Research field (3):
Plant genetics and breeding
, Evolutionary biology
, Systems genomics
Research theme for competitive and other funds (4):
2017 - 2021 標的DNAのあらゆる塩基を自在に直接変換できる人工酵素技術の創出
2016 - 2018 半合理的なゲノム進化デザインを可能とする自己進化型ゲノム編集システムの創成
2017 - 【OPERA】ゲノム編集による革新的な有用細胞・生物作成技術の創出
2014 - 2016 DNA 塩基置換酵素の"逆進化"誘導によるDNAを切らないゲノム編集ツールの開発
Papers (61):
Soh Ishiguro, Kana Ishida, Rina C. Sakata, Hideto Mori, Mamoru Takana, Samuel King, Omar Bashth, Minori Ichiraku, Nanami Masuyama, Ren Takimoto, et al. A multi-kingdom genetic barcoding system for precise target clone isolation. 2023
Sachiko Kashojiya, Yu Lu, Mariko Takayama, Hiroki Komatsu, Luyen Hieu Thi Minh, Keiji Nishida, Kenta Shirasawa, Kenji Miura, Satoko Nonaka, Jun-Ichiro Masuda, et al. Modification of tomato breeding traits and plant hormone signaling by target-AID, the genome-editing system inducing efficient nucleotide substitution. Horticulture research. 2022
Johan Hunziker, Keiji Nishida, Akihiko Kondo, Tohru Ariizumi, Hiroshi Ezura. Phenotypic Characterization of High Carotenoid Tomato Mutants Generated by the Target-AID Base-Editing Technology. Frontiers in plant science. 2022. 13. 848560-848560
Kohei Kawaguchi, Rie Takei-Hoshi, Ikue Yoshikawa, Keiji Nishida, Makoto Kobayashi, Miyako Kusano, Yu Lu, Tohru Ariizumi, Hiroshi Ezura, Shungo Otagaki, et al. Functional disruption of cell wall invertase inhibitor by genome editing increases sugar content of tomato fruit without decrease fruit weight. Scientific Reports. 2021. 11. 1
Keiji Nishida, Akihiko Kondo. Transversion Expansion of Base Editing. The CRISPR journal. 2021. 4. 4. 462-463
Kenta Katayama, Jun Teramoto, Akihiko Kondo, Keiji Nishida. A COMPUTATIONAL TOOL TO DESIGN UNIQUE GUIDE RNAS FOR TARGET-AID MEDIATED GENOME-WIDE BASE EDITING. Frontiers in Genome Engineering 2019. 2019