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J-GLOBAL ID：202202244016458223   整理番号：22A0747824

ラット肝細胞におけるオレイン酸誘導酸化損傷および脂肪症に及ぼすイソオリエンチンの保護作用【JST・京大機械翻訳】

Protective Effect of Isoorientin on Oleic Acid-Induced Oxidative Damage and Steatosis in Rat Liver Cells

著者 (45件)： Luo Tongwang (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Luo Tongwang (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Luo Tongwang (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Luo Tongwang (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Luo Tongwang (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Jiang Sheng (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Jiang Sheng (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Jiang Sheng (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Jiang Sheng (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Jiang Sheng (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Zhou Bin (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Zhou Bin (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Zhou Bin (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Zhou Bin (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Zhou Bin (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Song Quanjiang (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Song Quanjiang (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Song Quanjiang (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Song Quanjiang (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Song Quanjiang (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Du Jing (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Du Jing (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Du Jing (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Du Jing (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Du Jing (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Liu Ping (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Liu Ping (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Liu Ping (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Liu Ping (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Liu Ping (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Wang Xiaodu (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Wang Xiaodu (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Wang Xiaodu (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Wang Xiaodu (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Wang Xiaodu (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Song Houhui (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Song Houhui (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Song Houhui (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Song Houhui (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Song Houhui (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China) ,  Shao Chunyan (1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China) ,  Shao Chunyan (2Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China) ,  Shao Chunyan (3Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China) ,  Shao Chunyan (4China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China) ,  Shao Chunyan (5College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China)
資料名： Frontiers in Pharmacology (Web)  (Frontiers in Pharmacology (Web))
巻： 13  ページ： 818159  発行年： 2022年 
JST資料番号： U7091A  ISSN： 1663-9812  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： スイス (CHE)  言語： 英語 (EN)

抄録/ポイント： ヒト健康への非アルコール性脂肪肝疾患の害は増加しており,それは病気の緊急予防と治療を必要とする。イソオリエンチンは,抗炎症および抗酸化作用を有する漢方薬の有効成分である。しかし,非アルコール性脂肪肝疾患におけるイソオリエンチンの効果はまだ不明である。本研究では,in vivoおよびin vitro実験,病理観察,フローサイトメトリー,免疫蛍光およびウェスタンブロット分析により,脂肪変性におけるイソオリエンチンの役割を調べ,その分子機構を明らかにした。結果は,オレイン酸処理がラット肝細胞におけるROSと脂質滴の含有量を有意に増加させ,γH2AX,HO-1,PPARγ,SREBP-1c,FASの発現を促進することを示した。イソオリエンチンとオレイン酸で共処理した細胞のROS含有量は,オレイン酸群と比較して有意に減少し,γH2AX,HO-1,PPARγ,SREBP-1c,FAS,およびNF-κB p65の核転座も有意に阻害された。著者らのデータは,オレイン酸がin vitroおよびin vivoの両方で肝細胞において酸化的損傷および脂肪変性を誘導し,PPARγ/NF-κB p65シグナル経路を活性化することを示した。さらに,イソオリエンチンはPPARγ/NF-κB p65シグナル経路の調節によりオレイン酸誘導酸化損傷と脂肪変性を有意に減少させることができる。Copyright 2022 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 消炎作用 ,  脂肪肝 ,  ヒストン ,  ヒト ,  *ラット ,  機構 ,  フローサイトメトリー ,  転座 ,  病理 ,  漢方製剤 ,  ウェスタンブロット法 ,  *肝細胞 ,  PPARγ ,  不飽和脂肪酸 ,  オレフィン化合物
準シソーラス用語： 分子機構 ,  p65 ,  γH2AX , 【Automatic Indexing@JST】

著者キーワード (6件)： イソオリエンチン ,  オレイン酸 ,  脂肪肝 ,  酸化損傷 ,  PPARγ ,  NF-κB p65

分類 (1件)： 消化器の基礎医学  (GH01020E)

物質索引 (1件)： オレイン酸

引用文献 (40件)：

• AlbaghdadiM. S., IkegamiR., KassabM. B., GardeckiJ. A., KunioM., ChowdhuryM. M., et al (2021). Near-Infrared Autofluorescence in Atherosclerosis Associates with Ceroid and Is Generated by Oxidized Lipid-Induced Oxidative Stress. Arterio. Throm. Vas. Bio. 41, e385-e398. doi: 10.1161/ATVBAHA.120.315612
• ChenC., MaharR., MerrittM. E., DenlingerD. L., HahnD. A. (2021). ROS and Hypoxia Signaling Regulate Periodic Metabolic Arousal during Insect Dormancy to Coordinate Glucose, Amino Acid, and Lipid Metabolism. Proc. Natl. Acad. Sci. U. S. A. 118, e2017603118. doi: 10.1073/pnas.2017603118
• ChenS., LuoT., YuQ., DongW., ZhangH., ZouH. (2020). Isoorientin Plays an Important Role in Alleviating Cadmium-Induced DNA Damage and G0/G1 Cell Cycle Arrest. Ecotoxicol Environ. Saf. 187, 109851. doi: 10.1016/j.ecoenv.2019.109851
• CollinsP. L., PurmanC., PorterS. I., NgangaV., SainiA., HayerK. E., et al (2020). DNA Double-Strand Breaks Induce H2Ax Phosphorylation Domains in a Contact-dependent Manner. Nat. Commun. 11, 3158. doi: 10.1038/s41467-020-16926-x
• FanX., WeiW., HuangJ., LiuX., CiX. (2020). Isoorientin Attenuates Cisplatin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis via Activating the SIRT1/SIRT6/Nrf-2 Pathway. Front Pharmacol. 11, 264. doi: 10.3389/fphar.2020.00264

タイトルに関連する用語 (7件)： ラット ,  肝細胞 ,  オレイン酸 ,  損傷 ,  脂肪症 ,  イソオリエンチン ,  保護作用