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J-GLOBAL ID：201802290342622672   整理番号：18A1041750

ハイスループットスクリーニングはヒト多能性幹細胞からの腎臓器官分化を増強し,自動化多次元表現型を可能にする【JST・京大機械翻訳】

High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

著者 (39件)： Czerniecki Stefan M. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Czerniecki Stefan M. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Czerniecki Stefan M. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Cruz Nelly M. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Cruz Nelly M. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Cruz Nelly M. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Harder Jennifer L. (Department of Internal Medicine, Division of Nephrology, University of Michigan Medical School, Ann Arbor, MI 48109, USA) ,  Menon Rajasree (Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI 48109, USA) ,  Annis James (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Otto Edgar A. (Department of Internal Medicine, Division of Nephrology, University of Michigan Medical School, Ann Arbor, MI 48109, USA) ,  Gulieva Ramila E. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Gulieva Ramila E. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Gulieva Ramila E. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Islas Laura V. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Islas Laura V. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Islas Laura V. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Kim Yong Kyun (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Kim Yong Kyun (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Kim Yong Kyun (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Tran Linh M. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Tran Linh M. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Tran Linh M. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Tran Linh M. (Department of Medicine, Division of Hematology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Martins Timothy J. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Pippin Jeffrey W. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Fu Hongxia (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Fu Hongxia (Department of Medicine, Division of Hematology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Kretzler Matthias (Department of Internal Medicine, Division of Nephrology, University of Michigan Medical School, Ann Arbor, MI 48109, USA) ,  Kretzler Matthias (Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI 48109, USA) ,  Shankland Stuart J. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Himmelfarb Jonathan (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Himmelfarb Jonathan (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Moon Randall T. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Moon Randall T. (Department of Pharmacology, University of Washington School of Medicine and Howard Hughes Medical Institute, Seattle, WA 98109, USA) ,  Paragas Neal (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Freedman Benjamin S. (Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Freedman Benjamin S. (Kidney Research Institute, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Freedman Benjamin S. (Institute for Stem Cell and Regenerative Medicine and Quellos High Throughput Screening Core, University of Washington School of Medicine, Seattle, WA 98109, USA) ,  Freedman Benjamin S. (Department of Pathology, University of Washington School of Medicine, Seattle, WA 98109, USA)
資料名： Cell Stem Cell  (Cell Stem Cell)
巻： 22  号： 6  ページ： 929-940.e4  発行年： 2018年 
JST資料番号： W3106A  ISSN： 1934-5909  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： ヒト多能性幹細胞由来の器官はハイスループットスクリーニング(HTS)のための潜在的に強力なツールであるが,有機物培養の複雑さは小型化と自動化のための重要な挑戦をもたらす。ここでは,ヒト腎臓器官の分化と表現型の増強のための完全に自動化されたHTS適合性プラットフォームを提示する。全体の21日のプロトコルは,めっきから分析への分化まで,液体操作ロボットによって自動的に実行することができるか,または手動のピペットによって代替的に実行することができた。高含量イメージング分析は,器官分化時の用量依存性および閾値効果の両方を明らかにする。免疫蛍光および単一細胞RNA配列決定は,以前に検出されていない壁,間質および部分的に分化した区画を器官内で同定し,血管内皮を大きく拡大する条件を明らかにする。毒性と疾患表現型の化学的調節は,安全性と有効性予測のために定量化できる。この系における遺伝子編集器官におけるスクリーニングは,多嚢胞腎疾患におけるミオシンに対する予想外の役割を明らかにする。このように,HTSフォーマットにおける有機物は,多次元表現型スクリーニングのための魅力的なプラットフォームを確立する。Copyright 2018 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 蛍光 ,  *表現型 ,  *自動化 ,  *高次元 ,  画像処理 ,  *分化 ,  安全性 ,  *スクリーニング ,  プロトコル ,  *ヒト ,  RNA【核酸】 ,  培養 ,  小型化 ,  有機物
準シソーラス用語： *器官 , 【Automatic Indexing@JST】

分類 (2件)： 発生と分化  (EJ16020W) ,  細胞・組織培養法  (EA03040R)

タイトルに関連する用語 (10件)： ハイスループットスクリーニング ,  ヒト ,  多能性幹細胞 ,  腎臓 ,  器官 ,  分化 ,  増強 ,  自動化 ,  多次元 ,  表現型