文献

J-GLOBAL ID：201702269547538170   整理番号：17A0418331

mir-187前駆体を基にしたsiRNA発現コンストラクトを用いいる,in vitroおよびin vivoでのマウスメラノコルチン-4受容体のRNAi介在性ノックダウン

RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor

著者 (14件)： Kato Minoru (Research Unit/Neuroscience, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Huang Yi-Ying (Laboratory of Chronogenomics, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Matsuo Mina (Experimental Animal Laboratory, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Matsuo Mina (Present address: Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan) ,  Takashina Yoko (Pharmacology Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Sasaki Kazuyo (Pharmacology Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Horai Yasushi (Safety Research Laboratory, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan) ,  Juni Aya (Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Kamijo Shin-Ichi (Experimental Animal Laboratory, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan) ,  Kamijo Shin-Ichi (Present address: Division of Planning of Laboratory Animal Facility, Lifewill Corporation, 2740-12 Shimo-oyamada, Machida, Tokyo 194-0202, Japan) ,  Saigo Kaoru (Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Ui-Tei Kumiko (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan) ,  Tei Hajime (Present address: Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1192, Japan) ,  Tei Hajime (Laboratory of Chronogenomics, Mitsubishi Kagaku Institute of Life Science, 11 Minamiooya, Machida, Tokyo 194-8511, Japan)
資料名： Experimental Animals  (Experimental Animals)
巻： 66  号： 1  ページ： 41-50(J-STAGE)  発行年： 2017年 
JST資料番号： Z0755A  ISSN： 1341-1357  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： 日本 (JPN)  言語： 英語 (EN)

抄録/ポイント： RNA干渉(RNAi)は,トランスジェニックマウスを含む哺乳...

シソーラス用語： アンチセンスRNA ,...
準シソーラス用語： miR-187 ,...

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著者キーワード (4件)： mir-187 ,  mMC4R ,  RNAi ,  Transgenic mice

分類 (5件)： 遺伝学研究法  (EC01020N) ,  遺伝子操作  (EC02050B) ,  遺伝子発現  (EC02040Q) ,  細胞膜の受容体  (EF04030C) ,  中枢神経系  (EJ12030F)

引用文献 (35件)：

• 1. Albarado D.C., McClaine J., Stephens J.M., Mynatt R.L., Ye J., Bannon A.W., Richards W.G., and Butler A.A. 2004. Impaired coordination of nutrient intake and substrate oxidation in melanocortin-4 receptor knockout mice. Endocrinology 145: 243-252.
• 2. Balthasar N., Dalgaard L.T., Lee C.E., Yu J., Funahashi H., Williams T., Ferreira M., Tang V., McGovern R.A., Kenny C.D., Christiansen L.M., Edelstein E., Choi B., Boss O., Aschkenasi C., Zhang C.Y., Mountjoy K., Kishi T., Elmquist J.K., and Lowell B.B. 2005. Divergence of melanocortin pathways in the control of food intake and energy expenditure. Cell 123: 493-505.
• 3. Cai X., Hagedorn C.H., and Cullen B.R. 2004. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10: 1957-1966.
• 4. Clark A.J., Cowper A., Wallace R., Wright G., and Simons J.P. 1992. Rescuing transgene expression by co-integration. Biotechnology (N. Y.) 10: 1450-1454.
• 5. Grimm D., Streetz K.L., Jopling C.L., Storm T.A., Pandey K., Davis C.R., Marion P., Salazar F., and Kay M.A. 2006. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441: 537-541.

タイトルに関連する用語 (8件)： 前駆体 ,  siRNA ,  発現 ,  マウス ,  メラノコルチン ,  受容体 ,  RNAi ,  ノックダウン