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J-GLOBAL ID：202102280790817995   整理番号：21A0829194

腫瘍発現を反映している血清および尿細胞外小胞における胆管癌患者特異的RNAプロファイルの患者:疾患診断のための新規液体生検バイオマーカー【JST・京大機械翻訳】

Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis

著者 (37件)： Lapitz Ainhoa (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Arbelaiz Ander (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  O’Rourke Colm J. (Biotech Research & Innovation Centre (BRIC), Department of Health and Medical Sciences, 2200, Copenhagen, Denmark) ,  Lavin Jose L. (CIC bioGUNE, Genome Analysis Platform, 48160, Derio, Spain) ,  Casta Adelaida La (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Ibarra Cesar (Hospital of Cruces, 48903, Bilbao, Spain) ,  Jimeno Juan P. (“Complejo Hospitalario de Navarra”, 31008, Pamplona, Spain) ,  Santos-Laso Alvaro (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Izquierdo-Sanchez Laura (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Izquierdo-Sanchez Laura (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Krawczyk Marcin (Department of Medicine II, Saarland University Medical Centre, Saarland University, 66421, Homburg, Germany) ,  Krawczyk Marcin (Laboratory of Metabolic Liver Diseases, Centre for Preclinical Research, Department of General, Transplant and Liver Surgery, 02-091, Warsaw, Poland) ,  Perugorria Maria J. (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Perugorria Maria J. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Jimenez-Aguero Raul (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Sanchez-Campos Alberto (Hospital of Cruces, 48903, Bilbao, Spain) ,  Riano Ioana (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Gonzalez Esperanza (Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Exosomes Laboratory, 48160, Derio, Spain) ,  Lammert Frank (Department of Medicine II, Saarland University Medical Centre, Saarland University, 66421, Homburg, Germany) ,  Marzioni Marco (“Universita Politecnica delle Marche”, Department of Gastroenterology, 60121, Ancona, Italy) ,  Macias Rocio I.R. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Macias Rocio I.R. (Experimental Hepatology and Drug Targeting (HEVEFARM), Biomedical Research Institute of Salamanca (IBSAL), 37007, Salamanca, Spain) ,  Marin Jose J. G. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Marin Jose J. G. (Experimental Hepatology and Drug Targeting (HEVEFARM), Biomedical Research Institute of Salamanca (IBSAL), 37007, Salamanca, Spain) ,  Karlsen Tom H. (Norwegian PSC Research Center, Division of Cancer Medicine, Surgery and Transplantation, Oslo University Hospital, 0372, Oslo, Spain) ,  Bujanda Luis (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Bujanda Luis (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Falcon-Perez Juan M. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Falcon-Perez Juan M. (Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Exosomes Laboratory, 48160, Derio, Spain) ,  Falcon-Perez Juan M. (IKERBASQUE, Basque Foundation for Science, 48013, Bilbao, Spain) ,  Andersen Jesper B. (Biotech Research & Innovation Centre (BRIC), Department of Health and Medical Sciences, 2200, Copenhagen, Denmark) ,  Aransay Ana M. (CIC bioGUNE, Genome Analysis Platform, 48160, Derio, Spain) ,  Aransay Ana M. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Rodrigues Pedro M. (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Banales Jesus M. (Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), 20014, San Sebastian, Spain) ,  Banales Jesus M. (Carlos III National Institute of Health, Center for the Study of Liver and Gastrointestinal Diseases (CIBERehd), 28220, Madrid, Spain) ,  Banales Jesus M. (IKERBASQUE, Basque Foundation for Science, 48013, Bilbao, Spain)
資料名： Cells (Web)  (Cells (Web))
巻： 9  号： 3  ページ： 721  発行年： 2020年 
JST資料番号： U7155A  ISSN： 2073-4409  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： スイス (CHE)  言語： 英語 (EN)

抄録/ポイント： 胆管癌(CCA)は,予後不良の不均一な胆道癌群から成る。ほとんどのCCAの病因は不明であるが,原発性硬化性胆管炎(PSC)は危険因子である。CCAの非侵襲的診断は困難であり,正確なバイオマーカーは不足している。CCA,PSC,潰瘍性大腸炎(UC)および健常人の患者の血清および尿細胞外小胞(EVs)のトランスクリプトームプロファイルを特性化することを目的とした。血清および尿EVを連続超遠心により分離し,ナノ粒子追跡分析,透過型電子顕微鏡および免疫ブロッティングにより特性化した。EVsトランスクリプトームは,Illumina遺伝子発現配列[メセンジャーRNA(mRNA)と非コードRNA(ncRNA)]によって決定された。差次的RNAプロフィールは,対照群(疾患および健康)と比較してCCA患者由来の血清および尿EVsにおいて見出され,高い診断能力を示した。CCA患者からの血清または尿EVsのmRNAプロファイルを,患者の2つのコホート,in vitroでのCCA細胞,およびCCA細胞由来EVsから,それぞれ105および39の共通に変化した転写物を同定した。遺伝子オントロジー分析は,最も一般的なmRNAが発癌性段階に関与することを示した。全体として,CCA患者は腫瘍を鏡視するEVsにおいて特異的RNAプロファイルを示し,新しい有望な液体生検バイオマーカーを構成する。Copyright 2021 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *RNA【核酸】 ,  mRNA ,  *血清 ,  超遠心 ,  母集団 ,  遺伝子発現 ,  in vitro実験 ,  *バイオマーカー ,  病因 ,  危険因子 ,  *腫瘤形成 ,  ナノ粒子 ,  トランスクリプトーム
準シソーラス用語： *胆管癌 ,  予後不良 , 【Automatic Indexing@JST】

著者キーワード (5件)： バイオマーカー ,  胆管癌 ,  細胞外小胞 ,  液体生検 ,  トランスクリプトミクス

分類 (3件)： 消化器の疾患  (GH03000W) ,  消化器の診断  (GH02000P) ,  消化器の腫よう  (GH04000D)

引用文献 (89件)：

• Banales, J.M.; Cardinale, V.; Carpino, G.; Marzioni, M.; Andersen, J.B.; Invernizzi, P.; Lind, G.E.; Folseraas, T.; Forbes, S.J.; Fouassier, L.; et al. Expert consensus document: Cholangiocarcinoma: Current knowledge and future perspectives consensus statement from the European Network for the Study of Cholangiocarcinoma (ENS-CCA). Nat. Rev. Gastroenterol. Hepatol. 2016, 13, 261-280.
• Rizvi, S.; Khan, S.A.; Hallemeier, C.L.; Kelley, R.K.; Gores, G.J. Cholangiocarcinoma-Evolving concepts and therapeutic strategies. Nat. Rev. Clin. Oncol. 2018, 15, 95-111.
• Bertuccio, P.; Malvezzi, M.; Carioli, G.; Hashim, D.; Boffetta, P.; El-Serag, H.B.; La Vecchia, C.; Negri, E. Global trends in mortality from intrahepatic and extrahepatic cholangiocarcinoma. J. Hepatol. 2019, 71, 104-114.
• Karlsen, T.H.; Folseraas, T.; Thorburn, D.; Vesterhus, M. Primary sclerosing cholangitis-A comprehensive review. J. Hepatol. 2017, 67, 1298-1323.
• Razumilava, N.; Gores, G.J. Surveillance for cholangiocarcinoma in patients with primary sclerosing cholangitis: Effective and justified? Clin. Liver Dis. 2016, 8, 43-47.

タイトルに関連する用語 (13件)： 腫瘍発現 ,  血清 ,  尿 ,  細胞外小胞 ,  胆管 ,  癌患者 ,  RNA ,  プロファイル ,  患者 ,  疾患 ,  診断 ,  液体生検 ,  バイオマーカー