Hirota Shun

ヒロタシュン | Hirota Shun

Affiliation and department：

Research field (2)： Bio-, chemical, and soft-matter physics , Structural biochemistry

Research keywords (1)： BIomolecular Chemistry, Bioinorganinc Chemistry, Metalloprotein, Protein Supramolecule, Reaction Mechanism

Research theme for competitive and other funds (27)：

2021 - 2025 Comprehensive understanding and application of protein 3D domain swapping
2021 - 2024 タンパク質の動きが連携して機能発現するバイオナノマシンの構築
2018 - 2022 In vivo formation and new design of protein oligomers based on 3D domain swapping
2018 - 2021 Elucidation of the amyloid formation mechanism of cytochrome c using the laser trapping method
2018 - 2021 Development of the rapid cell imaging system which can identify the chemical species without labeling by inverse Raman spectroscopy

2016 - 2018 Creation of Functional Protein Oligomers Utilizing the Structural Change of the Flexible Loop Region

2015 - 2017 光活性化を利用した[NiFe]ヒドロゲナーゼの触媒反応機構の分光学的研究

2015 - 2017 Development of new protein cages using domain swapping

2015 - 2017 Construction of metallo-catalysts with high reactivitites based on characters of selenoamino acid

2014 - 2017 Development of supramolecular construction techniques of proteins and their functionalization using domain swapping

2012 - 2015 Clarification of action mechanism of caspase-activating molecules and cancer cell-selective induction of apoptosis

2011 - 2013 揺らぎによるシトクロムc多量体形成と機能

2009 - 2012 Creation and Structure-Function Control of Photo-Reactive Proteins and Peptides

2008 - 2009 ヘモシアニンのアロステリック効果の分光学的研究

2007 - 2008 Research on Oxygen Binding Properties of Type 3 Copper Proteins

2006 - 2007 光解離性修飾基を利用したタンパク質のフォールディング反応の追跡

2005 - 2007 Preparation of Heme-Protein Based Birtatslysts with High Enzymatic Activity

2004 - 2006 Structual formation and structure-function relationship of protein studied with the use peptides

2004 - 2005 光解離性修飾基導入による蛋白質の構造と機能の研究

2000 - 2001 リシンペプチドとの相互作用による電子伝達タンパク質の金属活性部位の構造変化

2000 - 2001 Structural study of [NiFe]hydrogenase

1999 - 2001 Characterization of different enzymes catalyzing oxidative deamination of amines

2000 - 2000 弱い相互作用を利用した金属錯体の特性と組織化

1998 - 1999 アミン酸化酵素の脱アミン反応機構への分光学的アプローチ

1997 - 1999 Functions of Aromatic Rings in the Metal Coordination Sphere.

1997 - 1999 Studies on Bioactive Metal Complex in Dynamic Processes

1998 - 1998 金属錯体-芳香環系における弱い相互作用と自己組織化

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Papers (272)：

Takahiro Sakai, Tsuyoshi Mashima, Naoya Kobayashi, Hideaki Ogata, Lian Duan, Ryo Fujiki, Kowit Hengphasatporn, Taizo Uda, Yasuteru Shigeta, Emi Hifumi, et al. Structural and thermodynamic insights into antibody light chain tetramer formation through 3D domain swapping. Nature Communications. 2023. 14. 1. 7807-7807
Mohan Zhang, Hulin Tai, Sachiko Yanagisawa, Masaru Yamanaka, Takashi Ogura, Shun Hirota. Resonance Raman Studies on Heme Ligand Stretching Modes in Methionine80-Depleted Cytochrome c: Fe-His, Fe-O₂, and O-O Stretching Modes. The Journal of Physical Chemistry B. 2023. 127. 11. 2441-2449
Shun Hirota, Chun-Liang Chiu, Chieh-Ju Chang, Pei-Hua Lo, Tien Chen, Hongxu Yang, Masaru Yamanaka, Tsuyoshi Mashima, Cheng Xie, Hiroshi Masuhara, et al. Structural region essential for amyloid fibril formation in cytochrome c elucidated by optical trapping. Chemical Communications. 2022. 58. 92. 12839-12842
Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Léger, Silke Leimkühler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata, Markus W. Ribbe. Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase. Chemical Reviews. 2022. 122. 14. 11900-11973
Shigenori Nagatomo, Mitsuo Shoji, Takuto Terada, Kiyoharu Nakatani, Yasuteru Shigeta, Shun Hirota, Sachiko Yanagisawa, Minoru Kubo, Teizo Kitagawa, Masako Nagai, et al. Heme-bound tyrosine vibrations in hemoglobin M: Resonance Raman, crystallography, and DFT calculation. Biophysical Journal. 2022. 121. 14. 2767-2780

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MISC (30)：

Yuyama Ken-ichi, Ueda Mariko, Nagao Satoshi, Hirota Shun, Sugiyama Teruki, Masuhara Hiroshi. A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping. 2017
Hirota Shun, Yamashiro Nobuhiro, Wang Zhonghua, Nagao Satoshi. Effect of methionine80 heme coordination on domain swapping of cytochrome c. 2017
Kobayashi Hisashi, Nagao Satoshi, Hirota Shun. Characterization of the Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR. 2016
Lin Ying-Wu, Nagao Satoshi, Zhang Mohan, Shomura Yasuhito, Higuchi Yoshiki, Hirota Shun. Rational Design of Heterodimeric Protein using Domain Swapping for Myoglobin. 2014
Tai Hulin, Nishikawa Koji, Suzuki Masayuki, Higuchi Yoshiki, Hirota Shun. Control of the Transition between Ni-C and Ni-SIa States by the Redox State of the Proximal Fe-S Cluster in the Catalytic Cycle of [NiFe] Hydrogenase. 2014

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Books (13)：

CSJカレントレビュー36 生体分子反応を制御する : 化学的手法による機構と反応場の解明
化学同人 2020 ISBN:9784759813968
月刊化学 2月号 2020年の化学:最新のトピックス(ここまできた!!タンパク質設計-人工酵素、超分子創製からコンピュータデザインまで)
化学同人, 75(3), 70-71 2020
薬学教科書シリーズ3:物理化学
化学同人 2018
ブルーバックス元素118の新知識
講談社 2017
フロンティア生物無機化学
三共出版 2016

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Lectures and oral presentations (533)：

Constructing Heme Protein Supramolecules by 3D Domain Swapping
(2nd International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering 2019)
Domain Swapping of Azurin from Alcaligenes xylosoxidans and Characterization of Its Dimer
(第13回バイオ関連化学シンポジウム 2019)
フェリチンゲルの作製と金属吸着
(第13回バイオ関連化学シンポジウム 2019)
ドメインスワッピングを利用したミオグロビンへの金属結合部位の導入
(第13回バイオ関連化学シンポジウム 2019)
Microorganisms build the best fuel efficient hydrogen cells
(EurekAlert!( AAAS) 2019)

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Education (3)：

1992 - 1995 The Graduate University for Advanced Studies School of Mathematical and Physical Science Department of Functional Molecular Science
1990 - 1992 Kyoto University Graduate School of Engineering Department of Molecular Engineering
1986 - 1990 Kyoto University Faculty of Engineering Department of Hydrocarbon Chemistry

Professional career (1)：

博士(理学) (総合研究大学院大学)

Work history (6)：

2018/04 - Nara Institute of Science and Technology Division of Materials Science, Graduate School of Science and Technology Professor
2007/04 - 2018/03 Nara Institute of Science and Technology Graduate School of Materials Science Professor
2002/10 - 2007/03 Kyoto Pharmaceutical University Pharmaceutical Sciences Associate Professor
1996/04 - 2002/09 Nagoya University Graduate School of Science Assistant Professor
1995/10 - 1996/03 Postdoctoral Fellow Emory Univeristy Department of Chemistry

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Awards (6)：

2020/12 - 文部科学省ナノテクノロジープラットフォー令和2年度秀でた利用成果「優秀賞」光圧によるアミロイド線維の人工作製
2020/03 - Chemicaly Society of Japan The Chemical Society of Japan Award for Creative Work for 2019 Reaction Mechanism Elucidation and Supramolecular Creation of Metalloproteins
2015/09 - Chemical Society of Japan BCSJ Award Effect of a Procaspase-activating Compound on the Catalytic Activity of Mature Caspase-3
2006/02 - 日本薬学会薬学研究ビジョン部会賞タンパク質構造変化の新規測定法と制御法の開発
2000/03 - Chemicaly Society of Japan The Chemical Society of Japan Award For Young Chemists for 1999

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