Sakamoto Masayuki

Affiliation and department：
Homepage URL (1)： https://optoneuro.weebly.com/

Research field (3)： Bio-, chemical, and soft-matter physics , Neuroscience - general , Molecular biology

Research keywords (7)：膜電位イメージング , カルシウムイメージング , Protein engineering , 2光子励起顕微鏡 , オプトジェネティクス , In vivo imaging , 樹状突起スパイン

Research theme for competitive and other funds (8)：

2023 - 2026 Exploring the functional significance of granule cells in the olfactory bulb using holographic techniques.
2021 - 2023 Development and application of multiphoton excitation for simultaneous multi-color measurement and manipulation of neural activity
2020 - 2023 Deciphering functional significance of newly generated neurons at postnatal stage investigated by multi-photon 4D imaging
2019 - 2021 「個性」創発メカニズム解明のためのシナプスイメージング法の開発と応用
2018 - 2020 Generation and application of transgenic mice for wide-field and multi-color calcium imaging

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Papers (24)：

Tatsushi Yokoyama, Satoshi Manita, Hiroyuki Uwamori, Mio Tajiri, Itaru Imayoshi, Sho Yagishita, Masanori Murayama, Kazuo Kitamura, Masayuki Sakamoto. A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nature Methods. 2024. 21. 5. 897-907
Liuqing Wang, Jia Li, Yiping Wang, Ziyi Zhong, Yuqing Wang, Rui Huang, Bingwei Zhang, Panpan Liu, Erkejiang Ye, Ruotong Cao, et al. Genome-wide CRISPR screen identifies AC9 as a key regulator of ER Ca²⁺homeostasis involved in neuronal differentiation. bioRxiv. 2024
Shiho Kawanishi, Keiichi Kojima, Atsushi Shibukawa, Masayuki Sakamoto, Yuki Sudo. Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging. ACS Omega. 2023. 8. 5. 4826-4834
Masayuki Sakamoto, Keisuke Ota, Yayoi Kondo, Michiko Okamura, Hajime Fujii, Haruhiko Bito. In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protocols. 2022. 3. 2. 101421-101421
Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, et al. A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods. 2022. 2. 100168

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MISC (5)：

坂本雅行. イメージング技術を応用した神経活動計測. 日本神経回路学会誌. 2023. 30. 4. 189-196
坂本雅行. カルシウムイメージングによる神経活動の計測. BRAIN and NERVE. 2023. 75. 2. 159-165
坂本雅行. イメージング技術を用いた神経細胞の電気的活動の計測. 生物工学会誌. 2022. 100. 7. 355-358
坂本雅行. 光技術で明らかになった樹状突起スパインの新たな機能. 実験医学 2022年5月号. 2022. 40. 8. 1274-1275
井上昌俊, 藤井哉, 坂本雅行, 尾藤晴彦. 新規高性能カルシウムセンサー分子設計から脳神経回路の情報動態解明へ. 生体の科学. 2022. 73. 5. 440-441

Lectures and oral presentations (17)：

神経活動・細胞内シグナル可視化のための蛍光プローブの開発と応用
(第5回神経計算セミナー、同志社大学大学院脳科学研究科(京田辺) 2024)
Fluorescent indicators for probing neuronal activity and biochemical signaling in vivo
(The 62nd Frontier Brain Science Seminar 2024)
神経活動可視化のための蛍光プローブの開発と応用
(第5回これからの神経回路研究の会、理化学研究所(神戸) 2023)
Fluorescent biosensors for probing neuronal activity and biochemical signaling
(Hamburg University - Kyoto University Joint Symposium “Cellular mechanisms of learning and memory”, Kyoto, Japan 2023)
神経活動可視化のための蛍光プローブの開発と応用
(第 11 回パラレル脳研究部門セミナー、東京理科大学 (柏) 2023)

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Education (3)：

2009 - 2012 京都大学大学院生命科学研究科博士後期課程
2007 - 2009 京都大学大学院生命科学研究科修士課程
2003 - 2007 Gifu Pharmaceutical University Faculty of Pharmaceutical Science Department of Manufacturing Pharmacy

Professional career (1)：

Work history (8)：

2023/04 - 現在 Kyoto University Graduate School of Biostudies Associate Professor
2020/04 - 2023/03 Kyoto University Graduate School of Biostudies Associate Professor
2019/10 - 2023/03 科学技術振興機構さきがけ研究員(兼任)
2016/04 - 2020/03 The University of Tokyo Graduate School of Medicine
2014/10 - 2016/03 Columbia University Department of Biological Sciences Postdoctoral Fellow

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Committee career (1)：

2021/03 - 現在 Frontiers in Behavioral Neuroscience Frontiers in Behavioral Neuroscience Review editor

Awards (5)：

Association Membership(s) (4)：

THE JAPANESE SOCIETY FOR NEUROCHEMISTRY , Society for Neuroscience , THE MOLECULAR BIOLOGY SOCIETY OF JAPAN , THE JAPAN NEUROSCIENCE SOCIETY

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