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J-GLOBAL ID：201702243317031259   整理番号：17A1235139

R2R3-MYB転写因子はポンカンの果皮におけるCrBCH2とCrNCED5の発現を負に調節することによりαおよびβ ブランチカロテノイドの形質転換を抑制する【Powered by NICT】

An R2R3-MYB transcription factor represses the transformation of α- and β-branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

著者 (52件)： Zhu Feng (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zhu Feng (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zhu Feng (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Luo Tao (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Luo Tao (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Luo Tao (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Liu Chaoyang (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Liu Chaoyang (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Liu Chaoyang (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Wang Yang (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Wang Yang (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Wang Yang (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Hongbin (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Hongbin (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Hongbin (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Wei (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Wei (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Yang Wei (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zheng Li (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zheng Li (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zheng Li (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xiao Xue (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xiao Xue (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xiao Xue (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zhang Mingfei (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zhang Mingfei (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zhang Mingfei (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Rangwei (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Rangwei (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Rangwei (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Jianguo (Zhejiang Citrus Research Institute, Taizhou, Zhejiang, 318020, China) ,  Zeng Yunliu (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zeng Yunliu (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Zeng Yunliu (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Juan (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Juan (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Juan (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Qiang (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Qiang (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Xu Qiang (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Guo Wenwu (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Guo Wenwu (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Guo Wenwu (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Larkin Robert M. (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Larkin Robert M. (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Larkin Robert M. (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Deng Xiuxin (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Deng Xiuxin (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Deng Xiuxin (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Cheng Yunjiang (Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Cheng Yunjiang (Key Laboratory of Horticultural Crop Biology and Genetic improvement (Central Region), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China) ,  Cheng Yunjiang (The Institute of Citrus Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, China)
資料名： New Phytologist  (New Phytologist)
巻： 216  号： 1  ページ： 178-192  発行年： 2017年 
JST資料番号： B0319B  ISSN： 0028-646X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： カロチン生成遺伝子の機能は良く実証されているが,それらの発現,特にα およびβ ブランチカロテノイド代謝に関与する遺伝子を調節する機構についてはほとんど知られていない。本研究では,α-およびβ-分岐カロテノイド類の変態を直接調節することをR2R3-MYB転写因子(CrMYB68)はグリーンOugan(MT),Citrus reticulatacv Suavissimaのstay-green変異体を用いて同定した。開発と収穫果実の総合的な分析は,β-カロテンヒドロキシラーゼ2(CrBCH2)と9 cis エポキシカロテノイドジオキシゲナーゼ5(CrNCED5)の発現減少したα-およびβ-カロテンの変換とABAの生合成における遅延の原因であることを示した。それに加えて,これらの遺伝子の発現はCrMYB68の発現と負の相関があった。さらに,電気泳動移動度シフトアッセイ(EMSA)と二重ルシフェラーゼアッセイで,CrMYB68はCrBCH2とCrNCED5を直接調節すると負にできることを示した。さらに,Nicotiana benthamianaの葉を用いた一過性過剰発現実験はCrMYB68もNbBCH2とNbNCED5を負に調節することを示した。トランスジェニック検証の困難さを克服するために,復帰突然変異体におけるカロテノイドとABA(アブシジン酸),及び遺伝子発現の濃度を定量した。これらの実験の結果は,CrMYB68はカロチノイド代謝の重要な調節因子であることをより多くの証拠を提供した。Copyright 2017 Wiley Publishing Japan K.K. All Rights reserved. Translated from English into Japanese by JST.【Powered by NICT】

シソーラス用語： 遺伝子 ,  生合成 ,  *転写因子 ,  復帰突然変異 ,  *ポンカン ,  *カロチノイド ,  代謝 ,  *果皮 ,  遺伝子発現 ,  ミカン属
準シソーラス用語： 調節因子 ,  過剰発現 ,  Nicotiana benthamiana ,  ルシフェラーゼアッセイ ,  カロテン , 【Automatic Indexing@JST】

著者キーワード (6件)： ABA ,  カロチノイド代謝 ,  Citrus reticulata ,  果実の成長及び収穫後 ,  MYB転写因子 ,  転写調節

分類 (1件)： 遺伝子発現  (EC02040Q)

タイトルに関連する用語 (8件)： 転写因子 ,  ポンカン ,  果皮 ,  発現 ,  調節 ,  ブランチ ,  カロテノイド ,  形質転換