文献

J-GLOBAL ID：202202210137401966   整理番号：22A0003102

容易に入手可能な試薬を用いたSARS-CoV-2試験のための磁性ビーズベース核酸抽出の最適化【JST・京大機械翻訳】

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

著者 (34件)： Haile Simon (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Nikiforuk Aidan M. (School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada) ,  Pandoh Pawan K. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Twa David D.W. (BC Cancer Research Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Smailus Duane E. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Nguyen Jason (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Pleasance Stephen (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Wong Angus (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Zhao Yongjun (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Eisler Diane (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Moksa Michelle (Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada) ,  Cao Qi (Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada) ,  Wong Marcus (Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada) ,  Su Edmund (Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada) ,  Krzywinski Martin (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Nelson Jessica (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Mungall Andrew J. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Tsang Frankie (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Prentice Leah M. (Provincial Laboratory Medicine Services, Provincial Health Services Authority, Vancouver, British Columbia, Canada) ,  Jassem Agatha (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Jassem Agatha (Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada) ,  Manges Amee R. (School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada) ,  Manges Amee R. (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Jones Steven J.M. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Jones Steven J.M. (Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada) ,  Coope Robin J. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Prystajecky Natalie (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Prystajecky Natalie (Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada) ,  Marra Marco A. (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Marra Marco A. (Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada) ,  Krajden Mel (British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada) ,  Krajden Mel (Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada) ,  Hirst Martin (Canada’s Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada) ,  Hirst Martin (Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada)
資料名： Journal of Virological Methods  (Journal of Virological Methods)
巻： 299  ページ： Null  発行年： 2022年 
JST資料番号： E0807B  ISSN： 0166-0934  CODEN： JVMEDH  資料種別： 逐次刊行物 (A)
記事区分： 短報  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： COVID-19パンデミックは,診断試験における一般的試薬と柔軟なシステムの必要性を強調した。オープン液体ハンドラー上の96穴プレートを用いた磁気ビーズベースの核酸抽出プロトコルは,このニーズに応えるのに容易である。ここでは,感度を維持しながら,クロスウェル汚染を最小化するために,そのようなアプローチを厳密に最適化した。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *最適化

著者キーワード (8件)： SARS-CoV-2 ,  COVID-19 ,  核酸抽出 ,  RNA ,  交差井戸 ,  汚染 ,  Hamilton NIMBUS ,  磁気ビーズ

分類 (1件)： 核酸一般  (EB04010U)

タイトルに関連する用語 (7件)： 入手 ,  試薬 ,  SARS-CoV-2 ,  試験 ,  磁性ビーズ ,  核酸抽出 ,  最適化