Horitani Masaki

ホリタニマサキ | Horitani Masaki

Affiliation and department：
Job title： Associate Professor
Other affiliations (1)：

Kagoshima University Associate Professor

Homepage URL (1)： http://horitani.ag.saga-u.ac.jp/horitanilab/index.html

Research field (5)： Other , Biophysics , Biochemistry , Structural biochemistry , Applied biochemistry

Research keywords (6)： structural biology , biophysics , enzymatic reaction , metalloprotein , electron spin resonance , biological Inorganic Chemistry

Research theme for competitive and other funds (8)：

2022 - 2025 熱帯樹木由来高活性イソプレン合成酵素の探索とハイブリッド型発酵用高機能酵素の開発
2022 - 2024 生命金属動態の解明に向けた光照射EPR技術による新しい酵素反応機構解析法の開発
2021 - 2024 新しい金属酵素研究へのテラヘルツ電子スピン共鳴法の応用
2021 - 2024 Molecular basis for biosynthetic and degradation systems for tRNA sulfur-modified bases
2021 - 2024 Elucidation of the chemistry of gaseous molecule at iron binuclear center using nuclear resonance spectroscopies

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Papers (36)：

Masaki Horitani, Risa Yamada, Kanami Taroura, Akari Maeda, Toyoaki Anai, Satoshi Watanabe. Identification of Genes Responsible for the Synthesis of Glycitein Isoflavones in Soybean Seeds. Plants. 2024
Tatsuya Yamashita, Hiroki Matsuda, Kyohei Koizumi, Logu Thirumalaisamy, Myeongok Kim, Lumi Negishi, Hitoshi Kurumizaka, Yoriko Tominaga, Yoshihiro Takagi, Ken Takai, et al. Heme protein identified from scaly-foot gastropod can synthesize pyrite (FeS2) nanoparticles. Acta Biomaterialia. 2023. 162. 110-119
Hanae Takeda, Kanji Shimba, Masaki Horitani, Tetsunari Kimura, Takashi Nomura, Minoru Kubo, Yoshitsugu Shiro, Takehiko Tosha. Trapping of a Mononitrosyl Nonheme Intermediate of Nitric Oxide Reductase by Cryo-Photolysis of Caged Nitric Oxide. The journal of physical chemistry. B. 2023. 127. 4. 846-854
Masato Ishizaka, Minghao Chen, Shun Narai, Yoshikazu Tanaka, Toyoyuki Ose, Masaki Horitani, Min Yao. Quick and Spontaneous Transformation between [3Fe-4S] and [4Fe-4S] Iron-Sulfur Clusters in the tRNA-Thiolation Enzyme TtuA. International Journal of Molecular Sciences. 2023. 24. 1. 833-833
Kazuo Kobayashi, Yuko Tsutsui Ito, Yuri Kasu, Masaki Horitani, Takahiro Kozawa. Intramolecular electron transfer from biopterin to FeII-O2 complex in nitric oxide synthases occurs at very different rates between bacterial and mammalian enzymes: Direct observation of a catalytically active intermediate. Journal of inorganic biochemistry. 2023. 238. 112035-112035

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MISC (24)：

石坂優人, CHEN Minghao, 奈良井峻, 堀谷正樹, 田中良和, 田中良和, YAO Min, YAO Min. 鉄硫黄クラスターが関与するtRNAチオ化修飾酵素の詳細な反応機構解明を目指した研究. Photon Factory News. 2019. 37. 3
石坂優人, 陳明皓, 奈良井峻, 堀谷正樹, 田中良和, 田中良和, 姚閔. tRNA硫黄修飾酵素の活性と鉄硫黄クラスター構造の関連性. 日本細胞生物学会大会(Web). 2019. 71st. ROMBUNNO.1P-006 (WEB ONLY)
Masaki Horitani. Trapping of the Reaction Intermediates and Analysis of Enzymatic Catalytic reaction on Radical SAM Enzymes. Seibutsu Butsuri. 2017. 57. 4. 202-204
先端磁気共鳴法による金属タンパク質研究. 2017. 15
堀谷正樹. 先端磁気共鳴法による金属タンパク質研究. 電子スピンサイエンス学会年会講演要旨集. 2016. 55th. 66-67

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Lectures and oral presentations (26)：

好冷細菌由来無機ピロフォスファターゼの低温適応・熱安定性機構
(日本生体エネルギー研究会第45回討論会 2019)
好冷細菌由来無機ピロフォスファターゼの低温適応機構
(第58回NMR討論会・SEST2019連合大会 2019)
ラジカルSAM酵素におけるラジカル反応機構~自然界はどのようにしてラジカルを安全に利用しているか~
(2019年度第一回水和ナノ構造研究会 2019)
Cold Adaption Inorganic Pyrophosphatase from Shewanella sp. AS-11 was Investigated the Reaction Mechanism by X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy
(International Congress on Pure & Applied Chemistry (ICPAC) Yangon 2019 2019)
EPR Spectroscopy Reveals the Active Site Rearrangement of Di-Mn2+ Center by Substrate Binding in Inorganic Pyrophosphatase from Shewanella sp. AS-11
(Awaji Island Conference on Electron Spin Science & Technology 2019)

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Works (21)：

新規な酸素感受性tRNA硫黄化酵素の機能解析
2018 - 2018
Elucidation of the tRNAsulfurationmechanism of TtuA involved inFe-S cluster
Masato Ishizaka, Minghao Chen, Syun Narai, Masaki Horitani, Seiko Oka, YoshikzauTanaka, Min Yao 2018 - 2018
低温適応酵素グルコキナーゼの低温機構解明
2018 - 2018
低温適応酵素グルコキナーゼの低温機構解明に向けて
2017 - 2017
電子スピン共鳴法による低温適応無機ピロフォスファターゼ活性部位Mn2+イオンの電子状態解析
2017 - 2017

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Education (1)：

2004 - 2008 Osaka University Graduate School of Engineering Science Department of Mechanical Science and Bioengineering Division of Bioengineering

Professional career (1)：

博士(理学) (大阪大学)

Work history (5)：

2022/04 - 現在 Saga University Faculty of Agriculture Associate Professor
2016/12 - 2022/03 Saga University Faculty of Agriculture Assistant Professor
2016/12 - 2021/03 文部科学省卓越研究員
2010/09 - 2016/11 Northwestern University Department of Chemistry Postdoctral Fellow
2008/08 - 2010/08 理化学研究所播磨研究所特別研究員

Awards (1)：

2016/11 - The Society of Electron Spin Science and Technology SEST Young Investigator Award Advanced Magnetic Resonance Studies on Metalloproteins

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