Glu Met Ile Leu Gln Ala Ala Gln Pro Lys Leu Gln Glu His Thr Asp 470 475 480 485 Leu Pro Val Gly Pro Thr Gly Gly Met Gly Gly Gln Ala Glu Gly Pro 490 495 500 Thr Cys Gly Met Gly Gly Gln Val Lys Gly Pro Thr Gly Gly Met Gly 505 510 515 Gly Gln Ala Glu Asp Pro Thr Ser Gly Met Gly Gly Glu Leu Pro Ala 520 525 530 Thr Met 535
IPC (4件)：
C12N 15/56 ZNA
, C12N 1/21
, C12N 9/26
, C12R 1:19
