文献

J-GLOBAL ID：202002283986841668   整理番号：20A0481796

ステルス・ナノビークルを形成するフェリチンの表面パシル化はカプセル化エリプチシンのin vivo治療性能を増強する【JST・京大機械翻訳】

Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine

著者 (39件)： Tesarova Barbora (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Tesarova Barbora (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Dostalova Simona (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Dostalova Simona (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Smidova Veronika (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Smidova Veronika (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Goliasova Zita (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Goliasova Zita (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Skubalova Zuzana (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Skubalova Zuzana (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Michalkova Hana (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Michalkova Hana (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Hynek David (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Hynek David (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Michalek Petr (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Michalek Petr (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Polanska Hana (Department of Pathological Physiology and Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 753/5, CZ,625 00, Brno, Czech Republic) ,  Vaculovicova Marketa (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Vaculovicova Marketa (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic) ,  Hacek Jaromir (Department of Pathology and Molecular Medicine, Second Faculty of Medicine, Charles University and University Hospital Motol, V Uvalu 84, CZ, 150 06, Prague 5, Czech Republic) ,  Eckschlager Tomas (Department of Paediatric Haematology and Oncology, Charles University and University Hospital Motol, V Uvalu 84/1, CZ, 150 06, Prague 5, Czech Republic) ,  Stiborova Marie (Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030/8, CZ,128 43, Prague 2, Czech Republic) ,  Pires Ana S. (CNC.IBILI Consortium/Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Pires Ana S. (Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal) ,  Neves Ana R.M. (Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal) ,  Abrantes Ana M. (CNC.IBILI Consortium/Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Abrantes Ana M. (Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal) ,  Rodrigues Tiago (Institute of Physiology and Coimbra Institute for Clinical and Biomedical Research (iCBR), CNC.IBILI - Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Matafome Paulo (Institute of Physiology and Coimbra Institute for Clinical and Biomedical Research (iCBR), CNC.IBILI - Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Botelho Maria F. (CNC.IBILI Consortium/Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Botelho Maria F. (Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal) ,  Teixeira Paulo (CNC.IBILI Consortium/Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Teixeira Paulo (Pathology Department, Coimbra Hospital and Universitary Centre, Praceta Prof. Mota Pinto, 3000-075 Coimbra, Portugal) ,  Teixeira Paulo (Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Department Biomedical Laboratory Sciences, Rua 5 de Outubro, 3046-854, Coimbra, Portugal) ,  Mendes Fernando (CNC.IBILI Consortium/Centre for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548, Coimbra, Portugal) ,  Mendes Fernando (Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal) ,  Mendes Fernando (Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Department Biomedical Laboratory Sciences, Rua 5 de Outubro, 3046-854, Coimbra, Portugal) ,  Heger Zbynek (Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ, 613 00, Brno, Czech Republic) ,  Heger Zbynek (Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ, 612 00, Brno, Czech Republic)
資料名： Applied Materials Today  (Applied Materials Today)
巻： 18  ページ： Null  発行年： 2020年 
JST資料番号： W3082A  ISSN： 2352-9407  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 表面機能化は,血流における生体適合性,選択性および循環を改善することにより,薬物送達媒体の性能に実質的に影響する。ここでは,フェリチン(FRT)に基づくナノキャリア(FRTEliと呼ばれる)の内部空洞にカプセル化された非常に強力な細胞静止アルカロイドエリプチシン(Elli)のin vitroおよびin vivo挙動の研究を提示する。加えて,FRTEli表面は3つの異なる分子被覆で機能化された:アミノ酸プロリン(P),アラニン(A)およびセリン(S)(それぞれPAS-10-FRTEliまたはPAS-20-FRTEliを形成する)またはポリエチレングリコール(PEG-FRTEli)から成る配列を持つ2つのタイプの保護PASペプチド(10または20残基長)。FRTの3つの全ての表面改質は,カーゴの早期累積放出なしで,Elliの十分なカプセル化効率を処理した。注目すべきことに,全ての試験した表面修飾はin vitro生体適合性に対して有益な効果を示した。PAS-10-FRTEliは,PAS-20-FRTEli,PEG-FRTEliまたは非修飾FRTEliと比較して,マクロファージによる取り込みを著しく低下させた。PAS-10-FRTEliの例外的性質は,蛋白質コロナの形成,取り込み効率または細胞毒性の選択性のスクリーニングを含むin vitro分析のアレイにより検証された。3重陰性乳癌(MDA-MB-231)異種移植片を有するマウス前臨床モデルにおいて,遊離ElliまたはFRTEliと比較して,PAS-10-FRTEliは腫瘍組織内のEllの蓄積を増強したが,Elllのオフ標的組織への取り込みを妨げた。注目すべきことに,PAS-10-FRTEliはin vivo補体(C3)活性化と蛋白質コロナ形成を減少させた。まとめると,in vivo結果はPAS-10-FRTEliが臨床設定への翻訳のための有望なステルスプラットフォームであることを示す。Copyright 2020 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 補体 ,  媒質 ,  細胞毒性 ,  生体適合性 ,  in vitro実験 ,  貨物 ,  ドラッグデリバリーシステム ,  乳房腫瘍 ,  血流 ,  被覆 ,  マクロファージ ,  ペプチド ,  *フェリチン ,  表面改質
準シソーラス用語： ナノキャリア , 【Automatic Indexing@JST】

著者キーワード (6件)： 生体適合 ,  乳癌 ,  癌治療 ,  フェリチン ,  パシル化 ,  PEG化

分類 (4件)： 強化プラスチックの成形  (YH04060J) ,  無機化合物一般及び元素  (CD01010D) ,  金属薄膜  (BK14030T) ,  機械的性質  (YH02022L)

タイトルに関連する用語 (6件)： フェリチン ,  パシル ,  カプセル化 ,  エリプチシン ,  治療 ,  増強