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J-GLOBAL ID：202202252361457894   整理番号：22A0000096

T7ヌクレアーゼ支援サイクリング酵素増幅に基づく腫瘍細胞におけるサークRNA定量化のための蛍光戦略【JST・京大機械翻訳】

A fluorescence strategy for circRNA quantification in tumor cells based on T7 nuclease-assisted cycling enzymatic amplification

著者 (29件)： Qu Hongke (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Qu Hongke (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Chen Mingjian (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Ge Junshang (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Zhang Xiangyan (Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan, China) ,  He Shuyi (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Xiong Fang (Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Yan Qijia (Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Zhang Shanshan (Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Gong Zhaojian (Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Guo Can (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Guo Can (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Wang Fuyan (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Wang Fuyan (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Zeng Zhaoyang (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Zeng Zhaoyang (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Zeng Zhaoyang (Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Li Xiaoling (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Li Xiaoling (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Li Xiaoling (Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Li Guiyuan (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Li Guiyuan (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Li Guiyuan (Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Xiong Wei (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Xiong Wei (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Xiong Wei (Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China) ,  Wu Xu (NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China) ,  Wu Xu (Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, China) ,  Wu Xu (Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China)
資料名： Analytica Chimica Acta  (Analytica Chimica Acta)
巻： 1189  ページ： Null  発行年： 2022年 
JST資料番号： A0394A  ISSN： 0003-2670  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 環状リボ核酸(CircRNA)は,腫瘍や代謝性疾患のような多くの生物学的過程において調節的役割を果たす。循環RNAが線形RNAより安定かつ保存的であるという事実により,サーコRNAは早期臨床診断および生物医学研究における潜在的バイオマーカーになった。したがって,循環RNA発現レベルの定量化は,それらの機能および疾患診断および治療への応用を理解するために重要である。それにもかかわらず,循環RNAの低い存在量のため,それは細胞中のサーコRNAの分析にとってまだ課題である。ここでは,T7エキソヌクレアーゼ支援サイクリング酵素増幅に基づくサーコRNAの高感度検出法を提案した。ヘアピン分子ビーコンとT7エキソヌクレアーゼにより蛍光センサを構築した。サイクリング酵素増幅プロセスにより,このセンサは1pMの検出限界を達成し,モデルとしてcircBART2.2を用いて0~100pM(R2=0.9891)の範囲で良好な線形相関を示した。さらに,著者らは,細胞溶解物中の循環BART2.2の定量において,提案した方法を適用した。結果は,本法が,バイオマーカーとしてサーコRNAを用いたEpstein-Barrウイルス(EBV)感染関連疾患の早期診断に有望な応用を有することを示した。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： *蛍光 ,  *酵素 ,  *RNA【核酸】 ,  エキソヌクレアーゼ ,  *腫瘍 ,  *腫瘍細胞 ,  *ヌクレアーゼ ,  検出法 ,  代謝性疾患 ,  検出限界 ,  早期診断 ,  バイオマーカー
準シソーラス用語： 分子ビーコン ,  臨床診断 ,  細胞溶解 , 【Automatic Indexing@JST】

著者キーワード (5件)： 蛍光センサ ,  分子ビーコン ,  酵素支援サイクリング増幅 ,  CircRNA ,  CircBART2.2

分類 (2件)： 核酸一般  (EB04010U) ,  腫ようの診断  (GE03020J)

タイトルに関連する用語 (11件)： ヌクレアーゼ ,  支援 ,  サイクリング ,  酵素 ,  増幅 ,  腫瘍細胞 ,  サーク ,  RNA ,  定量化 ,  蛍光 ,  戦略