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J-GLOBAL ID：201702279635276516   整理番号：17A1457520

中国の野生Vitis pseudoreticulataからの新規なF box/Kelch反復蛋白質,VpEIFP1の過剰発現はチオレドキシンz蛋白質分解を誘導することによるうどんこ病に対する高耐性を付与する【Powered by NICT】

Overexpression of VpEIFP1, a novel F-box/Kelch-repeat protein from wild Chinese Vitis pseudoreticulata, confers higher tolerance to powdery mildew by inducing thioredoxin z proteolysis

著者 (42件)： Wang Jie (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Jie (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Wang Jie (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Yao Wenkong (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Yao Wenkong (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Yao Wenkong (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Lei (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Lei (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Wang Lei (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Ma Fuli (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Ma Fuli (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Ma Fuli (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Tong Weihuo (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Tong Weihuo (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Tong Weihuo (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Chen (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Chen (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Wang Chen (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Bao Rui (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Bao Rui (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Bao Rui (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Jiang Changyue (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Jiang Changyue (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Jiang Changyue (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Yang Yazhou (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Yang Yazhou (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Yang Yazhou (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Zhang Jianxia (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Zhang Jianxia (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Zhang Jianxia (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Xu Yan (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Xu Yan (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Xu Yan (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Xiping (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Xiping (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Wang Xiping (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Zhang Chaohong (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Zhang Chaohong (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Zhang Chaohong (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Yuejin (College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China) ,  Wang Yuejin (Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China) ,  Wang Yuejin (State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China)
資料名： Plant Science  (Plant Science)
巻： 263  ページ： 142-155  発行年： 2017年 
JST資料番号： C0945B  ISSN： 0168-9452  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アイルランド (IRL)  言語： 英語 (EN)

抄録/ポイント： Erysiphe necatorにより誘導されたFボックス蛋白質(VpEIFP1)はVitis pseudoreticulataから単離した,うどんこ病(PM)への自然耐性中国の野生ブドウ種であった。はFボックスドメインと二Kelch反復配列モチーフを含んでいる。発現プロファイルはVpEIFP1はPM感染による転写と翻訳レベルの両方で強く誘導されることを示した。細胞内局在アッセイはVpEIFP1は主に核と細胞質に局在することを示した。VpEIFP1の過剰発現は過酸化水素(H_2O_2)の蓄積を加速し,防御応答に関与するICS2,NPR1とPR1の発現をアップレギュレートし,PM発芽と成長の抑制をもたらした。Fボックス蛋白質として,VpEIFP1は酵母ツーハイブリッド(Y2H)アッセイおよび二分子蛍光相補性(BiFC)アッセイにおいてチオレドキシンz(VpTrxz)と相互作用する。VpEIFP1を過剰発現するトランスジェニックブドウ葉におけるVpTrxz蛋白質の量の低下は,プロテアソーム阻害剤MG132により回復し,VpEIFP1はSCF~VpEIFP1(Skp1キュリンFボックス)E3ユビキチンリガーゼ複合体を通した分解に対してVpTrxzを仲介することを示唆した。VpTrxzのRNA干渉線はPM接種後に増加したH_2O_2蓄積を示した。VpEIFP1はユビキチン/26Sプロテアソーム系を介してVpTrxzの分解を誘導することによりPMへのブドウの防御応答を調節する積極的に提案した。Copyright 2017 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【Powered by NICT】

シソーラス用語： 過酸化水素 ,  *うどんこ病 ,  感染 ,  ユビキチン ,  相互作用 ,  *タンパク質分解 ,  *ブドウ ,  RNA干渉 ,  細胞質 ,  *チオレドキシン ,  発芽
準シソーラス用語： 酵母ツーハイブリッド法 ,  防御応答 ,  Fボックス蛋白質 ,  *過剰発現 , 【Automatic Indexing@JST】

著者キーワード (5件)： Vitis pseudoreticulata ,  レッドグローブを栽培品種Vitis viniferaL. ,  うどんこ病 ,  Fボックス蛋白質 ,  遺伝的形質転換

分類 (2件)： 遺伝子発現  (EC02040Q) ,  酵素生理  (EH02020J)

タイトルに関連する用語 (11件)： 中国 ,  Vitis ,  BOX ,  Kelch反復 ,  蛋白質 ,  過剰発現 ,  チオレドキシン ,  蛋白質分解 ,  誘導 ,  うどんこ病 ,  耐性