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J-GLOBAL ID：202202267509327014   整理番号：22A1090653

糖工学脂肪由来間葉細胞による髄核修復の増強【JST・京大機械翻訳】

Enhancement of nucleus pulposus repair by glycoengineered adipose-derived mesenchymal cells

著者 (51件)： Ying Liwei (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Ying Liwei (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Ying Liwei (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Liang Chengzhen (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Liang Chengzhen (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Liang Chengzhen (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yujie (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yujie (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yujie (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Wang Jingkai (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Wang Jingkai (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Wang Jingkai (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Wang Chenggui (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Wang Chenggui (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Wang Chenggui (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Xia Kaishun (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Xia Kaishun (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Xia Kaishun (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Shi Kesi (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Shi Kesi (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Shi Kesi (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Yu Chao (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Yu Chao (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Yu Chao (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Yang Biao (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Yang Biao (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Yang Biao (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Xu Haibin (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Xu Haibin (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Xu Haibin (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yuang (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yuang (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Zhang Yuang (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Shu Jiawei (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Shu Jiawei (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Shu Jiawei (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Huang Xianpeng (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Huang Xianpeng (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Huang Xianpeng (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Xing Hongyuan (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Xing Hongyuan (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Xing Hongyuan (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Li Fangcai (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Li Fangcai (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Li Fangcai (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Zhou Xiaopeng (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Zhou Xiaopeng (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Zhou Xiaopeng (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China) ,  Chen Qixin (Department of Orthopedics Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, PR China) ,  Chen Qixin (Orthopedics Research Institute of Zhejiang University, Hangzhou City, Zhejiang Province, PR China) ,  Chen Qixin (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou City, Zhejiang Province, PR China)
資料名： Biomaterials  (Biomaterials)
巻： 283  ページ： Null  発行年： 2022年 
JST資料番号： C0964B  ISSN： 0142-9612  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： 脂肪由来間葉系幹細胞(ADSCs)は,i.i.i.炎症およびiiを調節する生物活性因子の分泌。髄核(NP)様細胞に分化する可能性は,宿主組織に統合できる。しかしながら,NP様細胞に対するADSCの分化能は制限され,細胞分化を調節するための代替アプローチの必要性を強調する。細胞機能が細胞外マトリックス(ECM)と細胞間の相互作用により影響されることを考えると,細胞表面修飾がNP様細胞へのADSC接着と分化を促進すると仮定した。本研究では,ADSCの細胞表面を代謝糖工学を介して非天然シアル酸で機能化した。続いて,3つの主なECM(ラミニン,コラーゲンおよびフィブロネクチン)に対する修飾細胞の接着能を比較した。接着アッセイは,グリコエンジニアリングADSCがラミニンおよびフィブロネクチンと比較し,コラーゲンに対し最も高い親和性を有することを明らかにした。さらに,コラーゲン被覆プレートによる培養は,NP様細胞へのグリコエンジニアリングADSCの分化を増強した。代謝糖工学はADSC生存率を延長した。糖工学ADSCは,髄核の含水量とECM体積と同様に,椎間板の高さと弾性を増加させた。結論として,細胞表面の代謝糖工学は,細胞生物学的機能の調節およびNP組織修復の促進において重要な役割を持つ。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 接着 ,  相互作用 ,  弾性 ,  被覆 ,  炎症 ,  *コラーゲン ,  分化 ,  生物活性 ,  生存率 ,  ラミニン ,  フィブロネクチン ,  細胞分化 ,  細胞外マトリックス
準シソーラス用語： 細胞表面 ,  *髄核 , 【Automatic Indexing@JST】

著者キーワード (5件)： 代謝糖鎖工学 ,  N-プロピオニルマンノサミン ,  接着 ,  分化 ,  インテグリン

分類 (2件)： 医用素材  (GA05040H) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (7件)： 糖 ,  工学 ,  脂肪 ,  間葉細胞 ,  髄核 ,  修復 ,  増強