文献

J-GLOBAL ID：201802287685653656   整理番号：18A0471807

磁気活性化細胞選別(MACS)による滑液由来のヒト間葉系幹細胞の単離と特性化【Powered by NICT】

Isolation and characterization of human mesenchymal stem cells derived from synovial fluid by magnetic-activated cell sorting (MACS)

著者 (35件)： Jia Zhaofeng (Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China) ,  Jia Zhaofeng (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Jia Zhaofeng (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Jia Zhaofeng (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Liang Yujie (Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China) ,  Liang Yujie (Shenzhen Key Laboratory for Psychological Healthcare, Shenzhen Institute of Mental Health, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, Guangdong Province, China) ,  Xu Xiao (Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China) ,  Xu Xiao (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Xu Xiao (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Xu Xiao (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Li Xingfu (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Li Xingfu (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Li Xingfu (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Liu Qisong (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Liu Qisong (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Liu Qisong (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Ou Yangkan (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Ou Yangkan (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Ou Yangkan (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Duan Li (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Duan Li (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Duan Li (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Zhu Weimin (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Zhu Weimin (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Zhu Weimin (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Lu Wei (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Lu Wei (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Lu Wei (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Xiong Jianyi (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Xiong Jianyi (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Xiong Jianyi (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China) ,  Wang Daping (Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China) ,  Wang Daping (Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Wang Daping (Department of Orthopedics, Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China) ,  Wang Daping (Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen 518035, Guangdong Province, China)
資料名： Cell Biology International  (Cell Biology International)
巻： 42  号： 3  ページ： 262-271  発行年： 2018年 
JST資料番号： T0950A  ISSN： 1065-6995  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： 間葉系幹細胞(MSC)は組織工学における細胞ベース治療に用いられる細胞の一次源である。MSCは滑液,軟骨組織工学のための便利に使用できることを源で見出されている。しかし,SF MSCの精製と特性化は,文献であまり報告されていた。ここでは,ヒト滑液(hSF MSCs)から誘導されたMSCの単離と培養のための容易なアプローチを概説した。MSC表面マーカーであるCD90を用いた磁気活性化細胞選別(MACS)を用いて精製したhSF MSCに成功した。精製したSF MSCは,培養におけるいくつかの継代後に有意な更新能力を実証した。さらに,MACSソートCD90~+細胞はin vitroで骨芽細胞,脂肪細胞,軟骨細胞に分化できることを示した。添加では,これらの細胞は微量培養で軟骨組織を生成することができることを示した。MACSは滑液からhSF MSCの精製に用いることができる有用なツールであることを示した。軟骨細胞への分化の増殖特性と能力は,これらhSF MSC軟骨修復における応用のための幹細胞の有望な供給源にする。Copyright 2018 Wiley Publishing Japan K.K. All Rights reserved. Translated from English into Japanese by JST.【Powered by NICT】

シソーラス用語： 培養 ,  *単離 ,  *ヒト ,  *キャラクタリゼーション ,  *幹細胞 ,  *細胞分画法 ,  生体組織工学 ,  *間葉系幹細胞 ,  脂肪細胞 ,  骨芽細胞 ,  *滑液 ,  軟骨細胞
準シソーラス用語： 軟骨組織 ,  軟骨組織工学 ,  *MACS , 【Automatic Indexing@JST】

著者キーワード (6件)： CD90 ,  ヒト滑液 ,  磁気活性化細胞選別 ,  間葉系幹細胞 ,  精製 ,  幹細胞単離

分類 (2件)： 細胞生理一般  (EF02010S) ,  医用素材  (GA05040H)

タイトルに関連する用語 (8件)： 磁気 ,  活性化 ,  細胞選別 ,  MACS ,  滑液 ,  ヒト間葉系幹細胞 ,  単離 ,  特性化