文献

J-GLOBAL ID：202202277077131622   整理番号：22A0176781

変形性関節症滑液-エキソソーム研究におけるエキソソームmRNA,lncRNAおよびcircRNAシグネチャの同定【JST・京大機械翻訳】

Identification of exosomal mRNA, lncRNA and circRNA signatures in an osteoarthritis synovial fluid-exosomal study

著者 (40件)： Wu Xiaomin (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Wu Xiaomin (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Wu Xiaomin (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Wu Xiaomin (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Bian Bin (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Bian Bin (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Bian Bin (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Bian Bin (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Lin Zhuofeng (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Lin Zhuofeng (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Lin Zhuofeng (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Lin Zhuofeng (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Wu Chenxi (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Wu Chenxi (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Wu Chenxi (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Wu Chenxi (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Sun Yuxin (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Sun Yuxin (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Sun Yuxin (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Sun Yuxin (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Pan Yu (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Pan Yu (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Pan Yu (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Pan Yu (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Dai Yong (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Dai Yong (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Dai Yong (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Dai Yong (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Lui Tun Hing (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Lui Tun Hing (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Lui Tun Hing (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Lui Tun Hing (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Zhuang Tengfei (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Zhuang Tengfei (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Zhuang Tengfei (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Zhuang Tengfei (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China) ,  Pan Xiaohua (Department of Orthopaedics, The Second Affiliated Hospital of Shenzhen University, The Second School of Clinical Medicine, Southern Medical University, The Clinical Medical College of Guangdong Medical University, People’s Hospital of Shenzhen Baoan District, China) ,  Pan Xiaohua (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen, China) ,  Pan Xiaohua (Department of Orthopaedic and Traumatology, North District hospital, Sheung Shui, Hong Kong) ,  Pan Xiaohua (Shenzhen Yspring Technology Co.., Ltd, Shenzhen, PR China)
資料名： Experimental Cell Research  (Experimental Cell Research)
巻： 410  号： 1  ページ： Null  発行年： 2022年 
JST資料番号： B0313A  ISSN： 0014-4827  CODEN： ECREAL  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 変形性関節症(OA)は,関節マトリックスの破壊および関節軟骨の減少により病理学的に特徴付けられる一般的な変性疾患であり,関節変形および運動機能障害を生じる。しかし,この病理学を支配する分子機構はこれまで解明されていない。本研究では,OAおよび対照患者の滑膜エキソソームから抽出したlncRNA,circRNAおよびmRNAの発現レベルを測定した。循環RNA/lncRNA-miRNA-mRNA相互作用のネットワークを,OA病因を探索するためにMiRandaとTargetScanソフトウェアを用いて確立した。OA及び対照群のエキソソームlncRNA,circRNA及びmRNA発現プロファイルをLCヒト競合内因性RNA(ceRNA)マイクロアレイを用いて解析した。差次的発現遺伝子を分析し,生物情報学分析によってOAの病因におけるそれらの潜在的役割を決定した。52のmRNA,196のlncRNAと98のcircRNAが,変形性関節症滑膜と対照群の間で滑膜エキソソームで差別的に発現した。lncRNAとcircRNAの最終ceRNAネットワークは,OAの病理学的メカニズムに関連したncRNAとmRNAの間の複雑な相互作用を示した。ceRNAネットワークの交差分析は,PI3K/Aktとオートファジー経路に富む22のmiRNA,45のlncRNA,および34のcircRNAが7つのmRNAと相関し,OAの病理学的機序において重要な役割を果たすかもしれないことを示した。本研究では,mRNA/lncRNA/circRNA発現を分析し,滑膜エキソソームにおけるOAの病因をプロファイルするためにlncRNAとcircRNAのceRNAネットワークを示した。本研究の結果はOAの病因の解明を助け,OAの診断と治療のためのより効果的な標的を同定するさらなる研究の試みに重要な参照を提供するかもしれない。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 相互作用 ,  ネットワーク ,  *RNA【核酸】 ,  *mRNA ,  遺伝子 ,  ヒト ,  ソフトウェア ,  *変形性関節症 ,  病因 ,  オートファジー
準シソーラス用語： マイクロRNA ,  分子機構 ,  *エキソソーム ,  mRNA発現 ,  *環状RNA , 【Automatic Indexing@JST】

著者キーワード (5件)： 変形性関節症 ,  滑膜エキソソーム ,  競合する内因性RNA ,  PI3K/AKT経路 ,  オートファジー経路

分類 (2件)： 細胞構成体の機能  (EF03060C) ,  細胞生理一般  (EF02010S)

タイトルに関連する用語 (9件)： 変形性関節症 ,  滑液 ,  エキソソーム ,  研究 ,  mRNA ,  lncRNA ,  circRNA ,  シグネチャ ,  同定