文献

J-GLOBAL ID：202002285903639086   整理番号：20A0501135

移植部位は網膜変性に対する脂肪由来幹細胞に基づく治療の転帰に影響する【JST・京大機械翻訳】

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

著者 (38件)： Hu Chengyu (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Hu Chengyu (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  La Huanzhi (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  La Huanzhi (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Wei Xuancheng (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Wei Xuancheng (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Zhou Yue (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Zhou Yue (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Ou Qingjian (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Ou Qingjian (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Chen Zhiyang (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Chen Zhiyang (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Zhu Xiaoman (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Zhu Xiaoman (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Xu Jing-Ying (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Xu Jing-Ying (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Jin Caixia (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Jin Caixia (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Gao Furong (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Gao Furong (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Wang Juan (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Wang Juan (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Zhang Jingfa (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Zhang Jingfa (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Zhang Jieping (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Zhang Jieping (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Zhang Jieping (Department of Physiology and Pharmacology, TUSM, Shanghai, China) ,  Lu Lixia (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Lu Lixia (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Lu Lixia (Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China) ,  Lu Lixia (The Collaborative Innovation Center for Brain Science, Tongji University, Shanghai, China) ,  Xu Guo-Tong (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Xu Guo-Tong (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China) ,  Xu Guo-Tong (Department of Physiology and Pharmacology, TUSM, Shanghai, China) ,  Xu Guo-Tong (Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China) ,  Xu Guo-Tong (The Collaborative Innovation Center for Brain Science, Tongji University, Shanghai, China) ,  Tian Haibin (Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China) ,  Tian Haibin (Laboratory of Clinical Visual Science, Department of Regenerative Medicine, Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China)
資料名： Stem Cells International (Web)  (Stem Cells International (Web))
巻： 2020  ページ： Null  発行年： 2020年 
JST資料番号： U7828A  ISSN： 1687-966X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： 脂肪由来幹細胞(ASCs)は,動物モデルにおいて網膜下空間に移植された後,網膜変性疾患(RDD)に対して強い保護効果を示した。最近,ASCsを含む幹細胞の硝子体内移植によりRDDを治療するためのいくつかの臨床試験が行われている。しかし,臨床試験の結果は満足できなかった。移植部位がRDDに対する幹細胞に基づく治療の結果を変化させるかどうかを調べるため,ラットASCs(rASCs)を分離し,それらを緑色蛍光蛋白質で標識した。自己rASCsをヨウ素酸ナトリウム(SI)により誘導されたラットRDDモデルにおいて硝子体チャンバーまたは網膜下空間に移植した。電気応答をERGにより記録した。網膜の解剖学的構造は,移植後週1,2および4でラット眼の凍結切片で観察された。神経網膜アポトーシスと網膜上膜(ERM-)様構造形成を免疫染色により調べた。rASCsの硝子体内移植は,神経網膜のロゼット形成とアポトーシスを減少させたが,消失した電気応答をもたらした。しかし,網膜下空間に移植されたrASCsは,電気応答の部分的回復とロゼット形成の減少を含むSIによって引き起こされた損傷から網膜を保護した。硝子体内に移植したrASCsは膜を形成し,注入部位で網膜の折り畳みをもたらした。ミュラー細胞,網膜色素上皮細胞およびミクログリア細胞は網膜からrASC形成膜へ移動し,その後ERM様構造を形成した。さらに,硝子体液はrasC移動を促進し,rASC条件培地はin vitro研究により示されるようにミュラー細胞移動を増強した。これらのデータは,硝子体チャンバーがRDDに対するASCに基づく治療のための良好な移植部位ではないことを示唆し,臨床試験でRDDを治療するために幹細胞を硝子体チャンバーに移植する前に慎重な決定を行うべきであることを示唆した。Copyright 2020 Chengyu Hu et al. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 臨床試験 ,  *網膜変性 ,  *幹細胞 ,  ヨウ素酸ナトリウム ,  凍結切片法 ,  アポトーシス ,  折畳み ,  ロゼット形成 ,  硝子体 ,  免疫染色 ,  *網膜 ,  in vitro実験 ,  ガラス ,  ラット ,  緑色蛍光タンパク質

分類 (1件)： 眼の基礎医学  (GP01020C)

引用文献 (48件)：

• A. Gheorghe, L. Mahdi, O. Musat, "Age-related macular degeneration," Romanian Journal of Ophthalmology, vol. 59, no. 2, pp. 74-77, 2015.
• B. P. Hafler, "Clinical progress in inherited retinal degenerations: gene therapy clinical trials and advances in genetic sequencing," Retina, vol. 37, no. 3, pp. 417-423, 2017.
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• P. A. Zuk, M. Zhu, H. Mizuno, J. Huang, J. W. Futrell, A. J. Katz, P. Benhaim, H. P. Lorenz, M. H. Hedrick, "Multilineage cells from human adipose tissue: implications for cell-based therapies," Tissue Engineering, vol. 7, no. 2, pp. 211-228, 2001.
• H. Salehi, N. Amirpour, A. Niapour, S. Razavi, "An overview of neural differentiation potential of human adipose derived stem cells," Stem Cell Reviews, vol. 12, no. 1, pp. 26-41, 2016.

タイトルに関連する用語 (5件)： 移植 ,  網膜変性 ,  脂肪由来幹細胞 ,  治療 ,  転帰