文献

J-GLOBAL ID：202202285367432972   整理番号：22A0323945

細胞亜集団変化とFGF7活性化は単一細胞解析により明らかにされた3D微小環境における腱幹/前駆細胞の機能を調節する【JST・京大機械翻訳】

Cell-subpopulation alteration and FGF7 activation regulate the function of tendon stem/progenitor cells in 3D microenvironment revealed by single-cell analysis

著者 (62件)： Zhang Hong (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Hong (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Hong (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Hong (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Chen Yangwu (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Yangwu (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Yangwu (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Yangwu (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Fan Chunmei (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Fan Chunmei (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Fan Chunmei (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Fan Chunmei (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Liu Richun (Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning, China) ,  Huang Jiayun (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Huang Jiayun (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Huang Jiayun (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Huang Jiayun (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Zhang Yanjie (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Yanjie (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Yanjie (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Yanjie (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Tang Chenqi (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Tang Chenqi (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Tang Chenqi (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Tang Chenqi (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Zhou Bo (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhou Bo (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhou Bo (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhou Bo (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Chen Xiaoyi (Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning, China) ,  Ju Wei (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Ju Wei (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Ju Wei (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Ju Wei (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Zhao Yanyan (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhao Yanyan (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhao Yanyan (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhao Yanyan (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Han Jie (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Han Jie (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Han Jie (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Han Jie (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Wu Peishan (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Shichen (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Shichen (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Shichen (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Zhang Shichen (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Shen Weiliang (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Shen Weiliang (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Shen Weiliang (Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Yin Zi (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Yin Zi (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Yin Zi (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Chen Xiao (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Xiao (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Xiao (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Chen Xiao (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China) ,  Ouyang Hongwei (Dr. Li Dak Sum-Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China) ,  Ouyang Hongwei (Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, China) ,  Ouyang Hongwei (Zhejiang University-University of Edinburgh Institute & School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Ouyang Hongwei (Department of Sports Medicine, Zhejiang University School of Medicine, Hangzhou, China) ,  Ouyang Hongwei (China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China)
資料名： Biomaterials  (Biomaterials)
巻： 280  ページ： Null  発行年： 2022年 
JST資料番号： C0964B  ISSN： 0142-9612  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： 3次元(3D)微小環境は,2次元(2D)微小環境と比較して,幹細胞維持および機能に対する天然微小環境をより正確に複製した。しかしながら,3D微小環境が幹細胞機能を調節する分子機構は,単一細胞レベルではほとんど検討されていない。ここでは,単一細胞分析と機能分析を用い,hTSPCの膨張と機能維持のため開発した,系統的に3Dゼラチンマイクロキャリアー培養系に基づく3D微小環境に対し,全ての細胞サブポピュレーションが反応しないことを見出した。3D微小環境は,ICAM1+ITGB8+およびFGF7+CYGB+亜集団の割合を改善することにより,ヒト腱幹/前駆細胞(hTSPC)の細胞亜集団分布を変化させる。また,2つの亜集団における活性化FGF7シグナル伝達は,細胞-細胞相互作用を通してhTSPCの10発生増強に関与することを明らかにした。特異的細胞サブポピュレーション構造を有する3Dニッチで培養したhTSPCは,in vitroとin vivoの両方で優れた幹細胞特性と機能を示した。まとめると,3D微小環境は重要な細胞亜集団を調節することにより幹細胞機能を調節することができ,腱再生と腱再生のための新規調節因子としてFGF7を同定した。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 相互作用 ,  *細胞 ,  ヒト ,  *環境 ,  *腱 ,  二次元 ,  機能分析 ,  *三次元 ,  幹細胞 ,  細胞情報伝達 ,  in vitro実験
準シソーラス用語： 分子機構 ,  調節因子 ,  *微小環境 ,  *単一細胞 ,  *前駆細胞 , 【Automatic Indexing@JST】

著者キーワード (6件)： 3D微小環境 ,  細胞亜集団 ,  FGF7 ,  hTSPC ,  テノジェネシス ,  腱再生

分類 (2件)： 運動器系の基礎医学  (GG01020B) ,  医用素材  (GA05040H)

タイトルに関連する用語 (11件)： 細胞 ,  亜集団 ,  FGF7 ,  活性化 ,  単一細胞解析 ,  3D ,  微小環境 ,  腱 ,  幹 ,  前駆細胞 ,  調節