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J-GLOBAL ID：202002268622544934   整理番号：20A0653069

マウス胚線維芽細胞から線維軟骨細胞への化学的再プログラミングの高分解能解剖【JST・京大機械翻訳】

High-Resolution Dissection of Chemical Reprogramming from Mouse Embryonic Fibroblasts into Fibrocartilaginous Cells

著者 (43件)： Chen Yishan (Department of Orthopaedic Surgery, Second Affiliated Hospital and Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Chen Yishan (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Chen Yishan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Wu Bingbing (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Wu Bingbing (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Lin Junxin (Department of Orthopaedic Surgery, Second Affiliated Hospital and Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Lin Junxin (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Lin Junxin (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Yu Dongsheng (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Yu Dongsheng (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Du Xiaotian (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Du Xiaotian (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Sheng Zixuan (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Sheng Zixuan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Yu Yeke (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Yu Yeke (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  An Chengrui (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  An Chengrui (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Xiaoan (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Xiaoan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Li Qikai (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Li Qikai (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhu Shouan (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhu Shouan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Sun Heng (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Sun Heng (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Xianzhu (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Xianzhu (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Shufang (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Shufang (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhang Shufang (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou 310058, China) ,  Zhou Jing (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Zhou Jing (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Bunpetch Varitsara (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Bunpetch Varitsara (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  El-Hashash Ahmed (Department of Orthopaedic Surgery, Second Affiliated Hospital and Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ji Junfeng (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ji Junfeng (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ouyang Hongwei (Department of Orthopaedic Surgery, Second Affiliated Hospital and Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ouyang Hongwei (Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ouyang Hongwei (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ouyang Hongwei (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China) ,  Ouyang Hongwei (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou 310058, China)
資料名： Stem Cell Reports  (Stem Cell Reports)
巻： 14  号： 3  ページ： 478-492  発行年： 2020年 
JST資料番号： W3132A  ISSN： 2213-6711  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 関節軟骨損傷と疼痛の原因となる変性およびクオリティオブライフの損失は,ますます高齢化された集団にとって深刻な問題になっている。成人のヒト軟骨細胞の自己再生が乏しいため,代替機能細胞源が必要である。小分子による直接再プログラミングは,軟骨細胞を発生させるための癌遺伝子フリーで費用対効果の高いアプローチを提供する可能性があるが,まだ研究されていない。ここでは,化学カクテル,VCRTc(バルプロ酸,CHIR98014,Repsox,TTNPBおよびセレコキシブ)を用いた3Dシステムを用いて,マウス胚線維芽細胞をPRG4+軟骨細胞に直接再プログラムした。単一細胞トランスクリプトームを用いて,初期再プログラミングにおける線維芽細胞の特徴と軟骨形成経路の活性化の阻害,および軟骨発生に似た中間細胞過程を明らかにした。欠損関節表面での化学誘導軟骨細胞のin vivo移植は,欠損治癒を促進し,機械的機能損失の63.4%を救済した。著者らのアプローチは線維芽細胞を機能性軟骨細胞に直接変換し,将来の軟骨再生のための潜在的な薬理学的戦略への洞察も提供する。Copyright 2020 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 化学物質 ,  ヒト ,  *軟骨 ,  *線維芽細胞 ,  トランスクリプトーム ,  *軟骨細胞 ,  薬理学 ,  クオリティオブライフ ,  癌遺伝子 ,  疼痛 ,  *高分解能 ,  三次元 ,  小分子 ,  *解剖
準シソーラス用語： *リプログラミング , 【Automatic Indexing@JST】

著者キーワード (4件)： ケミカルリプログラミング ,  単一細胞分析 ,  軟骨再生 ,  創薬

分類 (3件)： 運動器系の基礎医学  (GG01020B) ,  骨格系  (EJ10030R) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (6件)： マウス胚線維芽細胞 ,  軟骨細胞 ,  化学 ,  再プログラミング ,  高分解能 ,  解剖