2013 - 2015 Development of fundamental technology to create E. coli carrying human-type glycosylation
Papers (54)：
Takao Ohashi, Yurika Mabira, Yutaro Mitsuyoshi, Hiroyuki Kajiura, Ryo Misaki, Takeshi Ishimizu, Kazuhito Fujiyama. Expression of an endo-rhamnogalacturonase from Aspergillus aculeatus enhances release of Arabidopsis transparent mucilage. Journal of bioscience and bioengineering. 2024
Sato K, Yumioka H, Isoyama J, Dohi K, Yamanaka A, Ohashi T, Misaki R, Fujiyama K. Man5GlcNAc2 structure by the combination of N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1. J Biosci Bioeng. 2023. 136. 2. 136-141
Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama. High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves. Plant biotechnology (Tokyo, Japan). 2022. 39. 3. 291-301
Takamasa Fukunaga, Takao Ohashi, Yutaka Tanaka, Tomoki Yoshimatsu, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa. Galactosylation of cell-surface glycoprotein required for hyphal growth and cell wall integrity in Schizosaccharomyces japonicus. Journal of bioscience and bioengineering. 2022. 134. 5. 384-392
Misaki R, Iwasaki M, Takechi H, Yamano-Adachi N, Ohashi T, Kajiura H, Fujiyama K. Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter. Cytotechnology. 2022. 74. 1. 163-179
