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J-GLOBAL ID：201802264657449472   整理番号：18A2211864

脱分化ブタ網膜色素上皮細胞の間葉-上皮移行を誘導する細胞培養条件【JST・京大機械翻訳】

A cell culture condition that induces the mesenchymal-epithelial transition of dedifferentiated porcine retinal pigment epithelial cells

著者 (37件)： Tian Haibin (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Tian Haibin (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Tian Haibin (Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China) ,  Xu Jing-Ying (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Xu Jing-Ying (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Tian Yu (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Tian Yu (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Cao Yaqi (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Cao Yaqi (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Lian Chunpin (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Lian Chunpin (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Ou Qingjian (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Ou Qingjian (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Wu Binxin (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Wu Binxin (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Jin Caixia (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Jin Caixia (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Gao Furong (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Gao Furong (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Wang Juan (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Wang Juan (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Zhang Jieping (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Zhang Jieping (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Zhang Jingfa (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Zhang Jingfa (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Zhang Jingfa (Department of Physiology and Pharmacology, TUSM, China) ,  Li Weiye (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Li Weiye (Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, USA) ,  Lu Lixia (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Lu Lixia (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Lu Lixia (Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China) ,  Lu Lixia (The Collaborative Innovation Center for Brain Science, Tongji University, Shanghai, China) ,  Xu Guo-Tong (Department of Ophthalmology of Shanghai Tenth Hospital, Tongji Eye Institute, Tongji University School of Medicine (TUSM), Shanghai, China) ,  Xu Guo-Tong (Laboratory of Clinical Visual Science, Department of Regenerative Medicine and Stem Cell Research Center, TUSM, China) ,  Xu Guo-Tong (Department of Physiology and Pharmacology, TUSM, China) ,  Xu Guo-Tong (Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China) ,  Xu Guo-Tong (The Collaborative Innovation Center for Brain Science, Tongji University, Shanghai, China)
資料名： Experimental Eye Research  (Experimental Eye Research)
巻： 177  ページ： 160-172  発行年： 2018年 
JST資料番号： E0658B  ISSN： 0014-4835  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 網膜色素上皮(RPE)細胞の病理学的変化は,加齢黄斑変性(AMD)の発生の主な理由の1つである。従って,培養RPE細胞はin vitroでのAMDの病因を研究するための適切な細胞モデルである。しかし,そのような培養RPE細胞は,細胞の細胞形態と機能の変化をもたらす上皮間葉転換(EMT)を容易に受ける。培養RPE細胞の間充織-上皮転移(MET)を回復し,維持するために,脱分化ブタRPE(prpE)細胞を培養し,4つの条件でそれらの挙動を比較した。1)細胞培養皿において,FBS(CC dish-FBS)を含むDMEM/F12を含む細胞培養皿において,2)は,N2およびB27を含むDMEM/F12を含む細胞培養皿(CC dish-N2B27)および4),N2およびB27を含むDMEM/F12(Petri dish-N2B27)を含む細胞培養皿において。細胞形態と挙動を観察することに加えて,RPE特異的マーカーとEMT関連遺伝子および蛋白質を免疫染色,定量的リアルタイムPCRおよびウエスタンブロット法によって調べた。結果は,脱分化したprpE細胞が,CC dish-FBS,Petri dish-FBSおよびCC dish-N2B27群でEMTを維持し,一方,脱分化したprpE細胞を,Petri dish-N2B27で培養すると,METが誘導されることを示した。このような誘導されたprpE細胞は,RPE特異的マーカーの発現の増加とEMT関連マーカーの減少を伴う多角形形態を示した。同様の結果は,誘導多能性幹細胞由来RPE細胞でも観察された。さらに,これらの脱分化prpE細胞の再分化時に,Petri dish-N2B27はRhoAの活性を低下させ,F-アクチン再配列を誘導し,これはEMT誘導因子であるPDZ結合モチーフ(TAZ)とTAZ標的分子亜鉛フィンガーEボックス結合蛋白質(ZEB1)との転写共活性化を促進した。本研究は,in vitroでのAMDの研究により適切な,上皮化表現型へのprpE細胞の脱分化表現型を逆転させる簡単で信頼できる方法を提供し,他の細胞型のMETが類似のアプローチにより誘導されることを示唆する。Copyright 2019 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *培養 ,  *脱分化 ,  表現型 ,  遺伝子 ,  タンパク質 ,  *間充織 ,  病因 ,  *細胞培養 ,  in vitro実験 ,  *網膜色素上皮 ,  形態 ,  *上皮 ,  免疫染色
準シソーラス用語： Dulbecco変法イーグル培地 ,  細胞形態 , 【Automatic Indexing@JST】

著者キーワード (6件)： RPE ,  加齢黄斑変性 ,  上皮間葉転換 ,  間葉-上皮転換 ,  TAZ ,  ZEB1

分類 (3件)： 眼の基礎医学  (GP01020C) ,  細胞生理一般  (EF02010S) ,  細胞・組織培養法  (EA03040R)

タイトルに関連する用語 (9件)： 分化 ,  ブタ ,  網膜色素上皮細胞 ,  間葉 ,  上皮 ,  移行 ,  誘導 ,  細胞 ,  培養条件