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J-GLOBAL ID：202002234126557806   整理番号：20A1683350

SARS-CoV-2逆遺伝学は気道における可変感染勾配を明らかにする【JST・京大機械翻訳】

SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract

著者 (47件)： Hou Yixuan J. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Okuda Kenichi (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Edwards Caitlin E. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Martinez David R. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Asakura Takanori (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Dinnon Kenneth H. (Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Kato Takafumi (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Lee Rhianna E. (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Yount Boyd L. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Mascenik Teresa M. (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Chen Gang (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Olivier Kenneth N. (Laboratory of Chronic Airway Infection, Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA) ,  Ghio Andrew (National Health and Environmental Effects Research Laboratory, Environmental Protection Agency, Chapel Hill, NC, USA) ,  Tse Longping V. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Leist Sarah R. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Gralinski Lisa E. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Schaefer Alexandra (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Dang Hong (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Gilmore Rodney (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Nakano Satoko (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Sun Ling (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Fulcher M. Leslie (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Livraghi-Butrico Alessandra (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Nicely Nathan I. (Protein Expression and Purification Core, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Cameron Mark (Department of Population and Quantitative Health Science, Case Western Reserve University, Cleveland, OH, USA) ,  Cameron Cheryl (Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA) ,  Kelvin David J. (Department of Microbiology and Immunology, Canadian Center for Vaccinology, Dalhousie University, Halifax, NS, Canada) ,  Kelvin David J. (Laboratory of Immunology, Shantou University Medical College, Shantou, Guangdong, China) ,  de Silva Aravinda (Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Margolis David M. (Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Margolis David M. (Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Margolis David M. (HIV Cure Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Markmann Alena (Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Bartelt Luther (Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Zumwalt Ross (Department of Pathology, University of New Mexico, Albuquerque, NM, USA) ,  Martinez Fernando J. (Division of Pulmonary and Critical Care Medicine, Joan & Sanford I. Weill Medical College of Cornell University, New York, NY, USA) ,  Salvatore Steven P. (Department of Pathology, Joan & Sanford I. Weill Medical College of Cornell University, New York, NY, USA) ,  Borczuk Alain (Department of Pathology, Joan & Sanford I. Weill Medical College of Cornell University, New York, NY, USA) ,  Tata Purushothama R. (Department of Cell Biology, Regeneration Next Initiative, Duke University Medical Center, Durham, NC, USA) ,  Sontake Vishwaraj (Department of Cell Biology, Regeneration Next Initiative, Duke University Medical Center, Durham, NC, USA) ,  Kimple Adam (Department of Otolaryngology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Jaspers Ilona (Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  O’Neal Wanda K. (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Randell Scott H. (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Boucher Richard C. (Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Baric Ralph S. (Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA) ,  Baric Ralph S. (Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA)
資料名： Cell (Cambridge, Mass.)  (Cell (Cambridge, Mass.))
巻： 182  号： 2  ページ： 429-446.e14  発行年： 2020年 
JST資料番号： A0707B  ISSN： 0092-8674  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： コロナウイルス病2019(COVID-19)の可変臨床スペクトルに対する獲得と原因の様式は不明のままである。重症急性呼吸器症候群コロナウイルス2(SARS-CoV-2)病因とルシフェラーゼレポーターウイルスを探索するためにGFPリポーターウイルスを生成するために逆遺伝学システムを利用して,SARSとCOVID-19患者から採取した血清を限定した交差CoV中和を示した。高感度RNAin situマッピングは,近位(高)対遠位(低)肺上皮培養におけるSARS-CoV-2感染の著しい勾配と平行して,下気道を通して発現低下を伴う鼻における最も高いアンギオテンシン変換酵素2(ACE2)発現を明らかにした。COVID-19剖検肺研究は,気道および肺胞領域でそれぞれ,焦点疾患を同定し,培養データ,SARS-CoV-2感染繊毛および2型肺細胞と一致した。これらの知見はSARS-CoV-2の病因における肺へのその後の吸引媒介ウイルス播種を伴うSARS-CoV-2への鼻感受性を強調する。これらの試薬は,防御免疫,宿主感受性,およびウイルス病原性におけるウイルス-宿主相互作用の研究の基礎を提供する。Copyright 2020 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 感受性 ,  *気道 ,  血清 ,  上皮 ,  肺胞 ,  ルシフェラーゼ ,  スペクトル ,  繊毛 ,  剖検 ,  高感度 ,  中性化 ,  病因
準シソーラス用語： コロナウイルス ,  *SARSウイルス ,  *逆遺伝学 , 【Automatic Indexing@JST】

著者キーワード (10件)： SARS-CoV-2 ,  COVID-19 ,  逆遺伝学 ,  感染性クローン ,  鼻感染症 ,  中和アッセイ ,  一次細胞 ,  レポーターウイルス ,  呼吸向性 ,  ACE2

分類 (3件)： 生物学的機能  (EB03040U) ,  分子遺伝学一般  (EC02010J) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (5件)： SARS-CoV-2 ,  逆遺伝学 ,  気道 ,  感染 ,  勾配