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J-GLOBAL ID：202202288838357589   整理番号：22A1048982

ブタ流行性下痢ウイルスの転写調節配列の転写調節効果の評価【JST・京大機械翻訳】

Evaluation of the transcriptional regulatory efficacy of transcription regulatory sequences of porcine epidemic diarrhea virus

著者 (31件)： Peng Qi (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Peng Qi (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Peng Qi (Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou 225009, China) ,  Zhang Xue (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Zhang Xue (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Zhang Xue (Key Laboratory of Animal Disease Diagnosis & Immunology, College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, China) ,  Fan Baochao (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Fan Baochao (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Fan Baochao (Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou 225009, China) ,  Li Yunchuan (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Li Yunchuan (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Zhao Shuqing (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Zhao Shuqing (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Zhao Shuqing (College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210094, China) ,  Guo Weilu (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Guo Weilu (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Guo Weilu (School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210094, China) ,  He Wenlong (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  He Wenlong (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  He Wenlong (College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China) ,  Zhao Yongxiang (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Zhao Yongxiang (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Ni Yanxiu (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Ni Yanxiu (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Liu Maojun (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Liu Maojun (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Fei Rongmei (Key Laboratory of Animal Disease Diagnosis & Immunology, College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, China) ,  Li Bin (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China) ,  Li Bin (Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China) ,  Li Bin (Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou 225009, China) ,  Li Bin (College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China)
資料名： Veterinary Microbiology  (Veterinary Microbiology)
巻： 267  ページ： Null  発行年： 2022年 
JST資料番号： A1255A  ISSN： 0378-1135  資料種別： 逐次刊行物 (A)
記事区分： 短報  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： ブタ伝染性下痢ウイルス(PEDV)は,子豚において重度の水性下痢と高い死亡率を引き起こす高度に伝染性の腸病原性コロナウイルスである。PEDVの遺伝子発現と複製の調節における転写調節配列(TRS)の役割を調べるために,PEDVの異なるTRSの制御下の増強緑色蛍光蛋白質(EGFP)遺伝子を,逆遺伝子系を用いてPEDVゲノムのN遺伝子と3′UTRの間に挿入した。異なるキメラPEDVからのEGFP発現を各TRSに対して分析した。PEDVのすべての構造的およびアクセサリー蛋白質遺伝子のTRSは,異なるレベルでEGFP発現を正に調節し,M蛋白質遺伝子のTRSはEGFPの最高レベルを産生した。さらに,これは外因性遺伝子がPEDVのN遺伝子と3’UTRの間に挿入され,EGFP挿入がPEDV複製に影響を及ぼさないことを示す最初の研究である。まとめると,著者らの研究は,PEDVの遺伝子発現と複製に関するPEDV TRSの情報を強化した。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 遺伝子 ,  死亡率 ,  タンパク質 ,  *ブタ ,  ゲノム ,  キメラ ,  ウイルスタンパク質 ,  病原性 ,  *コロナウイルス科 ,  伝染性下痢症 ,  遺伝子発現
準シソーラス用語： コロナウイルス ,  EGFP ,  アクセサリー蛋白質 ,  M蛋白質 ,  *転写調節 ,  *ブタ流行性下痢ウイルス ,  子豚 , 【Automatic Indexing@JST】

著者キーワード (4件)： PEDV ,  TRS ,  逆遺伝学 ,  EGFP

分類 (2件)： ウイルスによる動物の伝染病  (FE02024R) ,  豚  (FD02040U)

タイトルに関連する用語 (5件)： ブタ流行性下痢ウイルス ,  転写調節 ,  転写 ,  調節効果 ,  評価