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J-GLOBAL ID：202202263892663538   整理番号：22A0624042

転写を調節するリジン2-ヒドロキシイソブチリルトランスフェラーゼとしてのTmcA機能【JST・京大機械翻訳】

TmcA functions as a lysine 2-hydroxyisobutyryltransferase to regulate transcription

著者 (13件)： Dong Hanyang (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Dong Hanyang (The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, China) ,  Zhao Yujie (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Bi Changfen (Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Sciences, Tianjin, China) ,  Han Yue (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Zhang Jianji (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Bai Xue (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Zhai Guijin (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Zhang Hui (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Tian Shanshan (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...) ,  Hu Deqing (Tianjin Key Laboratory of Medical Epigenetics, Department of Cell Biology, Tianjin Medical University, Tianjin, China) ,  Xu Liyan (The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, China) ,  Zhang Kai (The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Mol...)
資料名： Nature Chemical Biology  (Nature Chemical Biology)
巻： 18  号： 2  ページ： 142-151  発行年： 2022年 
JST資料番号： W2213A  ISSN： 1552-4450  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： ドイツ (DEU)  言語： 英語 (EN)

抄録/ポイント： 蛋白質リジン2-ヒドロキシイソブチリル化(Khib)は,最近,細胞過程の調節に重要な役割を果たすことが示されている。しかしながら,原核生物におけるKhibの機構と機能的結果は不明のままである。ここでは,RNAアセチルトランスフェラーゼであるTmcAが転写の調節においてリジン2-ヒドロキシイソブチリルトランスフェラーゼとして機能することを報告する。TmcAはin vitroと細胞内の両方でKhibを効果的に触媒し,R502はTmcAのKhib触媒活性の鍵となる部位であることを示した。定量的プロテオミクスを用いて,大腸菌のKhibに対するTmcAにより標的化された467の内因性候補を同定した。興味深いことに,TmcAはK121でのKhibの触媒作用によりヌクレオイド関連蛋白質であるH-NSのDNA結合活性を特異的に調節できることを証明した。さらに,このTmcA標的化Khibは酸耐性遺伝子の転写を調節し,酸ストレス下で大腸菌の生存を促進する。この研究は,細菌酸耐性に対するTmcA触媒Khibにより仲介される転写調節を明らかにした。RNAアセチルトランスフェラーゼTmcAは原核生物の酸ストレスに応答して細菌転写を調節するリジン2-ヒドロキシイソブチリルトランスフェラーゼとして機能する。Copyright The Author(s), under exclusive licence to Springer Nature America, Inc. 2021 Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 細菌 ,  大腸菌 ,  触媒活性 ,  RNA【核酸】 ,  遺伝子 ,  耐酸性 ,  遺伝的調節 ,  *原核生物 ,  アセチルトランスフェラーゼ ,  ターゲティング ,  in vitro実験
準シソーラス用語： 触媒作用 ,  酸ストレス ,  内因性 ,  DNA結合 ,  転写調節 , 【Automatic Indexing@JST】

分類 (3件)： 遺伝子発現  (EC02040Q) ,  酵素の応用関連  (EB09100B) ,  酵素一般  (EB09010D)

タイトルに関連する用語 (3件)： 転写 ,  調節 ,  リジン