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J-GLOBAL ID：202002271727778711   整理番号：20A2627477

SARS-CoV-2感染は細胞ヘパラン硫酸とACE2に依存する【JST・京大機械翻訳】

SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2

著者 (52件)： Clausen Thomas Mandel (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Clausen Thomas Mandel (Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark) ,  Clausen Thomas Mandel (Department of Infectious Disease, Copenhagen University Hospital, 2200 Copenhagen, Denmark) ,  Sandoval Daniel R. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Spliid Charlotte B. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Spliid Charlotte B. (Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark) ,  Spliid Charlotte B. (Department of Infectious Disease, Copenhagen University Hospital, 2200 Copenhagen, Denmark) ,  Pihl Jessica (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Pihl Jessica (Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark) ,  Pihl Jessica (Department of Infectious Disease, Copenhagen University Hospital, 2200 Copenhagen, Denmark) ,  Perrett Hailee R. (Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA) ,  Painter Chelsea D. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Painter Chelsea D. (Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California, USA) ,  Narayanan Anoop (Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA) ,  Majowicz Sydney A. (Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA) ,  Kwong Elizabeth M. (Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA) ,  McVicar Rachael N. (Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA) ,  Thacker Bryan E. (TEGA Therapeutics, Inc., 3550 General Atomics Court, G02-102, San Diego, CA 92121, USA) ,  Glass Charles A. (TEGA Therapeutics, Inc., 3550 General Atomics Court, G02-102, San Diego, CA 92121, USA) ,  Yang Zhang (Copenhagen Center for Glycomics, Department of Molecular and Cellular Medicine, University of Copenhagen, 2200 Copenhagen, Denmark) ,  Torres Jonathan L. (Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA) ,  Golden Gregory J. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Golden Gregory J. (Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California, USA) ,  Bartels Phillip L. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Bartels Phillip L. (Glycobiology Research and Training Center, University of California, San Diego, La Jolla, CA 92093, USA) ,  Porell Ryan N. (Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA) ,  Garretson Aaron F. (Department of Medicine, University of California, San Diego, La Jolla, CA 92037, USA) ,  Laubach Logan (Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA) ,  Feldman Jared (Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA) ,  Yin Xin (Immunity and Pathogenesis Program, Infectious and Inflammatory Disease Center, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA) ,  Pu Yuan (Immunity and Pathogenesis Program, Infectious and Inflammatory Disease Center, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA) ,  Hauser Blake M. (Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA) ,  Caradonna Timothy M. (Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA) ,  Kellman Benjamin P. (Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA) ,  Kellman Benjamin P. (Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, CA 92093, USA) ,  Kellman Benjamin P. (Bioinformatics and Systems Biology Program, University of California, San Diego, La Jolla, CA 92093, USA) ,  Martino Cameron (Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA) ,  Martino Cameron (Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, CA 92093, USA) ,  Gordts Philip L.S.M. (Department of Medicine, University of California, San Diego, La Jolla, CA 92037, USA) ,  Gordts Philip L.S.M. (Glycobiology Research and Training Center, University of California, San Diego, La Jolla, CA 92093, USA) ,  Chanda Sumit K. (Immunity and Pathogenesis Program, Infectious and Inflammatory Disease Center, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA) ,  Schmidt Aaron G. (Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA) ,  Schmidt Aaron G. (Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA) ,  Godula Kamil (Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA) ,  Godula Kamil (Glycobiology Research and Training Center, University of California, San Diego, La Jolla, CA 92093, USA) ,  Leibel Sandra L. (Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, CA 92093, USA) ,  Jose Joyce (Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA) ,  Corbett Kevin D. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Ward Andrew B. (Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA) ,  Carlin Aaron F. (Department of Medicine, University of California, San Diego, La Jolla, CA 92037, USA) ,  Esko Jeffrey D. (Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA) ,  Esko Jeffrey D. (Glycobiology Research and Training Center, University of California, San Diego, La Jolla, CA 92093, USA)
資料名： Cell (Cambridge, Mass.)  (Cell (Cambridge, Mass.))
巻： 183  号： 4  ページ： 1043-1057.e15  発行年： 2020年 
JST資料番号： A0707B  ISSN： 0092-8674  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： SARS-CoV-2スパイク蛋白質はその受容体結合ドメイン(RBD)を介して細胞ヘパラン硫酸及びアンギオテンシン変換酵素2(ACE2)と相互作用することを示した。ドッキング研究により,ACE2結合部位に隣接するヘパリン/ヘパリン硫酸塩結合部位を示唆した。ACE2とヘパリンの両方は,in vitroでスパイク蛋白質に独立して結合し,足場としてヘパリンを用いて3成分複合体を生成することができる。スパイク蛋白質のElectron顕微鏡写真は,ヘパリンがACE2に結合するRBDの開放立体配座を増強することを示唆する。細胞上で,スパイク蛋白質結合はヘパラン硫酸とACE2の両方に依存した。未分画ヘパリン,非抗凝固ヘパリン,ヘパリンリアーゼおよび肺ヘパラン硫酸は,偽型ウイルスおよび真正SARS-CoV-2ウイルスによるスパイク蛋白質結合および/または感染を強力に阻止した。ウイルス付着と感染がACE2への結合のヘパラン硫酸依存性増強を含むモデルを示唆した。ヘパラン硫酸の操作または外因性ヘパリンによるウイルス接着の阻害は,新しい治療機会を示す。Copyright 2020 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 凝固 ,  接着 ,  相互作用 ,  付着 ,  立体配座 ,  ドッキング ,  受容体 ,  ヘパリン ,  結合部位 ,  複合体 ,  ヘパリナーゼ ,  *ヘパラン硫酸 ,  in vitro実験
準シソーラス用語： *重症急性呼吸器症候群 ,  スパイク蛋白質 , 【Automatic Indexing@JST】

著者キーワード (9件)： COVID-19 ,  SARS-CoV-2 ,  コロナウイルス ,  スパイク蛋白質 ,  ヘパリン ,  ヘパラン硫酸 ,  偽型ウイルス ,  ヘパラン硫酸結合蛋白質 ,  肺上皮細胞

分類 (2件)： 生物学的機能  (EB03040U) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (5件)： SARS-CoV-2 ,  感染 ,  細胞 ,  ヘパラン硫酸 ,  ACE2