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J-GLOBAL ID：201802239631496649   整理番号：18A1299857

超高速液体クロマトグラフィー-タンデム質量分析によるラット血漿及びRinger溶液中のIMM-H004及びその活性グルクロニド代謝産物の定量【JST・京大機械翻訳】

Determination of IMM-H004 and its active glucuronide metabolite in rat plasma and Ringer’s solution by ultra-performance liquid chromatography-tandem mass spectrometry

著者 (36件)： Jiang Jianwei (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Jiang Jianwei (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Jiang Jianwei (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Jiang Jianwei (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhang Ziqian (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhang Ziqian (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhang Ziqian (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhang Ziqian (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zou Xiaowen (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zou Xiaowen (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zou Xiaowen (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zou Xiaowen (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Wang Rui (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Wang Rui (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Wang Rui (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Wang Rui (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Bai Jie (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Bai Jie (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Bai Jie (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Bai Jie (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhao Shengyu (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhao Shengyu (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhao Shengyu (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Zhao Shengyu (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Fan Xiaoqing (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Fan Xiaoqing (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Fan Xiaoqing (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Fan Xiaoqing (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Sheng Li (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Sheng Li (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Sheng Li (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Sheng Li (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Li Yan (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Li Yan (Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Li Yan (Beijing Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China) ,  Li Yan (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China)
資料名： Journal of Chromatography B  (Journal of Chromatography B)
巻： 1074-1075  ページ： 16-24  発行年： 2018年 
JST資料番号： W0571A  ISSN： 1570-0232  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： IMM-H004は新しい神経保護剤であり,そのグルクロニド代謝産物IM-H004Gはin vivoおよびin vitroで脳虚血性傷害に対して類似の保護効果を有する。特異的で高感度な超高速液体クロマトグラフィー-タンデム質量分析法を確立し,ラット血漿とリンゲル液中のIM-H004とIM-H004Gの同時定量のために検証した。IMM-H004,IM-H004G及び内部標準プロプラノロールを含む血しょう試料を試料対溶媒比1:2:6(プラズマ:水:アセトニトリル)で直接蛋白質沈殿により調製したが,Ringer溶液試料では蛋白質沈殿は必要としなかった。分離は,0.3mL/分の流速でEclipse Plus C18カラム(2.1×50mm,3.5μm)上に0.5%ギ酸(v/v)を含むメタノール/水の勾配移動相で行った。検出は正イオン多重反応モニタリング(MRM)モードにおける三重四重極質量分析計で操作した。モニターされた遷移は,IM-H004に対して305.1→248.1,IM-H004Gに対して481.3→305.1,およびプロプラノロールに対して260.1→183.1であった。IMM-H004とIM-H004Gの線形範囲は,プラズマ法では5~3000ng/mLと10~3000ng/mLであり,Ringer溶液法では0.5~500ng/mLであった。ラット血漿中の2つの検体の日内および日間精度と精度はすべて7.5%以下であり,Ringer溶液中の検体の日内精度と精度は±14.7%以内であった。明らかなマトリックス効果はなく,検体の回収率は9%以上であった。IM-H004とIM-H004Gは,1つの分析過程の間,安定であった。確立した方法を,IM-H004の単回静脈内投与後のラットにおけるIM-H004とIM-H004Gの血漿薬物動力学と脳微小透析研究に成功裏に適用した。Copyright 2018 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 線形性 ,  同時定量 ,  *ラット ,  in vitro実験 ,  微小透析 ,  薬物動力学 ,  *代謝産物 ,  *血漿 ,  *タンデム質量分析 ,  静脈内投与 ,  カチオン ,  回収率 ,  脂肪族アルコール ,  フェノールエーテル ,  芳香族縮合化合物 ,  アミノアルコール ,  第二アミン ,  第二アルコール
準シソーラス用語： *グルクロニド , 【Automatic Indexing@JST】

著者キーワード (5件)： IMM-H004 ,  グルクロニド代謝物 ,  LC-MS/MS ,  薬物動態 ,  微小透析

分類 (2件)： 有機化合物の各種分析  (CC08034J) ,  生物薬剤学(基礎)  (GY01021U)

物質索引 (2件)： メタノール ,  プロプラノロール

タイトルに関連する用語 (8件)： 高速液体クロマトグラフィー-タンデム質量分析 ,  ラット ,  血漿 ,  溶液 ,  活性 ,  グルクロニド ,  代謝産物 ,  定量