文献

J-GLOBAL ID：202102243153966169   整理番号：21A0272672

培養ウシ副腎髄質細胞におけるニコチン性アセチルコリン受容体イオンチャンネルの調節によるアセチルコリン誘発カテコールアミン合成および分泌に対するShenmai注射の抑制効果【JST・京大機械翻訳】

The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells

著者 (36件)： Zhang Xiting (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Zhang Xiting (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Zhang Xiting (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Zhang Xiting (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Li Lin (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Li Lin (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Li Lin (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Li Lin (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Yi (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Yi (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Yi (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Wang Yi (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Mao Haoping (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Mao Haoping (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Mao Haoping (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Mao Haoping (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Chai Lijuan (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Chai Lijuan (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Chai Lijuan (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Chai Lijuan (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Miao Lin (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Miao Lin (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Miao Lin (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Miao Lin (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Shuang (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Shuang (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Wang Shuang (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Wang Shuang (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Gao Xiumei (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Gao Xiumei (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Gao Xiumei (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Gao Xiumei (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Zhang Han (Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Zhang Han (State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China) ,  Zhang Han (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 301617, China) ,  Zhang Han (Laboratory of Pharmacology of TCM Formulae Co-Constructed by the Province-Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China)
資料名： Evidence-Based Complementary and Alternative Medicine (Web)  (Evidence-Based Complementary and Alternative Medicine (Web))
巻： 2020  ページ： Null  発行年： 2020年 
JST資料番号： U7703A  ISSN： 1741-427X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： Shenmai注射(SMI)は,中国の心血管疾患の治療に広く使用されている。心血管疾患はしばしば過剰なカテコールアミン(CA)分泌に関連する。ここでは,培養ウシ副腎髄細胞におけるCA分泌と合成に対するSMIの影響を報告する。SMIは300μMアセチルコリン(ACh)により誘導されるCA分泌を有意に低下させることを見出した。SMI(10μL/mL)とACh受容体αサブユニット阻害剤,HEX(α3)またはDhβE(α4β2)のどちらかの処理は,更なる阻害を生じず,SMIがα3とα4β2チャンネルを介し役割をすることを示した。さらに,SMIは,Ser19およびSer40でのTHのリン酸化を阻害することにより,AChにより誘導されたチロシンヒドロキシラーゼ(TH)活性を低下させた。THはCa2+/カルモジュリン依存性蛋白質キナーゼII(CaMキナーゼII)によりSer19でリン酸化され,蛋白質キナーゼA(PKA)によりSer40でリン酸化される。CaMキナーゼIIとPKAのアンタゴニストであるKN-93とH89は,Ser19とSer40でのACh誘導リン酸化を阻害し,SMIの添加は阻害効果を増強しなかった。まとめると,著者らの結果は,SMIがそのSer19とSer40部位でTH活性を遮断することによりCA分泌を阻害することを示した。Copyright 2020 Xiting Zhang et al. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *カテコールアミン ,  *受容体 ,  チロシンヒドロキシラーゼ ,  *副腎 ,  リン酸化 ,  タンパク質キナーゼ ,  心血管疾患 ,  カルモジュリン ,  *イオンチャンネル ,  *ニコチン受容体 ,  タンパク質キナーゼA ,  脂肪酸
準シソーラス用語： αサブユニット ,  アンタゴニスト ,  *副腎髄質細胞 , 【Automatic Indexing@JST】

分類 (5件)： 生薬の薬理の基礎研究  (GX06020J) ,  中枢神経系作用薬一般  (GW03010A) ,  細胞膜の受容体  (EF04030C) ,  細胞生理一般  (EF02010S) ,  内分泌系の生理と解剖学  (EJ08000J)

物質索引 (1件)： アセチルコリン

引用文献 (29件)：

• L. Jian-Sheng, W. Hai-Feng, L. Su-Yun et al., "Shenmai injection for chronic pulmonary heart disease: a systematic review and meta-analysis," Journal of Alternative and Complementary Medicine, vol. 17, no. 7, pp. 579-587, 2011.
• Y. Shinohara, Y. Toyohira, S. Ueno, M. Liu, M. Tsutsui, N. Yanagihara, "Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells," Biochemical Pharmacology, vol. 74, no. 11, pp. 1608-1618, 2007.
• X. Li, Y. Toyohira, T. Horisita et al., "Ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppressing nicotinic acetylcholine receptor-ion channels in cultured bovine adrenal medullary cells," Naunyn-Schmiedeberg’s Archives of Pharmacology, vol. 388, no. 12, pp. 1259-1269, 2015.
• M. Taeko, T. Yumiko, U. Susumu et al., "Simvastatin inhibits catecholamine secretion and synthesis induced by acetylcholine via blocking Na⁺ and Ca²⁺ influx in bovine adrenal medullary cells," Journal of Pharmacology & Experimental Therapeutics, vol. 327, no. 1, pp. 130-136, 2008.
• M. Haoping, W. Hong, M. Shangwei et al., "Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion," Toxicology & Applied Pharmacology, vol. 274, no. 1, pp. 180-189, 2014.

タイトルに関連する用語 (12件)： 培養 ,  ウシ ,  副腎髄質細胞 ,  ニコチン性アセチルコリン受容体 ,  イオンチャンネル ,  調節 ,  アセチルコリン ,  誘発 ,  カテコールアミン ,  分泌 ,  注射 ,  抑制効果