Hagiwara Yoshinori

Affiliation and department：
Homepage URL (1)： http://www.cc.kurume-nct.ac.jp/~hagiwara/world/

Research field (3)： Structural biochemistry , Functional biochemistry , Plants: molecular biology and physiology

Research keywords (5)：光合成アンテナ , 光合成色素 , タンパク質工学 , X線結晶構造解析 , 構造生物学

Research theme for competitive and other funds (2)：

2020 - 2023 Construction of bilin reductase library, promotes next-generation optogenetics.
2012 - 2014 Structural and functional analysis of a novel iron-sulpfur protein involved in the intracellular assembly of light-harvesting antenna

Papers (17)：

Tatsuya Joutsuka, Ryota Nanasawa, Keisuke Igarashi, Kazuki Horie, Masakazu Sugishima, Yoshinori Hagiwara, Kei Wada, Keiichi Fukuyama, Naomine Yano, Seiji Mori, et al. Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states. Journal of Biological Chemistry. 2023. 299. 1. 102763-102763
Ishi-I T, Kawai K, Shirai Y, Kitahara I, Hagiwara Y. Amphiphilic triphenylamine-benzothiadiazole dyes: preparation, fluorescence and aggregation behavior, and enzyme fluorescence detection. Photochemical and Photobiological Sciences. 2019. 18. 1447-1460
Igarashi K, Hagiwara Y, Sugishima M, Wada K, Fukuyama K, Ikeda A, Yano N, Kusaka K, Ostermann A, Unno M. Crystal Growth of a Bilin Reductase PcyA I86D Mutant-Substrate Complex for Neutron Crystallography. Crystal Growth and Design. 2018. 18. 5174-5181
Haruna Takao, Kei Hirabayashi, Yuki Nishigaya, Haruna Kouriki, Tetsuko Nakaniwa, Yoshinori Hagiwara, Jiro Harada, Hideaki Sato, Toshimasa Yamazaki, Yoichi Sakakibara, et al. A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity. NATURE COMMUNICATIONS. 2017. 8. 14397
Yoshinori Hagiwara, Kei Wada, Teppei Irikawa, Hideaki Sato, Masaki Unno, Ken Yamamoto, Keiichi Fukuyama, Masakazu Sugishima. Atomic-resolution structure of the phycocyanobilin:ferredoxin oxidoreductase I86D mutant in complex with fully protonated biliverdin. FEBS LETTERS. 2016. 590. 19. 3425-3434

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MISC (8)：

Unno Masaki, Kusaka Katsuhiro, Tamada Taro, Sugishima Masakazu, Wada Kei, Hagiwara Yoshinori, Fukuyama Keiichi. Findings in the Neutron Crystal Structure Analysis of a Bilin Reductase PcyA Complexed with its Substrate Biliverdin. hamon. 2016. 26. 3. 130-134
Crystallization of cyanobacterial Ycf34 protein from Synechocystis sp. PCC 6803. 2015. 31. 1. 29-33
海野昌喜, 海野昌喜, 須藤久美子, 日下勝弘, 玉田太郎, 萩原義徳, 杉島正一, 和田啓, 山田太郎, 石原真樹子, et al. 中性子結晶構造解析によるフェレドキシン依存性ビリン還元酵素基質複合体の水素化状態可視化. 物構研サイエンスフェスタ要旨集. 2015. 3rd
UNNO Masaki, SUGISHIMA Masakazu, WADA Kei, HAGIWARA Yoshinori, KUSAKA Katsuhiro, TAMADA Taro, FUKUYAMA Keiichi. Two Protonation States and Structural Features of a Bilin Reductase PcyA Revealed by Neutron Crystallography. Nihon Kessho Gakkaishi. 2015. 57. 5. 297-303
中嶌裕之, 萩原義徳, 冨永洋一, 山本好和. 佐賀県黒髪山の地衣類. 久留米工業高等専門学校紀要. 2015. 30. 2. 19-23

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Lectures and oral presentations (3)：

シアノバクテリアの光捕集と光受容に関わるタンパク質の構造と機能
(日本地衣学会第14回大会一般公開シンポジウム 2015)
フェレドキシン依存性ビリン還元酵素PcyAによる光受容色素合成の構造生物学
(第87回日本生化学会大会フォーラム「ヘム・ビリン代謝の生化学と構造生物学」 2014)
Scientific and Scenic life in Germany
(Symposium on EU-Japan Collaboration in Education, Research and Exchanges 2011)

Education (2)：

- 2010 Osaka University Graduate School of Science Department of Biological Sciences
- 2004 Shizuoka University Faculty of Science

Professional career (1)：

博士(理学) (大阪大学)

Work history (7)：

2017/04 - 現在 National Institute of Technology, Kurume College Department of Biochemistry and Applied Chemistry
2017/07 - 2017/08 Queens College of the City University of New York Department of Chemistry and Biochemistry Department Visiting Researcher
2016/04 - National Institute of Technology, Kurume College Department of Biochemistry and Applied Chemistry
2012/01 - National Institute of Technology, Kurume College Department of Biochemistry and Applied Chemistry
2010/05 - Ruhr-University Bochum Department of Biology and Biotechnology Postdoctoral fellow

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Association Membership(s) (3)：

THE JAPANESE SOCIETY FOR LICHENOLOGY , THE CRYSTALLOGRAPHIC SOCIETY OF JAPAN , PROTEIN SCIENCE SOCIETY OF JAPAN

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