文献

J-GLOBAL ID：202202282036747752   整理番号：22A0737860

免疫調節性細胞外小胞負荷注入可能温度感受性ヒドロゲル後の脊髄損傷後の運動機能回復の増強【JST・京大機械翻訳】

Enhancement of motor functional recovery using immunomodulatory extracellular vesicles-loaded injectable thermosensitive hydrogel post spinal cord injury

著者 (40件)： Zhang Zengjie (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Zhang Zengjie (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Zhang Zengjie (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Zhang Xiaolei (Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, West Xueyuan Road 109#, Wenzhou 325027, Zhejiang Province, PR China) ,  Zhang Xiaolei (Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, Zhejiang Province, China) ,  Wang Chenggui (Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, West Xueyuan Road 109#, Wenzhou 325027, Zhejiang Province, PR China) ,  Wang Chenggui (Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, Zhejiang Province, China) ,  Teng Wangsiyuan (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Teng Wangsiyuan (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Teng Wangsiyuan (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Xing Hongyuan (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Xing Hongyuan (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Xing Hongyuan (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Wang Fangqian (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Wang Fangqian (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Wang Fangqian (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Yinwang Eloy (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Yinwang Eloy (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Yinwang Eloy (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Sun Hangxiang (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Sun Hangxiang (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Sun Hangxiang (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Wu Yan (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Wu Yan (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Wu Yan (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Yu Chengcheng (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Yu Chengcheng (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Yu Chengcheng (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Chai Xupeng (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Chai Xupeng (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Chai Xupeng (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Qian Zhiyong (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, PR China) ,  Yu Xiaohua (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Yu Xiaohua (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Yu Xiaohua (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Ye Zhaoming (Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, PR China) ,  Ye Zhaoming (Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang Province, PR China) ,  Ye Zhaoming (Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang Province, PR China) ,  Wang Xiangyang (Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, West Xueyuan Road 109#, Wenzhou 325027, Zhejiang Province, PR China) ,  Wang Xiangyang (Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, Zhejiang Province, China)
資料名： Chemical Engineering Journal  (Chemical Engineering Journal)
巻： 433  号： P1  ページ： Null  発行年： 2022年 
JST資料番号： D0723A  ISSN： 1385-8947  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 脊髄損傷(SCI)の二次損傷中の炎症の持続性は,脊髄の乏しい再生能力の根本原因として同定されている。M2ミクログリア由来細胞外小胞(EVs)は,神経再生と同様に炎症緩和に対する治療的可能性を示し,しかしながら,SCI治療におけるそれらの応用は,不良な蓄積,短い保持,および病変組織における制御放出の欠如のような問題のために制限される。ここでは,新規M2誘導EVsデリバリー感熱ヒドロゲル系を,EVsの治療能と脊髄損傷に対するヒドロゲルの反応性の両方を利用して確立した。EVsをIL-4分極型2BV2細胞から単離し,ポリ(d,l-ラクチド)-ポリ(エチレングリコール)-ポリ(D,L-ラクチド)PLELヒドロゲルに組み込み,熱感受性,抗炎症および抗アポトーシスの特性を有する注射可能なヒドロゲルを開発した。PLEL感熱性ヒドロゲルは,注射中の損傷組織への接近を得るための十分な流動性を提供するだけでなく,ヒドロゲルが体温で急速に起こった時に,ヒドロゲルが展開されると,デリバリーカーゴの効果的な保持および制御放出も可能にした。in vivo結果は,PLEL/EVsヒドロゲルが,BBBスコアの上昇および瘢痕領域の低下により証明されたSCI後の運動神経機能回復を改善することを示した。機構研究は,EVの持続的放出が,EVs誘導M2分極を介して局所炎症を減弱し,Bcl-2経路を介したニューロンのアポトーシスを阻害し,脊髄を保護することを示した。したがって,このEVs負荷感熱ヒドロゲルアプローチは,抗炎症および神経防御機構の両方を介してSCI療法を改善する微小環境応答および非侵襲的経路を提供する。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 分極 ,  ニューロン ,  運動神経 ,  炎症 ,  消炎作用 ,  *脊髄 ,  体温 ,  *ヒドロゲル ,  瘢痕 ,  *脊髄損傷 ,  インターロイキン4 ,  アポトーシス ,  神経再生 ,  ミクログリア
準シソーラス用語： 微小環境 , 【Automatic Indexing@JST】

著者キーワード (5件)： M2ミクログリア ,  免疫調節 ,  細胞外小胞 ,  熱感受性ヒドロゲル ,  脊髄損傷

分類 (3件)： 多糖類  (CF10054P) ,  固形製剤  (GY02030V) ,  注射剤,眼科用製剤  (GY02050R)

タイトルに関連する用語 (8件)： 免疫調節 ,  細胞外小胞 ,  注入 ,  温度感受性 ,  ヒドロゲル ,  脊髄損傷 ,  運動機能回復 ,  増強