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J-GLOBAL ID：202202289312806784   整理番号：22A0477836

Mycobacterium tuberculosis H37Rvの深部N-テルミノミクスは広範なコード化遺伝子をコードする【JST・京大機械翻訳】

Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct annotated encoding genes

著者 (22件)： Shi Jiahui (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Shi Jiahui (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, School of Life Sciences and Institute of Life Science and Green Development, Hebei University, Baoding 071002, China) ,  Meng Shuhong (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Meng Shuhong (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, School of Life Sciences and Institute of Life Science and Green Development, Hebei University, Baoding 071002, China) ,  Wan Li (State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, Chi...) ,  Zhang Zhenpeng (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Jiang Songhao (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Jiang Songhao (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, School of Life Sciences and Institute of Life Science and Green Development, Hebei University, Baoding 071002, China) ,  Zhu Huiming (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Zhu Huiming (Guizhou University, School of Medicine, Guiyang 550025, China) ,  Dai Erhei (The Fifth Hospital of Shijiazhuang, North China University of Science and Technology, Shijiazhuang 050021, China) ,  Chang Lei (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Gao Huiying (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Wan Kanglin (State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, Chi...) ,  Zhang Liqun (Beijing Chest Hospital, Capital Medical University, Beijing 101149, China) ,  Zhao Xiuqin (State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, Chi...) ,  Liu Haican (State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, Chi...) ,  Lyu Zhitang (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, School of Life Sciences and Institute of Life Science and Green Development, Hebei University, Baoding 071002, China) ,  Zhang Yao (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Xu Ping (State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China) ,  Xu Ping (Key Laboratory of Microbial Diversity Research and Application of Hebei Province, School of Life Sciences and Institute of Life Science and Green Development, Hebei University, Baoding 071002, China) ,  Xu Ping (Guizhou University, School of Medicine, Guiyang 550025, China)
資料名： Genomics  (Genomics)
巻： 114  号： 1  ページ： 292-304  発行年： 2022年 
JST資料番号： T0422A  ISSN： 0888-7543  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： オランダ (NLD)  言語： 英語 (EN)

抄録/ポイント： 結核菌(MTB)は,結核(TB)の重篤な原因菌である。H37Rvでは,M.tuberculosisの型株は1998年に配列決定されたが,コード遺伝子のアノテーションエラーは数百の論文で頻繁に報告されている。この現象は遺伝子の5′末端で特に厳しい。ここでは,M.tuberculosis H37Rvに対するStageTip分離戦略と組み合わせたTMPP[(N-スクシンイミジルオキシカルボニルメチル)トリス(2,4,6-トリメトキシフェニル)ホスホニウムブロミド]標識)を適用し,その注釈コード遺伝子のN末端開始部位を特性化した。全部で1047の蛋白質は,2625の質量分析計(MS)配列蛋白質から2058のTMPP標識N末端ペプチドで同定された。比較ゲノム解析は,H37Rvにおける43の蛋白質N末端の再注釈とMycobacteriaceaeにおける762の蛋白質を可能にした。すべての改訂N末端開始部位は,以前のN末端開始コドン,特にATGの過剰注釈により,注釈遺伝子の5’-UTRに分布した。さらに,+2フレームにおける注釈遺伝子Rv1078とは異なる+3フレームにおける新規遺伝子Rv1078Aを同定し,検証した。まとめると,著者らの知見は,生物学的研究に関する将来の研究を助けることができるMycobacteriaceaeからのH37Rvおよび他の種のN末端のより良い理解に寄与する。Copyright 2022 Elsevier B.V., Amsterdam. All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 質量分析計 ,  ペプチド ,  *遺伝子 ,  結核 ,  タンパク質 ,  *結核菌 ,  *Mycobacterium ,  キャラクタリゼーション ,  開始コドン ,  アノテーション
準シソーラス用語： 比較ゲノム解析 ,  Mycobacteriaceae ,  アミノ末端 ,  起因菌 , 【Automatic Indexing@JST】

著者キーワード (4件)： Mycobacterium tuberculosis ,  N-テルミノミクス ,  TMPP ,  StageTip

分類 (4件)： 微生物の生化学  (EG03020N) ,  酵素生理  (EG03052C) ,  酵素一般  (EB09010D) ,  遺伝子の構造と化学  (EC02020U)

タイトルに関連する用語 (3件)： Mycobacterium ,  深部 ,  遺伝子