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J-GLOBAL ID：201802250888759741   整理番号：18A1003534

TILD-CRISPRはマウスとヒト細胞における効率的で正確な遺伝子ノックインを可能にする【JST・京大機械翻訳】

Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells

著者 (31件)： Yao Xuan (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Zhang Meiling (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Zhang Meiling (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Wang Xing (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Wang Xing (College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China) ,  Ying Wenqin (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Hu Xinde (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Hu Xinde (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Hu Xinde (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Dai Pengfei (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Meng Feilong (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Shi Linyu (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) ,  Sun Yun (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Sun Yun (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Yao Ning (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Yao Ning (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Zhong Wanxia (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Zhong Wanxia (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Li Yun (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Li Yun (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Wu Keliang (Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China) ,  Wu Keliang (National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China) ,  Wu Keliang (The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250021, China) ,  Li Weiping (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Li Weiping (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Chen Zi-jiang (Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China) ,  Chen Zi-jiang (Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China) ,  Chen Zi-jiang (Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China) ,  Chen Zi-jiang (National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China) ,  Chen Zi-jiang (The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250021, China) ,  Yang Hui (Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China)
資料名： Developmental Cell  (Developmental Cell)
巻： 45  号： 4  ページ： 526-536.e5  発行年： 2018年 
JST資料番号： W1692A  ISSN： 1534-5807  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 相同組換え(HR)を介したknockイン配列の標的化効率は一般的に低い。ここでは,Tild-CRISPR(線形化dsDNA-CRISPRと標的化された統合)と呼ぶ方法を記述し,800-bp相同アームを持つPCR増幅または正確に酵素切断された導入遺伝子ドナーは,マウス接合子にCas9 mRNAと単一ガイドRNAを注入される。既存の標的化戦略と比較して,この方法はマウス胚および脳組織においてはるかに高いknockイン効率を達成した。重要なことに,Tild-CRISPR法は,ヒト胚においてHRに基づく方法より12倍高いknockイン効率をもたらし,in vivoでの遺伝子機能の研究と潜在的遺伝子治療の開発に適している。Copyright 2018 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： ドナー ,  相同組換 ,  酵素 ,  遺伝子療法 ,  ターゲティング ,  アーム ,  PCR法 ,  mRNA ,  *遺伝子 ,  *ヒト
準シソーラス用語： 脳組織 ,  Cas9 ,  導入遺伝子 ,  二本鎖DNA , 【Automatic Indexing@JST】

著者キーワード (6件)： CRISPR/Cas9 ,  Tild-CRISPR ,  ヒト胚 ,  遺伝子編集 ,  ノッキング ,  遺伝子改変マウス

分類 (3件)： 生物学的機能  (EB03040U) ,  発生と分化  (EJ16020W) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (6件)： CRISPR ,  マウス ,  ヒト ,  細胞 ,  遺伝子 ,  ノック