KIMURA Shigenobu

キムラシゲノブ | KIMURA Shigenobu

Affiliation and department：
Job title： Specially appointed professor

Research field (3)： Environmental load reduction/restoration technology , Structural biochemistry , Functional biochemistry

Research keywords (1)： protein, protein engineering, electron transfer, flavoenzyme, expression system, bioremediation

Research theme for competitive and other funds (8)：

2021 - 2024 シアノバクテリアを用いた生物学的環境修復のための細胞内電子供給経路の機能解明
2015 - 2019 シアノバクテリアの細胞内電子供給系路の解明と新規芳香族化合物分解株の創成
2013 - 2017 Elucidation of catalytic mechanism of NADH-cytochrome b5 reductase by time-resolved X-ray and neutron analyses
2013 - 2016 シアノバクテリアを利用した膜タンパク質大量生産系の構築
2009 - 2013 貧栄養環境下で芳香族環境汚染物質を分解する新規光合成微生物の改良と展開

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Papers (61)：

Takaaki Suzuki, Akito Nishizawa, Masashi Kikuchi, Chihiro Nonaka, Mariko Komuro, Miki Nakayama, Yasuhiro Kashino, Masao Fukuda, Shigenobu Kimura. Biphenyl degradation by recombinant photosynthetic cyanobacterium Synechocystis sp. PCC6803 in an oligotrophic environment using unphysiological electron transfer. Biochem. J. 2019. 476. 23. 3615-3630
Yu Hirano, Shigenobu Kimura, Taro Tamada. High-resolution crystal structures of the solubilized domain of porcine cytochrome b₅. Acta Crystallographica Section D Biol. Crystallogr. 2015. 71. 1572-1581-1581
Gen Okano, Shukuro Igarashi, Yuhei Yamamoto, Shotaro Saito, Yoshitaka Takagai, Takao Ohtomo, Shigenobu Kimura, Osamu Ohno, Yoshio Oka. HPLC-spectrophotometric detection of trace heavy metals via 'cascade' separation and concentration. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY. 2015. 95. 2. 135-144
Akito Nishizawa, Ayaka Harada, Miki Senda, Yuka Tachihara, Daisuke Muramatsu, Shinya Kishigami, Shigemasa Mori, Keisuke Sugiyama, Toshiya Senda, Shigenobu Kimura. Complete pyridine-nucleotide-specific conversion of an NADH-dependent ferredoxin reductase. BIOCHEMICAL JOURNAL. 2014. 462. 2. 257-265
Mitsugu Yamada, Taro Tamada, Kazuki Takeda, Fumiko Matsumoto, Hiraku Ohno, Masayuki Kosugi, Kiyofumi Takaba, Yoshinari Shoyama, Shigenobu Kimura, Ryota Kuroki, et al. Elucidations of the Catalytic Cycle of NADH-Cytochrome b(5) Reductase by X-ray Crystallography: New Insights into Regulation of Efficient Electron Transfer. JOURNAL OF MOLECULAR BIOLOGY. 2013. 425. 22. 4295-4306

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MISC (17)：

平野優, 平野優, 栗原和男, 日下勝弘, オスターマンアンドレアス, 木村成伸, 三木邦夫, 玉田太郎. NADH-シトクロムb5還元酵素反応系の高分解能構造解析. 日本細胞生物学会大会(Web). 2019. 71st. ROMBUNNO.2P-036 (WEB ONLY)
平野優, 平野優, 栗原和男, 日下勝弘, OSTERMANN Andreas, 木村成伸, 三木邦夫, 玉田太郎. NADHシトクロムb₅還元酵素の高分解能構造解析. 日本結晶学会年会講演要旨集. 2018. 2018. 97
平野優, 平野優, 栗原和男, 日下勝弘, 木村成伸, 三木邦夫, 玉田太郎. 酸化型NADHシトクロムb5還元酵素の中性子構造解析. 日本蛋白質科学会年会プログラム・要旨集. 2018. 18th. 30
平野優, 栗原和男, 日下勝弘, 木村成伸, 三木邦夫, 玉田太郎. 酸化型NADHシトクロムb₅還元酵素の中性子構造解析. 日本結晶学会年会講演要旨集. 2017. 2017. 104
平野優, 平野優, 栗原和男, 日下勝弘, 木村成伸, 三木邦夫, 玉田太郎. NADHシトケロムb₅還元酵素の中性子構造解析. 日本中性子科学会年会講演概要集. 2017. 17th. 75

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Patents (2)：

芳香族化合物分解能を有する光合成生物および芳香族化合物の分解方法
芳香族化合物分解能を有する光合成生物および芳香族化合物の分解方法

Books (3)：

貧栄養下で芳香族化合物を分解できる新規光合成微生物
ケミカルエンジニヤリング 2009
Molecular Mechanism of the Redox-dependent Interaction betweenNADH-dependent Ferredoxin Reductase and Rieske-type Ferredoxin
J. Cryst. Soc. Jpn. 2008
Molecular Mechanism of the Redox-dependent Interaction between NADH-dependent Ferredoxin Reductase and Rieske-type [2Fe-2S] Ferredoxin
化学と生物 2008

Lectures and oral presentations (195)：

Effect of the FAD2-binding site mutations on the FAD-binding of disulfide-linked disulfide oxidoreductase
(The 97th Annual Meeting of Japanese Biochemical Society 2024)
FAD-binding to apo-form of diflavin-linked disulfide oxidoreductase
(The 24th Annual Meeting of the Protein Science Society of Japan 2024)
1分子 FAD結合型DDOR作製の試みとapo-DDORへのFAD結合過程の解析
(第34回日本化学会関東支部茨城地区研究交流会 2023)
1分子FAD結合型ジフラビン結合ジスルフィド酸化還元酵素作製の試み
(第96回日本生化学会大会 2023)
X-ray crystal structure analysis of di-flavin-binding disulfide oxidoreductase from binding to apo-form of <I>Synechocystis</I> sp. PCC6803,product in solution
(2022)

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Education (2)：

1982 - 1984 Tohoku University 理学研究科化学第二専攻博士課程前期2年の過程
- 1984 Graduate School of Tohoku University Department of Science Chemistry

Professional career (1)：

Ph.D. (Tohoku University)

Work history (9)：

2024/06 - 現在 Ibaraki University
2024/04 - 現在 Graduate School of Science and Engineering, Ibaraki University Specially Appointed Professor
2005/04 - 2024/03 Professor, Graduate School of Science and Engineering, Ibaraki University
2004/04 - 2005/03 Associate Professor, Graduate School of Life Science, University of Hyogo
2002/04 - 2004/03 Associate Professor, Department of Life Science, Graduate School of Science, Himeji Institute of Technology

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Committee career (2)：

2014/10/01 - 日本生化学会評議員
2012/10 - 2013/09 The Japanese Biochemical Society 代議員

Awards (2)：

2011/09 - Japan Society for the Promotion of Science 平成23年度科研費審査委員表彰科研費の第1段審査(書面審査)の検証結果に基づき、模範となる審査意見を付した審査委員。
2004 - 日本生化学会 JB論文賞 2004年(第12回)

Association Membership(s) (4)：

The Japanese Biochemical Society , American Society of Biochemistry and Molecular Biology (ASBMB) , The Molecular Biology Society of Japan , Pritein Science Society of Japan

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