間葉系幹細胞からの分化状態を識別する遺伝子群及び分化状態の評価方法 | 特許情報

【課題】骨代謝、骨免疫関連遺伝子変動を簡便に分類、評価することにより、間葉系幹細胞からの細胞の分化状態を評価できる手法を提供する。【解決手段】細胞分化の品質管理に関連する遺伝子群と、それらの発現量の変化を検出するためのオリゴヌクレオチドプローブ。また、当該オリゴヌクレオチドプローブを搭載したDNAマイクロアレイ。さらには、間葉系幹細胞を分化させ、その遺伝子発現パターンの変化を把握することにより、特に、破骨細胞、骨芽細胞または軟骨細胞へ分化した細胞の品質を簡便に評価すること。【選択図】なし

間葉系幹細胞から破骨細胞、骨芽細胞又は軟骨細胞のいずれかへの分化状態を識別するための、以下の遺伝子群から選択される2種以上の遺伝子を含む分化識別用遺伝子群。 aggrecan、acid phosphatase 5 tartrate resistant、activin A receptor type 1、activin A receptor type 1B、activin A receptor type IC、activin receptor IIA、activin receptor IIB、a disintegrin and metallopeptidase domain 17、a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif 5 (aggrecanase-2)、adiponectin C1Q and collagen domain containing、adiponectin receptor 1、alkaline phosphatase(liver/bone/kidney)、androgen receptor、activating transcription factor 4、ATPase H+ transporting lysosomal V0 subunit A1、bone gamma carboxyglutamate protein、bone gamma-carboxyglutamate protein related sequence 1、biglycan、bone morphogenetic protein 2、bone morphogenetic protein 4、bone morphogenetic protein 6、bone morphogenetic protein 7、bone morphogenetic protein receptor type 1A、calcitonin/calcitonin-related polypeptide alpha、calcitonin receptor、calcium-sensing receptor、core binding factor beta、CD44 antigen、cadherin 1、chordin、chloride channel 7、collagen type X alpha 1、collagen type I alpha 1、collagen type II alpha 1、collagen type III alpha 1、collagen type VI alpha 1、collagen type IX alpha 1、cartilage oligomeric matrix protein、colony stimulating factor 1 (macrophage)、colony stimulating factor 1 receptor、chondroitin sulfate N-acetylgalactosaminyltransferase 1、chondroitin sulfate N-acetylgalactosaminyltransferase 2、connective tissue growth factor、catenin (cadherin associated protein) beta 1、cathepsin K、cathepsin L、chemokine (C-X-C motif) receptor 4、cytochrome P450 family 19 subfamily a polypeptide 1、cytochrome P450 family 24 subfamily a polypeptide 1、cytochrome P450 family 27 subfamily b polypeptide 1、dickkopf homolog 1 (Xenopus laevis)、dentin matrix protein 1、ephrin B2、epidermal growth factor、Eph receptor A4、Eph receptor B4、estrogen receptor 1 (alpha)、estrogen receptor 2 (beta)、fibroblast growth factor 23、fibroblast growth factor 10、fibroblast growth factor 17、fibroblast growth factor 18、fibroblast growth factor 2、fibroblast growth factor 4、fibroblast growth factor 8、fibroblast growth factor receptor 1、fibroblast growth factor receptor 2、fibroblast growth factor receptor 3、FMS-like tyrosine kinase 1、fibronectin 1、FBJ osteosarcoma oncogene、FBJ osteosarcoma oncogene B、fos-like antigen 1、fos-like antigen 2、follistatin、frizzled homolog 1 (Drosophila)、gap junction protein alpha 1、GLI-Kruppel family member GLI1、GLI-Kruppel family member GLI2、GLI-Kruppel family member GLI3、gremlin 1、gremlin 2 homolog cysteine knot superfamily (Xenopus laevis)、human immunodeficiency virus type I enhancer binding protein 3、perlecan (heparan sulfate proteoglycan 2)、hyaluronoglucosaminidase 1、hyaluronoglucosaminidase 2、integrin binding sialoprotein、inhibitor of DNA binding 1、inhibitor of DNA binding 2、interferon alpha 1、interferon (alpha and beta) receptor 1、interferon beta 1 fibroblast、interferon gamma、interferon gamma receptor 1、interferon gamma receptor 2、insulin-like growth factor 1、insulin-like growth factor 2、insulin-like growth factor I receptor、insulin-like growth factor 2 receptor、Indian hedgehog、interleukin 17A、interleukin 1 alpha、interleukin 1 beta、interleukin 1 receptor type I、interleukin 6、inhibin beta-A、inhibin beta-B、inhibin beta-C、inhibin beta E、interferon regulatory factor 8、integrin alpha V、integrin beta 3、kinase insert domain protein receptor、60S ribosomal protein L10E、klotho、lymphoid enhancer binding factor 1、leptin、low density lipoprotein receptor-related protein 5、v-maf musculoaponeurotic fibrosarcoma oncogene family protein B (avian)、matrix Gla protein、melanoma inhibitory activity 1、microphthalmia-associated transcription factor、matrix metallopeptidase 13、matrix metallopeptidase 14 (membrane-inserted)、matrix metallopeptidase 1a (interstitial collagenase)、matrix metallopeptidase 1b (interstitial collagenase)、matrix metallopeptidase 2、matrix metallopeptidase 3、matrix metallopeptidase 8、matrix metallopeptidase 9、homeobox msh-like 1、homeobox msh-like 2、myogenic differentiation 1、nuclear factor of activated T cells cytoplasmic calcineurin dependent 1、nuclear factor of activated T cells cytoplasmic calcineurin dependent 2、nuclear factor of activated T cells cytoplasmic calcineurin dependent 3、nuclear factor of kappa light polypeptide gene enhancer in B cells 1 p105、nuclear factor of kappa light polypeptide gene enhancer in B cells 2 p49/p100、noggin、osteoclast associated receptor、platelet derived growth factor alpha、platelet derived growth factor B polypeptide、phosphatase orphan 1、periostin osteoblast specific factor、peroxisome proliferator activated receptor gamma、PR domain containing 1 with ZNF domain、patched homolog 1、patched domain containing 1、prostaglandin-endoperoxide synthase 2、parathyroid hormone、parathyroid hormone 1 receptor、parathyroid hormone-like peptide、v-rel reticuloendotheliosis viral oncogene homolog A (avian)、ras homolog gene family member A、runt related transcription factor 1、runt related transcription factor 2、runt related transcription factor 3、syndecan 3、SFFV proviral integration 1、sonic hedgehog、MAD homolog 1 (Drosophila)、MAD homolog 2 (Drosophila)、MAD homolog 3 (Drosophila)、MAD homolog 4 (Drosophila)、MAD ho molog 5 (Drosophila)、MAD homolog 6 (Drosophila)、MAD homolog 7 (Drosophila)、MAD homolog 9 (Drosophila)、sclerostin、SRY-box containing gene 5、SRY-box containing gene 6、SRY-box containing gene 9、trans-acting transcription factor 3、Sp7 transcription factor 7、secreted acidic cysteine rich glycoprotein、secreted phosphoprotein 1、Rous sarcoma oncogene、transforming growth factor beta 1、transforming growth factor beta 2、transforming growth factor beta 3、transforming growth factor beta receptor I、transforming growth factor beta receptor II、transmembrane 7 superfamily member 4、tumor necrosis factor、tumor necrosis factor receptor superfamily member 11a、tumor necrosis factor receptor superfamily member 1a、tumor necrosis factor receptor superfamily member 1b、tumor necrosis factor (ligand) superfamily member 11、tumor necrosis factor receptor superfamily member 11b (osteoprotegerin)、TNF receptor-associated factor 6、versican、vitamin D receptor、vascular endothelial growth factor A、wingless-related MMTV integration site 3A、wingless-related MMTV integration site 4、wingless-related MMTV integration site 5A、wingless-related MMTV integration site 5B、wingless-related MMTV integration site 6、wingless-related MMTV integration site 7A、wingless-related MMTV integration site 7B、sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain(semaphorin) 4D

C12N 15/09 , C12Q 1/68 , C12M 1/00 , G01N 33/50 , G01N 33/53 , G01N 37/00

C12N15/00 A , C12Q1/68 A , C12N15/00 F , C12M1/00 A , G01N33/50 P , G01N33/53 M , G01N37/00 102

2G045AA40 , 2G045DA13 , 4B024AA11 , 4B024BA80 , 4B024CA09 , 4B024HA12 , 4B029AA27 , 4B029BB20 , 4B029CC03 , 4B029CC08 , 4B029FA15 , 4B029GA08 , 4B063QA01 , 4B063QA05 , 4B063QA19 , 4B063QQ43 , 4B063QQ53 , 4B063QR32 , 4B063QR55 , 4B063QR56 , 4B063QS34 , 4B063QX02

審査官引用 (7件)

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