Okada Satoshi

オカダサトシ | Okada Satoshi

Affiliation and department：
Job title：助教

Research field (4)： Molecular biology , Cell biology , Genomics , Systems genomics

Research keywords (6)： Molecular biology , Cell biology , Yeast genetics , Live cell imaging , Genome editing , CRISPR/Cas systems

Research theme for competitive and other funds (10)：

2024 - 2027 構造多型への構成的アプローチ:Cas9ニッケースとスプリントDNAによる誘導
2024 - 2027 独自に発見したCRISPR-Casエフェクターの機能構造解析と新たなゲノム編集技術の開発
2021 - 2024 Measurement of histone modification propagation velocity in a single living cell using CRISPR and BiFC
2022 - 2024 新規CRISPR/Casシステムを利用したゲノム編集ツール開発
2018 - 2021 Development of a live cell imaging method for visualization of epigenetic dynamics at a single locus

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Papers (17)：

Shunsuke Matsumoto, Suzuka Ono, Saori Shinoda, Chika Kakuta, Satoshi Okada, Takashi Ito, Tomoyuki Numata, Toshiya Endo. GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER. Journal of Cell Biology. 2022. 221. 6
Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito. Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae. G3 Genes|Genomes|Genetics. 2021. 11. 12
Goro Doi, Satoshi Okada, Takehiro Yasukawa, Yuki Sugiyama, Siqin Bala, Shintaro Miyazaki, Dongchon Kang, Takashi Ito. Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability. Nucleic Acids Research. 2021. 49. 2. 954-968
Younghoon Oh, Jennifer H. Schreiter, Hiroki Okada, Carsten Wloka, Satoshi Okada, Di Yan, Xudong Duan, Erfei Bi. Hof1 and Chs4 Interact via F-BAR Domain and Sel1-like Repeats to Control Extracellular Matrix Deposition during Cytokinesis. CURRENT BIOLOGY. 2017. 27. 18. 2878-+
Satoshi Okada, Mid Eum Lee, Erfei Bi, Hay-Oak Park. Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor. Methods in Enzymology. 2017. 589. 171-190

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MISC (3)：

Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito. Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae. bioRxiv. 2021
Younghoon Oh, Jennifer H. Schreiter, Hiroki Okada, Carsten Wloka, Satoshi Okada, Di Yan, Erfei Bi. Hof1 and Chs4 interact via F-BAR domain and Sel1-like repeats to control extracellular matrix deposition during cytokinesis in budding yeast. Cell Press Sneak Peak. 2018
Satoshi Okada, Erfei Bi. Daughter cell identity emerges from the interplay of Cdc42, septins, and exocytosis. 2013

Books (1)：

遺伝学事典
朝倉書店 2005 ISBN:4254171242

Lectures and oral presentations (49)：

Effective sgRNA search method for Cas9 nickase-based tandem gene array expansion
(2024)
Cas9ニッケースの複数遺伝子座への同時誘導による高度な遺伝子増幅系の構築
(日本農芸化学会2024年度大会 2024)
Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12 vector series with multiple selection markers enabling single-step multiplex genome editing in budding yeast
(The Allied Genetics Conference 2024 (TAGC24) 2024)
Strategic targeting of Cas9 nickase induces large segmental duplications
(The Allied Genetics Conference 2024 (TAGC24) 2024)
Cas9ニッケース誘導型縦列反復構造伸⻑法BiTRExの応用を考える
(第46回日本分子生物学会年会 2023)

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Works (4)：

第96回日本生化学会大会ウェブサイトおよびポスター電子顕微鏡画像提供・デザイン協力
岡田悟 2022 -
第15回日本エピジェネティクス研究会年会プログラム抄録集表紙デザイン
岡田悟 2022 -
第15回日本エピジェネティクス研究会年会ウェブサイトイラストデザイン・色彩設計
岡田悟 2022 -
Developmental Cell誌 (Volume 26 Number 2) 表紙デザイン
岡田悟 2013 -

Education (3)：

2004 - 2009 The University of Tokyo Graduate School of Frontier Sciences Department of Computational Biology
2002 - 2004 The University of Tokyo Graduate School of Frontier Sciences Department of Integrated Biosciences
1998 - 2002 The University of Tokyo

Professional career (1)：

Ph.D. (The University of Tokyo)

Work history (4)：

2014/03 - 現在 Kyushu University Graduate School of Medical Sciences Assistant Professor
2010/01 - 2014/03 University of Pennsylvania Perelman School of Medicine Postdoc
2009/04 - 2009/12 The University of Tokyo
2007/04 - 2009/03 The University of Tokyo

Committee career (3)：

2023/05 - 2023/11 第96回日本生化学会大会副幹事
2019/09 - 2021/12 酵母研究若手の会運営委員
2019 - 2019 酵母研究ルネッサンスオーガナイザー

Awards (4)：

2023/03 - 日本農芸化学会日本農芸化学会 2023年度大会トピックス賞 Cas9ニッケースの戦略的配置による汎用的な遺伝子増幅法の開発
2022/09 - 酵母遺伝学フォーラム学生発表賞優秀ポスター発表賞 Cas9変異体を用いて長大なゲノム領域の重複を誘導する
2017/12 - 国際学会派遣支援 Development of a BiFC system based on a bright and photo-stable fluorescent protein for detecting a limited number of protein-protein interactions
2013/12 - The Molecular Biology Society of Japan Travel Grants for Early Career Researchers in Overseas Establishment and maintenance of mother-daughter identities: interplay between Cdc42, septins, exocytosis in budding yeast

Association Membership(s) (7)：

酵母遺伝学フォーラム , 日本エピジェネティクス研究会 , The American Society for Cell Biology , THE MOLECULAR BIOLOGY SOCIETY OF JAPAN , Genetics Society of America , 日本生化学会 , 日本ゲノム編集学会

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