文献

J-GLOBAL ID：202202240432564084   整理番号：22A1074290

スパースデコンボリューションが生細胞の超高分解能蛍光顕微鏡法を改善する

Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy

著者 (35件)： ZHAO Weisong (Advanced Microscopy and Instrumentation Res. Center, School of Instrumentation Sci. and Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  QIU Guohua (Advanced Microscopy and Instrumentation Res. Center, School of Instrumentation Sci. and Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  HAN Zhenqian (Advanced Microscopy and Instrumentation Res. Center, School of Instrumentation Sci. and Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  LIU Jian (Advanced Microscopy and Instrumentation Res. Center, School of Instrumentation Sci. and Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  LI Haoyu (Advanced Microscopy and Instrumentation Res. Center, School of Instrumentation Sci. and Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  ZHAO Shiqun (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  LI Liuju (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  XING Shijia (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  ZHANG Yulin (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  WU Runlong (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  MO Yanquan (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  LIU Yanmei (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  CHEN Liangyi (State Key Lab. of Membrane Biology, Beijing Key Lab. of Cardiometabolic Molecular Medicine, Inst. of Molecular Medicine, National Biomedical Imaging Center, School of Future Technol., Peking Univ., Beijing, CHN) ,  HUANG Xiaoshuai (Biomedical Engineering Dep., Peking Univ., Beijing, CHN) ,  SHANG Yingxu (CAS Key Lab. of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technol., Beijing, CHN) ,  DING Baoquan (CAS Key Lab. of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technol., Beijing, CHN) ,  SUN De-en (Coll. of Chemistry and Molecular Engineering, Peking Univ., Beijing, CHN) ,  CHEN Xing (Coll. of Chemistry and Molecular Engineering, Peking Univ., Beijing, CHN) ,  SHAN Chunyan (Coll. of Life Sci., Peking Univ., Beijing, CHN) ,  GU Lusheng (National Lab. of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Inst. of Biophysics, Chinese Acad. of Sci., Beijing, CHN) ,  ZHANG Shuwen (National Lab. of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Inst. of Biophysics, Chinese Acad. of Sci., Beijing, CHN) ,  JI Wei (National Lab. of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Inst. of Biophysics, Chinese Acad. of Sci., Beijing, CHN) ,  CHEN Riwang (School of Software and Microelectronics, Peking Univ., Beijing, CHN) ,  WANG Jianyong (School of Software and Microelectronics, Peking Univ., Beijing, CHN) ,  XIAO Jian (Hubei Key Lab. of Cell Homeostasis, Coll. of Life Sci., Wuhan Univ., Wuhan, CHN) ,  SONG Bao-Liang (Hubei Key Lab. of Cell Homeostasis, Coll. of Life Sci., Wuhan Univ., Wuhan, CHN) ,  LIU Yanmei (Inst. for Brain Res. and Rehabilitation (IBRR), Guangdong Key Lab. of Mental Health and Cognitive Sci., South China Normal Univ., Guangzhou, CHN) ,  MAO Heng (School of Mathematical Sci., Peking Univ., Beijing, CHN) ,  TAN Jiubin (Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Inst. of Technol., Harbin, CHN) ,  TAN Jiubin (Key Lab. of Ultra-Precision Intelligent Instrumentation of Ministry of Ind. and Information Technol., Harbin Inst. of Technol., Harbin, CHN) ,  LI Haoyu (Key Lab. of Ultra-Precision Intelligent Instrumentation of Ministry of Ind. and Information Technol., Harbin Inst. of Technol., Harbin, CHN) ,  LIU Jian (Key Lab. of Micro-Systems and Micro-Structures Manufacturing of Ministry of Education, Harbin Inst. of Technol., Harbin, CHN) ,  LI Haoyu (Lab. for Space Environment and Physical Sci., Harbin Inst. of Technol., Harbin, CHN) ,  CHEN Liangyi (PKU-IDG/McGovern Inst. for Brain Res., Beijing, CHN) ,  CHEN Liangyi (Beijing Acad. of Artificial Intelligence, Beijing, CHN)
資料名： Nature Biotechnology  (Nature Biotechnology)
巻： 40  号： 4  ページ： 606-617  発行年： 2022年04月 
JST資料番号： H0870A  ISSN： 1087-0156  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： アメリカ合衆国 (USA)  言語： 英語 (EN)

抄録/ポイント： 生細胞の超高分解能(SR)顕微鏡法では,収集可能な最大光子束が空間分解能の主要な決定因子となっている。所与の光子束の有効分解能をさらに高めるために,我々は,生物学的構造体の希薄性と連続性に関する経験的知識を利用して,SR顕微鏡の分解能をほぼ2倍に高めるデコンボリューションアルゴリズムを開発した。Sparse-SIM(sparse structured illumination microscopy)と称するこの方法は,564Hzまでのフレーム速度で約60nmの分解能を達成し,生細胞中の小さな小胞融合細孔,ヌクレオポリンが形成する環状の核膜孔,ミトコンドリアの内膜と外膜の相対運動など,複雑な構造を解像することができる。また,スパースデコンボリューションを用いると,信号対雑音比が低くてもスピニングディスク共焦点法に基づくSIMの三次元分解能を上げることができ,分解能約90nmの4色三次元生細胞SRイメージングが可能になった。以上により,スパースデコンボリューションは生細胞蛍光顕微鏡法の時空間分解能向上に有用と考えられる。Copyright Nature Japan KK 2022

シソーラス用語： SN比 ,  アルゴリズム ,  光子 ,  *細胞 ,  ミトコンドリア ,  *顕微鏡観察 ,  *デコンボリューション ,  画像処理 ,  *光学顕微鏡法 ,  三次元 ,  構造体
準シソーラス用語： フレームレート ,  *蛍光顕微鏡法 ,  *生細胞 ,  外膜 ,  共焦点 ,  *超高解像度 , 【Automatic Indexing@JST】

分類 (1件)： 生体の顕微鏡観察法  (EA03050C)

タイトルに関連する用語 (3件)： 生細胞 ,  高分解能 ,  蛍光顕微鏡法