Rchr
J-GLOBAL ID:200901006681087385
Update date: Feb. 01, 2024
Nakai Tadashi
ナカイ タダシ | Nakai Tadashi
Affiliation and department:
Job title:
Professor
Homepage URL (1):
https://www.researchgate.net/profile/Tadashi_Nakai
Research field (4):
Applied microbiology
, Applied biochemistry
, Functional biochemistry
, Structural biochemistry
Research keywords (14):
radical SAM superfamily
, intra-protein thioether bond
, multienzyme complex
, iron-sulfur cluster
, reaction mechanism
, molecular biology
, genetic engineering
, coenzyme
, enzyme
, post-translational modification
, mass spectrometry
, X-ray crystallography
, structural biology
, biochemistry
Research theme for competitive and other funds (13):
- 2022 - 2025 Mechanistic analysis and its optimization of bioactive cyclic peptides constructed by a radical cross-linking enzyme
- 2022 - 2025 Elucidation of the biogenesis mechanism of amine dehydrogenase by development of a low-cytotoxicity genome editing technique for bacteria
- 2022 - 2023 Elucidation of the biogenesis mechanism of amine dehydrogenase by development of a low-cytotoxicity genome editing technique for Paracoccus denitrificans
- 2018 - 2022 Mechanism of intra-peptidyl thioether bond forming enzyme and its application to development of bioactive polycyclic peptides
- 2016 - 2020 Elucidation of enzyme catalytic mechanism regulating transition state by linking conformational change and fluctuations of the active site
- 2016 - 2019 Mechanism of multi-step posttranslational modification involved in biogenesis of amine dehydrogenase and its application to development of bioactive polycyclic peptides
- 2015 - 2018 Reaction mechanism of novel tryptophan-hydroxylating enzyme involving in quinone cofactor biogenesis
- 2011 - 2013 Biogenesis process of quinohemoprotein aminedehydrogenase accompanying multi-step posttranslational modification reactions
- 2011 - 2012 Catalytic mechanism of a novel intra-peptidyl bond forming enzyme
- 2007 - 2009 Crystallographic studies of RNA-editing core complexes of editosome from the sleeping sickness parasite Trypanosoma brucei
- 2003 - 2005 Structure and mechanism of glycine cleavage system multienzyme complex from Thermus thermophilus
- 2000 - 2003 X-ray structural analysis of glycine cleavage system multienzyme complex from Thermus thermophilus
- 2001 - 2002 X-ray structural analysis of glycine cleavage system multienzyme complex from Thermus thermophilus
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Papers (32):
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Nakai T, Okajima T. [Structure and function of a novel flavoenzyme involved in quinone cofactor biogenesis] (Article in Japanese). Seikagaku. 2022. 94. 1. 92-96
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Tadashi Nakai, Katsuyuki Tanizawa, Toshihide Okajima. Eight genes are necessary and sufficient for biogenesis of quinohemoprotein amine dehydrogenase. Bioscience, biotechnology, and biochemistry. 2021
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Toshinori Oozeki, Tadashi Nakai, Kazuki Kozakai, Kazuki Okamoto, Shun'ichi Kuroda, Kazuo Kobayashi, Katsuyuki Tanizawa, Toshihide Okajima. Functional and structural characterization of a flavoprotein monooxygenase essential for biogenesis of tryptophylquinone cofactor. Nature Communications. 2021. 12. 1. 933
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Masaharu Somiya, Qiushi Liu, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Kazuyuki Kuroki, Keiji Ueda, Shun'ichi Kuroda. Cellular uptake of hepatitis B virus envelope L particles is independent of sodium taurocholate cotransporting polypeptide, but dependent on heparan sulfate proteoglycan. VIROLOGY. 2016. 497. 23-32
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Tadashi Nakai, Katsuyuki Tanizawa, Toshihide Okajima. Novel radical SAM enzyme forming intrapeptidyl crosslinks. Seikagaku. 2016. 88. 4. 506-510
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MISC (14):
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Tadashi Nakai. Development of a Fluorescent Protein-based Laboratory Course for Life Science Students. Bull. Hiroshima Inst. Tech. Education. 2020. 19. 61-69
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Toshinori Oozeki, Kazuki Kozakai, Tadashi Nakai, Katsuyuki Tanizawa, Toshihide Okajima. Sequence Specificity for Peptide Substrates in Thioether Crosslinking Reaction Catalyzed by Radical SAM Enzyme QhpD. Protein Science. 2019. 28. S1. 169-170
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Oozeki T, Nakai T, Tanizawa K, Okajima T. Role of flavin-containing enzyme in the post-translational modification of quinoheme protein amine dehydrogenase. 2018
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中井忠志, 小林一雄, 高橋康弘, 堀洋, 鍔木基成, 外山博英, 谷澤克行, 谷澤克行, 岡島俊英. ペプチドの翻訳後修飾を触媒するラジカルSAMスーパーファミリー酵素. 日本生化学会大会(Web). 2015. 88th
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Toshihide OKAJIMA, Shougo KIKUKAWA, Akio HAMAGUCHI, Tadashi NAKAI, Katsuyuki TANIZAWA. Catalytic Mechanism of Copper Amine Oxidase: X-Ray Crystal Structure of the Intermediates Formed in the Oxidative-Half Reaction. 2011
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Lectures and oral presentations (30):
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Heterologous expression of a PET hydrolase confers plastic degrading capacity on Paracoccus denitrificans
(The 2022 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry 2022)
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Promoter analysis of Pden_1710, a gene neighboring the quinohemoprotein amine dehydrogenase operon
(The 2020 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry 2020)
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Functional analysis of serine proteinase involved in biosynthesis of active-site subunit of quinohemoprotein amine dehydrogenase
(The 2019 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry 2019)
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Functions of the enzyme complex involved in post-translational modification of quinohemoprotein amine dehydrogenase
(The 2018 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry 2018)
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Cyclic peptide formation by a peptide thioether crosslink-forming enzyme QhpD: Substrate peptide length and sequence allowed for the crosslink
(The 2018 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry 2018)
more...
Education (3):
- 1997 - 2000 Osaka City University Graduate School of Science Department of Chemistry
- 1996 - 1997 Osaka City University Graduate School of Science Department of Chemistry
- 1992 - 1996 Osaka City University Faculty of Science Department of Chemistry
Professional career (1):
- Ph.D. in Chemistry (Osaka City University)
Work history (10):
- 2023/04 - 現在 Osaka University The Institute of Scientific and Industrial Research Guest Professor
- 2022/10 - 現在 Hiroshima Institute of Technology Department of Food Sciences and Biotechnology, Faculty of Life Sciences Professor
- 2017/04 - 2023/03 Osaka University Institute of Scientific and Industrial Research Guest Associate Professor
- 2017/04 - 2022/09 Hiroshima Institute of Technology Department of Food Sciences and Biotechnology, Faculty of Life Sciences Associate Professor
- 2009/06 - 2017/03 Osaka University Graduate School of Science Assistant Professor
- 2009/06 - 2017/03 Osaka University Institute of Scientific and Industrial Research Assistant Professor
- 2007/04 - 2009/04 University of Washington Department of Biochemistry (Prof. Wim G.J. Hol group) JSPS Postdoctoral Fellow for Research Abroad
- 2005/04 - 2007/03 RIKEN Harima Institute/SPring-8 Advanced Protein Crystallography Research Group (Dr. Nobuo Kamiya group) Research Scientis
- 2003/04 - 2005/03 Osaka University Graduate School of Science (Prof. Seiki Kuramitsu group) JSPS Research Fellow
- 2000/04 - 2003/03 RIKEN Harima Institute/SPring-8 Division of Bio-Crystallography Technology (Dr. Nobuo Kamiya group) Special Postdoctoral Researcher
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Committee career (1):
- 2010 - 2010 日本結晶学会 平成22年度年会 実行委員
Awards (2):
- 2012/01 - Institute of Scientific and Industrial Research, Osaka University Best Poster Award (The 15th SANKEN International Symposium 2012 / The 10th SANKEN Nanotechnology Symposium) An unusual subtilisin-like serine protease is essential for biogenesis of quinohemoprotein amine dehydrogenase
- 2009/10 - Japanese Biochemical Society JB Best Paper Award Structural bases for the specific interactions between the E2 and E3 components of the Thermus thermophilus 2-oxo acid dehydrogenase complexes
Association Membership(s) (3):
Japanese Biochemical Society
, Crystallographic Society of Japan
, Japan Society for Bioscience, Biotechnology and Agrochemistry
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