文献

J-GLOBAL ID：201802220783256354   整理番号：18A0801055

前駆細胞の関節内注入と併用した外因性間質由来因子-1放出絹足場は骨-靭帯-骨再生を促進する【JST・京大機械翻訳】

Exogenous stromal derived factor-1 releasing silk scaffold combined with intra-articular injection of progenitor cells promotes bone-ligament-bone regeneration

著者 (47件)： Hu Yejun (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Hu Yejun (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Hu Yejun (Department of Sports Medicine, School of Medicine, Zhejiang University, China) ,  Ran Jisheng (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Ran Jisheng (Orthopaedics Research Institute of Zhejiang University, China) ,  Zheng Zefeng (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Jin Zhangchu (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Jin Zhangchu (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Chen Xiao (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Chen Xiao (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Chen Xiao (Department of Sports Medicine, School of Medicine, Zhejiang University, China) ,  Chen Xiao (China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China) ,  Yin Zi (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Yin Zi (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Yin Zi (Department of Sports Medicine, School of Medicine, Zhejiang University, China) ,  Tang Chenqi (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Chen Yangwu (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Huang Jiayun (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Le Huihui (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Yan Ruijian (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Zhu Ting (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Wang Junjuan (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Wang Junjuan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Lin Junxin (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Lin Junxin (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Xu Kan (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Zhou Yiting (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Zhou Yiting (State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China) ,  Zhang Wei (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Zhang Wei (Orthopaedics Research Institute of Zhejiang University, China) ,  Cai Youzhi (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Cai Youzhi (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Dominique Pioletti (Laboratory of Biomechanical Orthopedics, EPFL, Lausanne, Switzerland) ,  Heng Boon Chin (Department of Endodontology, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong) ,  Heng Boon Chin (Department of Biological Sciences, Faculty of Science and Technology, Sunway University, Bandar Sunway, Selangor Darul Ehsan, Malaysia) ,  Chen Weishan (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Chen Weishan (Orthopaedics Research Institute of Zhejiang University, China) ,  Shen Weiliang (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Shen Weiliang (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Shen Weiliang (Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China) ,  Shen Weiliang (Orthopaedics Research Institute of Zhejiang University, China) ,  Shen Weiliang (Department of Sports Medicine, School of Medicine, Zhejiang University, China) ,  Shen Weiliang (China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China) ,  Ouyang Hong-Wei (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China) ,  Ouyang Hong-Wei (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China) ,  Ouyang Hong-Wei (Department of Sports Medicine, School of Medicine, Zhejiang University, China) ,  Ouyang Hong-Wei (China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China)
資料名： Acta Biomaterialia  (Acta Biomaterialia)
巻： 71  ページ： 168-183  発行年： 2018年 
JST資料番号： W3136A  ISSN： 1742-7061  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 前十字靭帯(ACL)は損傷を受けると治癒する最も困難な組織の1つである。靭帯再生と腱-骨接合治癒は,ACL再建の2つの主要な目標である。本研究は,ACL再生のための靭帯由来幹/前駆細胞(LSPCs)の関節内注入と変形性関節症(OA)の長期合併症における改善を組み合わせた,間質細胞由来因子1(SDF-1)放出コラーゲン-絹(CSF)足場の相乗的治療効果を検討することを目的とした。CSF足場の幹細胞動員能と多能性,特にウサギからのLSPCsの腱形成能をin vitroで特性化し,一方,ACL再生とOA改善に対するCSF足場とLSPCsの相乗効果をウサギACL再建モデルを用いて1,3と6か月でin vivoで調べた。CSF足場をACLの代替として使用し,LSPCsをACL再建の7日後に関節空洞に注入した。CSF足場は,機械的性質における剛性の増加により,カプセル化蛋白質に対して7日まで制御された放出パターンを示した。高いIコラーゲンとCXCR4発現を示すLSPCsはSDF-1により誘引され,in vivoで1か月でCSF足場に再配置された。3および6か月後に,LSPCs(CSFL群)と組み合わせたCSF足場は,ACL組織の再生を強化し,骨トンネル治癒を促進した。さらに,OA進行は効率的に妨げられた。著者らの知見は,組織特異的幹細胞を有するCSF足場を動員する幹細胞を用いる新しい戦略を提供し,ACL再生のための有望な解決策である可能性がある。本研究では,靭帯修復のための剛性とSDF-1制御放出能の増加を伴う絹足場を開発した。この進歩した足場移植は,LSPCs(本研究で初めてウサギ靭帯から分離された)の関節内注射と組み合わせて,ACLの腱と骨トンネル部分の両方の再生を促進した。この治療戦略は軟骨変性を改善し,関節線維症の重症度を低下させた。したがって,SDF-1放出絹足場とLSPCs注射を組み合わせることは,臨床におけるACL再生およびOA治療のための治療戦略として示される。ディスプレイを表示した。Copyright 2018 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 剛性 ,  間質細胞 ,  in vitro実験 ,  治療計画 ,  *絹 ,  コラーゲン ,  キャラクタリゼーション ,  治療法 ,  治癒 ,  相乗作用 ,  幹細胞 ,  変形性関節症 ,  *靱帯
準シソーラス用語： ケモカインCXCL12 ,  *骨再生 , 【Automatic Indexing@JST】

著者キーワード (5件)： SDF-1放出絹足場 ,  前十字靭帯 ,  組織工学 ,  再生 ,  靭帯由来幹細胞

分類 (2件)： 運動器系疾患の外科療法  (GG05030O) ,  骨格系  (EJ10030R)

タイトルに関連する用語 (12件)： 前駆細胞 ,  関節内注入 ,  併用 ,  間質 ,  因子 ,  放出 ,  絹 ,  足場 ,  骨 ,  靭帯 ,  骨再生 ,  促進